Ultralow-temperature preservation method of rachycentron canadum spermia

A technology for ultra-low temperature preservation and cobia, which is applied in the preservation of human or animal body, fish farming, climate change adaptation, etc., to achieve the effects of improving utilization, improving economic benefits, and increasing the number of seedlings.

Inactive Publication Date: 2010-05-26
GUANGDONG OCEAN UNIVERSITY
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  • Ultralow-temperature preservation method of rachycentron canadum spermia
  • Ultralow-temperature preservation method of rachycentron canadum spermia

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[0013] Accurately prepare the sperm preservation base solution and sperm activation solution, the formula and preparation method of the sperm preservation solution: first prepare the sperm preservation base solution, the formula of the sperm preservation base solution: sodium chloride (NaCl) 65 ~ 75mmol / l, potassium chloride ( KCl) 1.45~1.5mmol / l, calcium chloride (CaCl 2 )2.5~2.7mmol / l, sodium carbonate (NaHCO 3 )20~25mmol / l, magnesium chloride (MgCl 2 ) 5.7~6.1mmol / l, glucose 150~200mmol / l, calf serum 9.4~10mg / ml, prepare the sperm preservation base solution according to the formula, and adjust the pH value of the sperm preservation base solution with 1mol / l sodium bicarbonate to 7.8, and then add 9-10% of the volume of Dimethyridine as an antifreezing agent to obtain a sperm preservation solution.

[0014] The formula of sperm activation liquid: described activation liquid formula is: sodium chloride (NaCl) 8g / l, potassium chloride (KCl) 0.4g / l, calcium chloride (CaC 12 ...

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Abstract

The invention belongs to the technical field of aquaculture, in particular to an ultralow-temperature preservation method of rachycentron canadum spermia, comprising the following steps of: diluting and uniformly mixing the collected fresh seminal fluid and spemium preserving fluid step by step according to the volume ratio of 1:3 within 20 seconds, placing the mixture on the liquid level of liquid nitrogen for precooling for 5 minutes at the precooling temperature of 70 DEG C below zero to80 DEG C below zero, and then placing the mixture in the liquid nitrogen with 196 DEG C below zero for long-time preservation. When in use, the preserved spermia are unfrozen at the temperature of 37 to 40 DEG C for 3 to 4 seconds, are immigrated into a spermium activating fluid for activation and then are inseminated. The ultralow-temperature preservation method of the rachycentron canadum spermia in the invention contributes to enhancing the utilization rate of the rachycentron canadum spermia so as to increase the fingerling breeding quantity and enhance the economic benefits. The technology can be used for the artificial breeding of fish, and also has the important significance on developing the recovery and reestablishment of fish resources.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, in particular to a method for cryopreservation of cobia sperm. Background technique [0002] At present, all the seedlings needed for cobia breeding and production come from artificial breeding, and artificial breeding often has the problem that the maturity of male and female fish is inconsistent or the sex ratio is improper, which leads to the problem that the sperm cannot be fully utilized. Moreover, there are seasonal changes in the quality and quantity of fish sperm, resulting in insufficient fertilization rates in stages. Therefore, the development and application of sperm preservation technology will help to improve the utilization rate of cobia sperm, increase the breeding capacity of seedlings, and improve economic benefits. [0003] Regarding the preservation of fish sperm, it is generally divided into three methods: normal temperature, low temperature and ultra-low temperature. N...

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Application Information

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IPC IPC(8): A01N1/02A01K61/00C12N5/076
CPCY02A40/81
Inventor 汤保贵陈刚张建东周晖施钢潘传豪黄建盛
Owner GUANGDONG OCEAN UNIVERSITY
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