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Application of transgenic Lactobacillus with phytase gene as pig feed additive

A pig feed additive, Lactobacillus technology, applied in the application field of phytase gene-transferred Lactobacillus as a pig feed additive, can solve problems such as decline, intestinal microbial flora imbalance, animal disease production performance, etc., to reduce pollution and improve Effect of lower digestibility, phosphorus content

Inactive Publication Date: 2010-03-31
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to physiological factors and environmental stress, the intestinal microbial flora can be unbalanced, causing a large number of pathogenic microorganisms to multiply, which eventually leads to animal diseases and reduced production performance.

Method used

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  • Application of transgenic Lactobacillus with phytase gene as pig feed additive
  • Application of transgenic Lactobacillus with phytase gene as pig feed additive
  • Application of transgenic Lactobacillus with phytase gene as pig feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Application of embodiment 1 transgenic lactic acid bacteria in piglet feeding

[0016] One, the preparation method of phytase gene transgenic lactobacillus

[0017] 1 Extraction of prokaryotic expression vector plasmid pIAβ8

[0018] Pick a single colony containing the prokaryotic expression vector plasmid pIAβ8, inoculate it in LB liquid medium containing Amp (the volume of the medium should not exceed 20% of the capacity of the container), and cultivate overnight. Resin type for next day use TM Plasmid DNA extraction kit to extract plasmids. The materials and methods are described in previous chapters.

[0019] 2 Preparation of Lactobacillus Competent Cells

[0020] (1) Pick a single colony of Lactobacillus, and culture it statically for 12-16 hours in 3ml of MRS medium at 37°C.

[0021] (2) Dilute the overnight culture at a ratio of 1:50, and culture it statically at 37°C for 3.5-4.0h to make the culture OD 600 Reached around 0.5.

[0022] (3) Immediately take...

Embodiment 2

[0048] Application of Example 2 Transgenic Lactobacillus in Lean Meat Pigs

[0049] 1. The preparation method in Example 1 is used to prepare the phytase gene transgenic Lactobacillus additive.

[0050] 2. The application of phytase gene-transferred Lactobacillus as a pig feed additive is as follows:

[0051] Remove 22% of calcium hydrogen phosphate in the basic diet of pigs, and replace it with the same amount of stone powder and secondary powder, and the ratio of stone powder and secondary powder to replace 22% of calcium hydrogen phosphate is: 12% stone powder + 10% bran, basic The diet composition and nutrient levels are shown in Table 2. Add the phytase gene-embedded lactic acid bacteria additive of the present invention in the basal ration obtained above, and the addition amount is to add 600 grams of phytase gene-embedded lactic acid bacteria per ton of basal ration. The lean-meat pigs were fed with feed, and the feeding method of free access to food and free drinking...

Embodiment 3

[0052] Application of embodiment 3 transgenic lactic acid bacteria in fattening pigs

[0053] 1. The preparation method in Example 1 is used to prepare the phytase gene transgenic Lactobacillus additive.

[0054] 2. The application of phytase gene-transferred Lactobacillus as a pig feed additive is as follows:

[0055] Remove 25% of the calcium hydrogen phosphate in the basic diet of pigs and replace it with the same amount of stone powder and secondary powder. The ratio of stone powder and secondary powder to replace 25% of calcium hydrogen phosphate is: 15% stone powder + 10% bran, basic The diet composition and nutrient levels are shown in Table 2. Add the phytase gene-embedded lactic acid bacteria additive of the present invention in the basal ration obtained above, and the addition amount is to add 800 grams of phytase gene-embedded lactic acid bacteria per ton of basal ration. The fattening pigs were fed with feed, and the feeding method of free access to food and free...

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Abstract

The invention relates to an application of transgenic Lactobacillus with phytase gene as pig feed additive, the product can improve digestion and absorption of crude protein, crude fat, dry matter calcium and phosphor in pigling body, thereby improving digestion rate of nutrients, and imultaneously, reducing pH value of night soil, and improving emission amount of ammonia and nitrogen in night soil. Because content of phosphor in manure is reduced, pollution of environment is reduced. Transgenic Lactobacillus with phytase gene can substantially increase triglycerides content in blood, withoutsignificant influence for other serology indexes. Compared with contrast group, the content of phosphor in serum increases 10.5%; compared with contrast group, the content of Ig A in serum increases 120%, compared with antibiotic group, the content of Ig A in serum increases 18%, which demonstrates that malolactic bacteria has obvious function for improving animal immunity, and the efficacy is superior to antibiotic.

Description

technical field [0001] The invention relates to a pig feed additive, in particular to the application of a phytase gene-transformed lactobacillus as a pig feed additive. Background technique [0002] A large number of lactic acid bacteria exist in the intestinal tract of animals, and people recognized the importance of lactic acid bacteria to maintain the balance of the biota earlier. Therefore, lactic acid bacteria are currently the most widely used and most widely studied type of probiotics. For the regulation of intestinal microorganisms and In other words, probiotics are a new concept. In pig diets, probiotics are usually used to replace antibiotics in the diet. The effect of probiotics on the microorganisms in the digestive tract is just the opposite of that of antibiotics. Usually, antibiotics cure diseases by inhibiting all microorganisms. and disease prevention, while probiotics can cure and prevent diseases by regulating the microbial flora in the digestive tract of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A61K35/74A23K1/18A23K10/18A23K50/30
Inventor 尹清强左瑞雨路玲魏向阳郑秋红常娟陈丽颖任广志杨霞孙俊伟冯华
Owner HENAN AGRICULTURAL UNIVERSITY
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