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Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen

A liquid culture medium, in vitro germination technology, applied in measurement devices, plant cells, instruments, etc., to achieve the effect of easy observation and measurement, and stable and reliable measurement results.

Inactive Publication Date: 2010-06-02
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, there are no reports on the in vitro germination of Scutellaria baicalensis pollen and the growth of pollen tubes at home and abroad.

Method used

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  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen
  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen
  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen

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Embodiment Construction

[0026] According to the technical scheme of the present invention, firstly, prepare a liquid culture medium for in vitro germination of Scutellaria baicalensis pollen, which uses distilled water as a solvent and contains 100 g / L of sucrose, 150 g / L of PEG, and BK, The pH value is 5.8;

[0027] The BK consists of the following raw materials: H 3 BO 3 : 100mg / L, Ca(NO 3 ) 2 4H 2 O: 300mg / L, MgSO 4 ·7H 2 O: 200mg / L, KNO 3 : 100mg / L.

[0028] On the basis of determining the liquid medium for in vitro germination of Scutellaria baicalensis pollen, use the liquid medium for in vitro germination of Scutellaria baicalensis pollen to culture Scutellaria baicalensis pollen in vitro, measure the germination rate of pollen and the growth of pollen tubes, and can reliably and effectively determine the pollen vitality.

[0029] The following is an embodiment given by the inventor, which is a preferred example of the present invention and is mainly used to further explain and unders...

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Abstract

The invention discloses a liquid medium for vitro scutellaria root pollen germination and a method for testing the activity of scutellaria root pollen. For the liquid medium for vitro scutellaria root germination, distilled water is used as a solvent, and the liquid medium comprises the components of BK, 100g / L cane sugar and 150g / L PEG4,000, the pH value of the liquid medium is 5.8. The tested results of pollen activity is stable and reliable by utilizing the liquid medium for vitro scutellaria root pollen germination and a pollen tube growth testing technology, the ratio of the pollen germination reflects the proportion of pollens with vitality, and the observation and the measurement of the pollen tube reflect the advantages and disadvantages of physiologic conditions of pollen grains, thus the technology for testing vitro scutellaria root pollen germination and pollen tube growth provides a reliable and effective method for the measurement of the activity of the scutellaria root pollen germination.

Description

technical field [0001] The invention belongs to the technical field of measuring plant pollen vigor, and relates to a culture medium, in particular to a liquid medium for in vitro germination of scutellaria baicalensis pollen, and a method for measuring the vigor of baicalin baicalensis pollen by using the liquid medium for in vitro germination of baicalensis pollen. Background technique [0002] Scutellaria Baicalensis is a plant of Labiatae. There are more than 300 species of plants in the same genus, which are widely distributed all over the world. There are 101 species and 29 varieties in my country. Scutellaria baicalensis is bitter in taste and cold in nature. It has the effects of clearing heat, drying dampness, purging fire and detoxification, cooling blood to stop bleeding, removing heat and preventing miscarriage. It can also be used to make tea, which has the effects of cooling fire, reducing inflammation and heat. [0003] In recent years, as the pharmacological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04G01B11/02
Inventor 呼天明杨培志刘志刚
Owner NORTHWEST A & F UNIV
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