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Application of stabilizer trehalose in pig hepatitis E diagnostic kit

A diagnostic kit and trehalose technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low specificity and inability to specifically reflect the prevalence of pig hepatitis E, and achieve the effect of maintaining stability

Inactive Publication Date: 2010-05-19
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, there is no epitope research report of porcine hepatitis E virus at present. The kits used for the detection of porcine hepatitis E virus on the market are the kits for the detection of human hepatitis E virus. Although the sequence homology of porcine hepatitis E virus and human hepatitis E virus Very high, but there are some differences between them after all. Using human hepatitis E virus epitopes to detect porcine hepatitis E virus is not very specific, and cannot specifically reflect the prevalence of porcine hepatitis E virus

Method used

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  • Application of stabilizer trehalose in pig hepatitis E diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Determination of Stability of ELISA Plates Adding and Not Adding Stabilizer Trehalose in Enzyme Conjugate Diluent

[0029] (1) Antigen dilution

[0030] Take 10 μl of the polypeptide with a concentration of 10 mg / ml (dissolved 5 mg of the synthesized polypeptide in 0.5 ml of DMSO DMSO) and mix it with 90 μl of PBS solution to obtain an antigen dilution with a solubility of 1 ug / μl. Then take 15 μl of the diluted antigen solution and mix with 5 ml of the coating solution to obtain a diluted antigen solubility of 3 μg / ml. Add antigen dilution to 100 μl / well on the microtiter plate, and coat at 4°C for 12 hours.

[0031] (2) closed

[0032] The coating solution was sucked off, and 200 μl of blocking solution was added to each well to block at 37°C for 2 hours.

[0033] (3) Add primary antibody

[0034]Wash the plate 3 times with TBST solution, add 100 μl of sample diluent and 10 μl of serum to each well, shake and mix well, and incubate at 37°C for 1 hour.

[0035] (4...

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Abstract

The invention discloses an application of stabilizer trehalose in a pig hepatitis E diagnostic kit; 8-12wt percent of trehalose is added in enzyme combination diluent of the pig hepatitis E diagnostic kit, the function of the enzyme combination diluent is kept to be stable by utilizing the stabilizer trehalose, so as to keep the stabilization of the pig hepatitis E diagnostic kit.

Description

technical field [0001] The invention relates to the application of a stabilizer trehalose in a porcine hepatitis E diagnostic kit. Background technique [0002] Hepatitis E virus (HEV) is a non-A-non-B hepatitis virus transmitted through the fecal-oral route. It can cause outbreaks in the crowd, mainly affecting young adults, and can cause 20% fatality rate in pregnant women. From 1986 to 1988, the hepatitis E epidemic was caused by drinking water pollution in southern Xinjiang, my country, with a total of 119,280 cases and 707 deaths. It is the largest hepatitis E epidemic in the world so far. In recent years, the incidence of hepatitis E has been increasing year by year. According to the statistics of the Ministry of Health of my country, the incidence of hepatitis E in 2003 increased by 40.4% compared with that in 2002. Therefore, the prevention and control of hepatitis E has been included in the work schedule of public health institutions at all levels. [0003] In rece...

Claims

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Application Information

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IPC IPC(8): G01N33/576
Inventor 赵凯刘启文李震于瑞嵩唐雪明朱宏谭芙蓉吴潇王金斌陶世如蒋玲曦
Owner SHANGHAI ACAD OF AGRI SCI
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