Method for extracting alga-inhibiting active ingredients from Artemisia annua L. and alga-inhibiting method
An algae inhibitory activity, Artemisia annua technology, applied in the directions of botanical equipment and methods, herbicides and algaecides, chemicals for biological control, etc. Low cost and the effect of expanding the scope of use
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Embodiment 1
[0017] The aboveground parts of Artemisia annua in the flowering period are collected, dried at 80°C for 48 hours, and crushed. Weigh 300g of the crushed Artemisia annua, and extract twice with 1.5L of petroleum ether under the assistance of 300W, 40KHz ultrasonic waves, each for 3 hours. Filter, combine the petroleum ether extracts, and then wash once with 1L 0.5-5%wt hydrochloric acid solution. The acid-washed petroleum ether extract is extracted twice with 2L 1-5% sodium hydroxide solution, the aqueous phase is combined and adjusted to pH=2~3 with concentrated hydrochloric acid or sulfuric acid, and extracted twice with 0.5L ethyl acetate , The ethyl acetate layers are combined and concentrated to obtain 6.5 g of the algae-inhibiting active ingredient.
[0018] The residue of Artemisia annua leached with petroleum ether was extracted twice with 1.5L of ethyl acetate under the assisting action of ultrasound, each for 3 hours. After filtering, the ethyl acetate layers are comb...
Embodiment 2
[0021] The aboveground parts of Artemisia annua in the flowering period are collected, dried at 80°C for 48 hours, and crushed. Weigh 60g of the crushed Artemisia annua L., use 350ml of n-hexane to extract with a Soxhlet extractor for 48 hours, and wash the n-hexane extract once with 200ml of 0.5-5% dilute acid. The acid-washed n-hexane extract was extracted with 300ml of 1-5% dilute alkali for 2 to 3 times. After the aqueous phase was combined, adjusted to pH = 2 to 3, and extracted twice with 200ml of ether. The ether layer was combined and concentrated to obtain a pH The active ingredient of algae is 1.12g.
[0022] The above-mentioned Artemisia annua residues extracted with n-hexane Soxhlet were extracted with 350 ml of ether Soxhlet for 48 hours. After extraction, the ether layer is washed once with 200ml of 0.5-5% dilute acid and 300ml of 1-5% dilute alkali for 2 to 3 times, and then the ether layer is concentrated to obtain 2.33g of the algae-inhibiting active ingredient....
Embodiment 3
[0025] Using the algae inhibitor obtained by the above method to conduct acute toxicity test on aseptically cultured Microcystis aeruginosa, set a certain concentration gradient for each drug, set a blank and two groups in parallel, observe once every 24 hours, and observe continuously for 5 days. The observation result is expressed as the inhibition rate of 96 hours. The results are as follows:
[0026] The algae-inhibiting active ingredient 1 obtained from Experimental Example 1, when the concentration reaches 100 mg / L, its inhibition rate on algae reaches 97%.
[0027] The second active ingredient of anti-algae obtained from Experimental Example 1, when the concentration reaches 150 mg / L, its inhibition rate on algae reaches 98%.
[0028] The mixed algae inhibitor obtained from Experimental Example 1: When the concentration reaches 60 mg / L, its inhibition rate on algae reaches 97%.
[0029] The mixed algae inhibitor obtained from Experimental Example 2: When the concentration reac...
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