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Immune liposome based on Isthmin gene as well as preparation method and application thereof

An immunoliposome and liposome technology, applied in the preparation of the immunoliposome, in the field of immunoliposomes based on the Isthmin gene, can solve the problem of increasing the intake, prolonging the action time of the encapsulation, and lacking active targeting and other problems, to achieve a simple preparation method, good application prospects, and the effect of inhibiting tumor angiogenesis

Inactive Publication Date: 2010-08-11
ARMY MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0005] Liposome is a spherical body encapsulated by one or more layers of concentric lipid bimolecular membranes, which can wrap various substances (such as drugs, nucleic acids, etc.) and transfer them into various types of cells. It has the characteristics of prolonging the action time of the wrap, increasing the uptake of the wrap by cells, improving the curative effect, and reducing toxic and side effects, but it lacks active targeting

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  • Immune liposome based on Isthmin gene as well as preparation method and application thereof
  • Immune liposome based on Isthmin gene as well as preparation method and application thereof
  • Immune liposome based on Isthmin gene as well as preparation method and application thereof

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Embodiment Construction

[0027] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0028] 1. Preparation of immunoliposome (Anti-vWF-Lip-Isthmin) based on Isthmin gene

[0029] 1. Preparation and detection of Isthmin recombinant eukaryotic expression vector

[0030] (1) Preparation of Isthmin recombinant eukaryotic expression vector

[0031] Take mouse spleen tissue, add liquid nitrogen to fully grind, weigh 100 mg and put it in an RNase-free centrifuge tube, use an RNA extraction kit (TaKaRa company) to extract total RNA from vascular endothelial cells, and operate according ...

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Abstract

The invention discloses an immune liposome based on Isthmin gene as well as a preparation method and application thereof. The immune liposome is formed by covalently combining a liposome encapsulated with an Isthmin recombinant eukaryotic expression vector and an antigen monoclonal antibody related to an anti-VIIIth factor. The preparation method comprises three steps of the preparation of the Isthmin recombinant eukaryotic expression vector, the encapsulation of the liposome of the Isthmin recombinant eukaryotic expression vector and the connection of the liposome encapsulated with the Isthmin recombinant eukaryotic expression vector and the antigen monoclonal antibody related to the anti-VIIIth factor. The immune liposome can actively target tumour neo-vascularization endothelial cells, release the Isthmin recombinant eukaryotic expression vector in the cells and express the Isthmin so as to play a role in anti-tumor angiogenesis. The invention has the advantages of effectiveness, low toxicity, simple preparation method and the like, and can be used for preparing medicaments for anti-tumor angiogenesis.

Description

technical field [0001] The invention relates to an immune liposome, in particular to an Isthmin gene-based immune liposome, and also relates to a preparation method and application of the immune liposome. Background technique [0002] Tumor is a malignant disease with high incidence and poor prognosis, which seriously threatens human health. After surgery, radiotherapy, and chemotherapy, biological therapy has become an important means in the comprehensive treatment of tumors. At present, tumor biological treatment strategies mainly include targeted therapy of tumor-related genes or tumor suppressor genes, immunotherapy of tumor-associated antigens, etc. There are certain limitations in practical applications. [0003] Studies have shown that more than 90% of all human tumors are solid tumors, and the growth and metastasis of solid tumors require the formation of new blood vessels. Inhibiting tumor angiogenesis can effectively block the nutritional support of tumor cells a...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/48A61K48/00A61P35/00A61P1/00A61K47/68A61K47/69
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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