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Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma

A cold plasma and generator technology, applied in the direction of hydrolase, can solve the problems of loss of activity and enzyme denaturation, and achieve the effect of improving the activity of lipase

Inactive Publication Date: 2011-05-11
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The usual severe physical treatment (such as ultraviolet light, temperature, pH adjustment, etc.) often denatures the enzyme and loses its activity

Method used

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  • Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma
  • Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma
  • Method for improving enzyme activity by utilizing atmospheric pressure discharge plasma

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Experimental program
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Embodiment Construction

[0013] Realize the steps of the inventive method as follows:

[0014] 1. Enzyme Solution Preparation

[0015] Dissolve the enzyme in the buffer (the type of buffer is selected according to the conditions required by the enzyme itself, and the crude enzyme solution needs to be dialyzed). The enzyme solution to be used needs to be stored at low temperature (-20°C ~ 4°C) for future use.

[0016] 2. Select the type of atmospheric pressure discharge cold plasma generator and set the working parameters

[0017] The atmospheric pressure cooling plasma generator used in the present invention is an atmospheric pressure glow discharge cooling plasma generator using two water-cooled bare metal electrodes. Atmospheric pressure cooling plasma generators can also use single or multiple water-cooled metal electrodes coated with insulating dielectric layers, atmospheric pressure dielectric barrier discharge cold plasma generators, and single or multiple resistors coated with high resistivit...

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PUM

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Abstract

The invention relates to a method for improving the enzyme activity by utilizing an atmospheric pressure discharge plasma, belonging to the field of cold plasma technology application. The method is characterized by comprising the following steps in sequence: preparing enzyme solution, selecting the type of an atmospheric pressure discharge plasma generator and setting working parameters, and treating the enzyme solution by utilizing the atmospheric pressure discharge plasma. The invention has the advantages of high efficiency and convenient change of the enzyme activity.

Description

technical field [0001] The invention relates to the application of cold plasma technology in the field of microorganisms, in particular to the application of cold plasma in controlling enzyme catalytic activity. Background technique [0002] Plasma is the fourth state of matter. It is a system composed of a large number of particles such as molecules, atoms, ions, and electrons. Since positive particles and negative particles have equal charges, they are macroscopically electrically neutral. Cold plasma is a kind of non-equilibrium plasma. The active particles have high energy and can maintain high enough chemical activity at low temperature (less than 50°C). Therefore, it has a very broad application prospect in the field of biochemical industry. [0003] Enzyme is a special protein with catalytic activity and high specificity. Environmental factors have a great influence on its stability and activity. High temperature, high pressure, strong acid, strong alkali or ultraviol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/20
Inventor 李和平王立言李果邢新会乐沛思包成玉冯权
Owner TSINGHUA UNIV
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