Aschersonia aleyrodis yellow non-woven cloth antibacterial and preparation method thereof

A technology of conchosporium whitefly and cloth inoculum, which is applied in the field of conchosporium whitefly yellow non-woven inoculum and its preparation, can solve the problems of declining market share, killing and injuring natural enemies, slow effect, etc. Long-lasting control effect and high success rate

Inactive Publication Date: 2010-07-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Netherlands and the former Czechoslovakia used blastospores to carry out small-scale experimental production through liquid fermentation culture. The main component of the product is blastospores, which are used for the control of whitefly and scale insects in greenhouses and other places. Due to the slow effect of this fungal preparation product, There are certain requirements for the use environment (such as high humidity requirements); during the development process, it was affected by the competitive pressure of chemical pesticides, resulting in a decline in market share
At present, there are reports on the mixture of this bacterium and chemical pesticides in my country (patent 200810029251.7 "Compound insecticide of Ascospora loboides and beta-cypermethrin", patent 200810029250.2 "Compound insecticide of Ascospora whitefly and abamectin Insecticide", patent 200810027583.1 "Compound insecticide of A. whitefly and acetamiprid", patent 200710031518.1 "Compound insecticide of A. whitefly and imidacloprid"), but these chemical pesticides are widely used in organic tea gardens and In the orchard, it is still forbidden, and it will also kill natural enemies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The production process method of the non-woven bar of the whitefly Ascospora whitefly includes five production processes:

[0039] 1. Strains:

[0040] The AsG0910 strain, whose preservation number is CGMCC No.3523, is isolated from Aschersonia aleyrodis of the citrus whitefly

[0041] 2. Culture of first-level slant bacteria:

[0042] Inoculate the isolated and purified bacteria on the slant of PDA medium, put it in a 25°C incubator, and cultivate it for 20-22 days. When the mycelia grows all over the slant and produces spores, transfer it to a shaker flask for culture;

[0043] 3. Liquid seed culture:

[0044] Put 200ml of liquid seed culture medium into a 500ml triangular flask, autoclave at 1.5pa / kg for 30min, cool to about 40°C, scrape two cultured ones from the slant fungus seeds in the ultra-clean workbench Put the spore powder of grade slant bacteria (test tube specification 180mm×15mm) into sterile Tween80 (volume concentration 0.01%) water, use a high-speed...

Embodiment 2

[0056] A. The acquisition of strains:

[0057] The AsG0910 strain, whose preservation number is CGMCC No.3523, is isolated from Aschersonia aleyrodis of the citrus whitefly

[0058] B. Culture of first-level slant strains:

[0059] Inoculate the isolated and purified strains on the slant of PDA medium, put them in an incubator at 25°C, and cultivate them for 20 days. When the mycelia grow all over the slant and produce spores, transfer them to shake flasks for liquid seed culture;

[0060] C. Liquid seed culture:

[0061] Put 200ml of liquid seed medium into a 500ml Erlenmeyer flask, autoclave at 1.5pa / kg for 30min, cool down to 40±1°C, and scrape 2 cultured cultured strains from the slant fungus seeds in the ultra-clean workbench Put the spore powder of the first-grade slant bacteria into the sterile Tween80 aqueous solution with a volume concentration of 0.01%, scrape the test tube with a size of 180mm×15mm, use a high-speed disperser to disperse the spores evenly, and the...

Embodiment 3

[0077] A. The acquisition of strains:

[0078] The AsG0910 strain, whose preservation number is CGMCC No.3523, is isolated from Aschersonia aleyrodis of the citrus whitefly

[0079] B. Culture of first-level slant strains:

[0080] Inoculate the isolated and purified strains on the slant of PDA medium, put them in an incubator at 25°C, and cultivate them for 22 days. When the mycelium grows all over the slant and produces spores, transfer it to a shaker flask for liquid seed culture;

[0081] C. Liquid seed culture:

[0082] Put 200ml of liquid seed medium into a 500ml Erlenmeyer flask, autoclave at 1.5pa / kg for 30min, cool down to 40±1°C, and scrape 2 cultured cultured strains from the slant fungus seeds in the ultra-clean workbench Put the spore powder of the first-grade slant bacteria into the sterile Tween80 aqueous solution with a volume concentration of 0.01%, scrape the test tube with a size of 180mm×15mm, use a high-speed disperser to disperse the spores evenly, and ...

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PUM

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Abstract

The invention relates to aschersonia aleyrodis yellow non-woven cloth antibacterial and a preparation method thereof, i.e. a production process method for producing an aschersonia aleyrodis yellow non-woven cloth antibacterial of a fungal pesticide for effectively preventing sucking pests of orange gardens and tea gardens. The method comprises five production procedures including stain obtainment, one-stage bevel strain culturing, liquid seed culturing, liquid fermentation tank production and inoculation culturing of a yellow non-woven cloth bacteria strip. The pesticide has lasting control effect on the pests, obvious and stable effect on killing aschersonia pests with tendency to yellow habit in the orange gardens and tea gardens, little influence by weather, stable process, little investment, small land occupation space and no three wastes in production.

Description

technical field [0001] The present invention relates to a new dosage form production method of insecticidal fungus, specifically a kind of Aschersonia aleyrodis AsG0910 strain isolated from citrus whitefly, which can effectively prevent and control the piercing-sucking mouthparts of orange orchards and tea gardens A production method of a yellow non-woven fabric bacterial agent for pests. Background technique [0002] The fast-acting ways in which chemical pesticides protect crops from damage also leave behind mounting consequences, raising a host of environmental and social concerns. The rapid increase of insecticide resistance, the continuous increase of control costs, the deterioration of the agricultural ecological environment, and the increasingly serious environmental pollution have formed a vicious circle in which the more pesticides are used, the more they are used, and the more used they are used. Therefore, the development of biopesticides and the application of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/00A01P7/04C12N1/14C12R1/645
Inventor 王联德尤民生黄建姜荣良林成辉
Owner FUJIAN AGRI & FORESTRY UNIV
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