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Method for carrying out streaming counting sort by utilizing micro light pattern

A cell and pattern technology, applied in the field of microfluidics and microfluidics biomedical analysis chips, can solve the problems of complex microelectrode structure, complex chip structure, high production cost, etc., to achieve flexible and efficient separation, improve efficiency, and reduce costs Effect

Inactive Publication Date: 2010-06-23
SOUTHEAST UNIV
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Problems solved by technology

[0005] Technical problem: The purpose of the present invention is to provide a method for flow counting and sorting cells using microscopic light patterns, so as to solve the problem of high cost of conventional flow analyzers and the conventional biological cell flow continuous micro-analysis chip microelectrodes. Defects with complex structure or chip structure and high production cost

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  • Method for carrying out streaming counting sort by utilizing micro light pattern
  • Method for carrying out streaming counting sort by utilizing micro light pattern
  • Method for carrying out streaming counting sort by utilizing micro light pattern

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Embodiment Construction

[0021] For an embodiment of the method for flow counting and sorting cells using microscopic light patterns provided by the present invention, see figure 1 with figure 2 . The chip structure, material and pattern of the light patterns that can be used in this method are not limited to this embodiment. The cell counting and sorting chip used in this embodiment comprises a sample inlet 11, a transparent insulating cover 12, an upper transparent indium tin oxide film 13, an intermediate spacer 2, a microfluidic chamber 21, a transparent insulating substrate 34, A photopattern dummy electrode formation layer composed of a silicon nitride layer 31 , a photoconductive layer 32 and a transparent conductive layer 33 . The silicon nitride layer 31 can prevent hydrolysis, and the photoconductive layer 32 has photoconductive properties, that is, the number of internal carriers increases sharply when illuminated by light, and the number of internal carriers is very small when it is not...

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Abstract

The invention relates to a method for carrying out streaming counting sort by utilizing a micro light pattern; a cell convergence channel, a single cell sampling recognition channel and a cell separation channel are formed by sequentially constructing the light pattern, so as to realize continuous convergence, recognition and separation of cells; in the cell recognition process, sorting counting of various cells is carried out, and the three channels form a sorting microsystem sequentially to realize the counting sort of various cells in an organism sample finally. The organism cell counting and separating method provided by the invention fully utilizes the flexibility of the micro light pattern, and a complex physical entity electrode array is avoided from being manufactured on the chip, and the method is superior to the existing counting sorting method of the organism cells on the cost, function and performance.

Description

technical field [0001] The invention relates to a method for flow counting and sorting biological cells by using a photoelectric microfluidic device, and relates to the field of microfluidics, in particular to the field of microfluidic biomedical analysis chips. Background technique [0002] Traditional flow cytometers have been widely used in the medical industry and biomedical research institutions. It mainly gathers cells into a single-cell queue and makes the cells pass through a flow-through detector one by one in order. At this time, the cells are in a narrow bundle. In the focusing light path of the cell, each cell will block the light beam once when it passes through, and a certain number of cells will interrupt the light beam continuously, so that the detector can detect the number of light blockages per unit time, so as to calculate the number of cells. The light scattering method commonly used in detection can also judge the volume and shape of each cell according...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N1/14
Inventor 常严朱晓璐易红倪中华
Owner SOUTHEAST UNIV
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