Cellulose decomposition compound bacteria system and method for using product of cellulose decomposition compound bacteria system to cultivate sulfate reducing bacteria
A cellulose decomposition and compound bacteria technology, applied in the field of microorganisms, can solve the problems of difficult decomposition, underutilized resources, and complex structure.
Inactive Publication Date: 2010-06-23
有研资源环境技术研究院(北京)有限公司
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Problems solved by technology
Due to its complex structure and difficult to decompose, most of these resources have not been fully utilized at present
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[0031] Firstly, a medium for enriching and cultivating cellulolytic bacteria is prepared. The medium was dispensed into anaerobic screw cap test tubes fitted with filter paper strips (12×120 mm). After vacuum pumping and oxygen removal, high pressure steam sterilization (121°C). Put 0.5g of anaerobic activated sludge into the sterilized culture medium, and put it into a 45°C incubator for static cultivation. After the filter paper was decomposed, the cells were successively transferred and cultured under the same culture conditions. During the transfer process, the ability of the bacteria to decompose the filter paper was continuously strengthened, and finally the filter paper strips could be completely muddied within 48 hours. Preserve the enriched and isolated cellulolytic strains (the name of the strain is Clostridium sp.Lwy-1, preservation unit: China National Center for Typical Culture Collection, address in Wuhan University, preservation date: October 17, 2008, Deposi...
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The invention provides a cellulose decomposition compound bacteria system and a method for using products of the cellulose decomposition compound bacteria system to cultivate sulfate reducing bacteria. The name of the cellulose decomposition compound bacteria system is Clostridium sp.Lwy-1, the preservation register number of the cellulose decomposition compound bacteria system is CCTCC NO:M208160, the preservation date of the cellulose decomposition compound bacteria system is on October 17th in 2008, the preservation unit of the cellulose decomposition compound bacteria system is China Center for Type Culture Collection, and the address of China Center for Type Culture Collection is in the Wuhan University. The bacteria have strong decomposition ability for the filter paper and also show stronger decomposition ability for the straw. The micromolecule products generated by the bacteria in the process of decomposing the straw can be used as a carbon source to cultivate the sulfate reducing bacteria (SRB). The invention has the advantage that the richest biological matter resource of China can be sufficiently used to make a method for using the sulfate reducing bacteria (SRB) to process the acid mine wastewater obtain greater development.
Description
technical field [0001] The invention relates to the technical field of microorganisms. In particular, it relates to a complex strain of anaerobic bacteria capable of decomposing cellulose, a cultivation method thereof, and a method for cultivating SRB (Sulfate-reducing Bacteria) by the strain decomposing rice straw as a carbon source. Background technique [0002] The method of using sulfate-reducing bacteria (SRB) to treat acid mine wastewater has the characteristics of low cost, strong applicability, no secondary pollution, and the ability to recover heavy metals in acid mine wastewater. The growth of SRB needs to be completed with an external carbon source. At present, some researches have been carried out at home and abroad to select cheap organic matter, even as waste, such as domestic wastewater and corncobs, as carbon sources to support the growth of SRB. [0003] Lignocellulose is the most abundant biomass resource in my country, including a large amount of crop str...
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Login to View More IPC IPC(8): C12N1/20C12R1/145C12R1/01
Inventor 刘文彦陈勃伟刘兴宇杨丽梅
Owner 有研资源环境技术研究院(北京)有限公司