Preparation method of microbial compound preparation based on various probiotic fermenting cultures
A compound preparation and fermentation culture technology, which is applied in the field of compound microbial preparations and microbial compound preparations, can solve the problems of unbalanced nutrient composition of the culture medium, affecting product quality and use effect, and improper selection of strains, so as to prevent and long-term control pests and diseases, Enhance the immunity of animal organisms, the effect of long shelf life of finished products
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Embodiment 1
[0040] 1. Preparation of culture medium
[0041] a) Medium for brewer's yeast fermentation
[0042] The medium for beer yeast fermentation contains the following raw materials in the following weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 200g, dipotassium hydrogen phosphate 200g, ferrous sulfate 8kg, manganese sulfate 900g, copper sulfate 300g, zinc sulfate 800g, boric acid 1.5kg, ammonium molybdate 50g and water 500kg;
[0043] b) A medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis The medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis comprises the following weight ratio Raw materials: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 200g, dipotassium hydrogen phosphate 200g, ferrous sulfate 8kg, manganese sulfate 900g, copper sulfate 300g, zinc sulfate 800g, boric acid 1.5kg, ammonium molybdate 50g and water 500kg;
[0...
Embodiment 2
[0054] 1. Preparation of culture medium
[0055] a) Medium for fermentation of aerobic bacteria (brewer's yeast)
[0056] The medium for beer yeast fermentation contains the following raw materials in the following weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 125g, dipotassium hydrogen phosphate 125g, ferrous sulfate 8kg, manganese sulfate 800g, copper sulfate 450g, zinc sulfate 900g, boric acid 1kg, ammonium molybdate 50g and water 500kg;
[0057] b) Medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis
[0058] The medium used for the fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis contains the raw materials of the following weight ratio: the medium used for the fermentation of beer yeast contains the following raw materials of the weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 125g, dipotassium hydrogen phosphate 12...
example 1
[0068] Example 1: Application effect test of probiotic microbial compound preparation on tomato
[0069] 1. Materials and methods
[0070] 1.1 Test material: probiotic microbial compound preparation; tomato variety: Fenruida.
[0071] 1.2 How to use: Dilute the probiotic microbial compound preparation 300 times with water for later use.
[0072] 1.3 Test method: The test adopts a block design, that is, the tomato is set every 67m 2 Apply 20kg of three-element compound fertilizer and add 0.1 (code name a), 0.2 (code name b), 0.3 (code name c), 0.4 (code name d), 0.5 (code name e) kg of probiotic microbial compound preparation respectively, and compound with single application of three elements Fertilizer was used as the control (ck), and a total of 6 treatments were set up, and each treatment was repeated 3 times. Tomatoes were sown on April 1, seedlings were raised in small sheds, and transplanted on May 20. The spacing between plants was 50cm and the row spacing was 52cm. ...
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