Fluquinconazole-based synergistic pesticide composition
A pesticide composition, the technology of fluquinazole, applied in the field of pesticides, can solve the problems of easy resistance of bacteria and high cost of use, and achieve the effects of improving the control effect, reducing the amount of medication, and reducing costs
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example 1
[0028] Biological Assay Example 1: Indoor Toxicity Determination of Fluquinazole and Kresoxim-methyl Compound of Pear Scab
[0029] Test object: pear scab (collected from the field and separated and purified)
[0030] Test method: spore germination method. The pear scab was isolated from lesions collected from fresh fruits in the field. The fungus was continuously cultured on PDA medium in an incubator at 25°C for three times for purification, and then inoculated into PDB liquid medium to cultivate spores. The cultured pear scab spores were filtered with gauze to remove the hyphae, and then prepared into 4 × 10 with sterile water 5 spores / ml suspension. Dilute the test agent to the corresponding concentration, and mix it with the spore suspension of pear scab 2 mL plus 2 mL, and use 2 mL of sterile water plus 2 mL of the spore suspension as the blank control. Place them in a 24°C incubator for culture, check the spore germination rate after 15 hours, and calculate the relat...
example 2
[0041] Bioassay Example 2: Indoor Toxicity Determination of Fluquinazole and Enoxostrobin Compound on Apple Spotted Deciduous
[0042] Test object: Apple spotted leaf deciduous bacteria (collected from the field, and separated and purified)
[0043] Test method: mycelial growth rate method. Cultivate the bacterium Leptosphaeria spp. with PDA medium, and when the colonies just cover the petri dish, punch holes from the edge with a puncher with an inner diameter of 7 mm, and the resulting mycelium block is used as an inoculum. Take 5 mL of the prepared drug solution and mix them with quantitative 75 mL of sterilized medium to make 4 drug-containing plates, and use the same amount of sterile water mixed with the medium as a control. The mycelium block was placed upside down and placed in the center of the plate, and cultured in a 28°C incubator. After 7 days, the diameter of the colony was measured by the cross method, and the net growth of the colony and the inhibition rate of...
example 1
[0046] The mycelial growth inhibition rate was converted into a probability value (y), the concentration of the drug solution (μg / ml) was converted into a logarithmic value (x), and the toxicity equation and the inhibition concentration EC were calculated by the least square method 50 According to Sun Yunpei's method, the toxicity index and co-toxicity coefficient (CTC) of the drug were calculated. The calculation method is the same as the biological assay example 1. The results of the toxicity test are shown in Table 2.
[0047] The measurement results showed that the ratio of fluquinazole and erythoxystrobin between 40:1~1:50 had a synergistic effect on the apple spotted deciduous fungus, especially between 10:1~1:15, the synergistic effect Obviously, the co-toxicity coefficient is above 170.
[0048]Table 2 Indoor toxicity test results of fluquinazole and erythoxystrobin compound against apple spotted leaf deciduous
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