Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen

A liquid culture medium, in vitro germination technology, applied in measurement devices, plant cells, instruments, etc., to achieve the effect of easy observation and measurement, and stable and reliable measurement results.

Inactive Publication Date: 2010-06-02
NORTHWEST A & F UNIV
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the in vitro germination of Scutellaria baicalensis pollen and the growth of pollen tubes at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen
  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen
  • Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] According to the technical scheme of the present invention, firstly, prepare a liquid culture medium for in vitro germination of Scutellaria baicalensis pollen, which uses distilled water as a solvent and contains 100 g / L of sucrose, 150 g / L of PEG, and BK, The pH value is 5.8;

[0027] The BK consists of the following raw materials: H 3 BO 3 : 100mg / L, Ca(NO 3 ) 2 4H 2 O: 300mg / L, MgSO 4 ·7H 2 O: 200mg / L, KNO 3 : 100mg / L.

[0028] On the basis of determining the liquid medium for in vitro germination of Scutellaria baicalensis pollen, use the liquid medium for in vitro germination of Scutellaria baicalensis pollen to culture Scutellaria baicalensis pollen in vitro, measure the germination rate of pollen and the growth of pollen tubes, and can reliably and effectively determine the pollen vitality.

[0029] The following is an embodiment given by the inventor, which is a preferred example of the present invention and is mainly used to further explain and unders...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a liquid medium for vitro scutellaria root pollen germination and a method for testing the activity of scutellaria root pollen. For the liquid medium for vitro scutellaria root germination, distilled water is used as a solvent, and the liquid medium comprises the components of BK, 100g/L cane sugar and 150g/L PEG4,000, the pH value of the liquid medium is 5.8. The tested results of pollen activity is stable and reliable by utilizing the liquid medium for vitro scutellaria root pollen germination and a pollen tube growth testing technology, the ratio of the pollen germination reflects the proportion of pollens with vitality, and the observation and the measurement of the pollen tube reflect the advantages and disadvantages of physiologic conditions of pollen grains, thus the technology for testing vitro scutellaria root pollen germination and pollen tube growth provides a reliable and effective method for the measurement of the activity of the scutellaria root pollen germination.

Description

technical field [0001] The invention belongs to the technical field of measuring plant pollen vigor, and relates to a culture medium, in particular to a liquid medium for in vitro germination of scutellaria baicalensis pollen, and a method for measuring the vigor of baicalin baicalensis pollen by using the liquid medium for in vitro germination of baicalensis pollen. Background technique [0002] Scutellaria Baicalensis is a plant of Labiatae. There are more than 300 species of plants in the same genus, which are widely distributed all over the world. There are 101 species and 29 varieties in my country. Scutellaria baicalensis is bitter in taste and cold in nature. It has the effects of clearing heat, drying dampness, purging fire and detoxification, cooling blood to stop bleeding, removing heat and preventing miscarriage. It can also be used to make tea, which has the effects of cooling fire, reducing inflammation and heat. [0003] In recent years, as the pharmacological ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04G01B11/02
Inventor 呼天明杨培志刘志刚
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap