Preparation method of microbial compound preparation based on various probiotic fermenting cultures

A compound preparation and fermentation culture technology, which is applied in the field of compound microbial preparations and microbial compound preparations, can solve the problems of unbalanced nutrient composition of the culture medium, affecting product quality and use effect, and improper selection of strains, so as to prevent and long-term control pests and diseases, Enhance the immunity of animal organisms, the effect of long shelf life of finished products

Inactive Publication Date: 2010-08-18
孙世璋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microbial preparations on the market are single bacteria or simple compound bacteria preparations, so their functions are relatively single, and when used in planting and breeding industries, they can only improve one or several indicators of fertilizer or feed; In the preparation process of many compound bacteria preparations, some enterprises blindly think that the more types of bacteria added, the better, and put the mutually antagonistic bacteria together; Unbalanced or deviation in the setting of key parameters such as pH and temperature, resulting in problems such as low viable bacteria content, many bacteria, and high moisture content, which affects the quality of the product and its use effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of culture medium

[0041] a) Medium for brewer's yeast fermentation

[0042] The medium for beer yeast fermentation contains the following raw materials in the following weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 200g, dipotassium hydrogen phosphate 200g, ferrous sulfate 8kg, manganese sulfate 900g, copper sulfate 300g, zinc sulfate 800g, boric acid 1.5kg, ammonium molybdate 50g and water 500kg;

[0043] b) A medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis The medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis comprises the following weight ratio Raw materials: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 200g, dipotassium hydrogen phosphate 200g, ferrous sulfate 8kg, manganese sulfate 900g, copper sulfate 300g, zinc sulfate 800g, boric acid 1.5kg, ammonium molybdate 50g and water 500kg;

[0...

Embodiment 2

[0054] 1. Preparation of culture medium

[0055] a) Medium for fermentation of aerobic bacteria (brewer's yeast)

[0056] The medium for beer yeast fermentation contains the following raw materials in the following weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 125g, dipotassium hydrogen phosphate 125g, ferrous sulfate 8kg, manganese sulfate 800g, copper sulfate 450g, zinc sulfate 900g, boric acid 1kg, ammonium molybdate 50g and water 500kg;

[0057] b) Medium for fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis

[0058] The medium used for the fermentation of Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis contains the raw materials of the following weight ratio: the medium used for the fermentation of beer yeast contains the following raw materials of the weight ratio: honey 10kg, brown sugar 40kg, potassium dihydrogen phosphate 125g, dipotassium hydrogen phosphate 12...

example 1

[0068] Example 1: Application effect test of probiotic microbial compound preparation on tomato

[0069] 1. Materials and methods

[0070] 1.1 Test material: probiotic microbial compound preparation; tomato variety: Fenruida.

[0071] 1.2 How to use: Dilute the probiotic microbial compound preparation 300 times with water for later use.

[0072] 1.3 Test method: The test adopts a block design, that is, the tomato is set every 67m 2 Apply 20kg of three-element compound fertilizer and add 0.1 (code name a), 0.2 (code name b), 0.3 (code name c), 0.4 (code name d), 0.5 (code name e) kg of probiotic microbial compound preparation respectively, and compound with single application of three elements Fertilizer was used as the control (ck), and a total of 6 treatments were set up, and each treatment was repeated 3 times. Tomatoes were sown on April 1, seedlings were raised in small sheds, and transplanted on May 20. The spacing between plants was 50cm and the row spacing was 52cm. ...

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PUM

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Abstract

The invention relates to a preparation method of a microbial compound preparation based on various probiotic fermenting cultures. The various probiotics comprise Saccharomyces cerevisiae, Bacillus subtilis, Lactobacillus acidophilus, Rhodopseudomonas palustris and Enterococcus faecalis. The preparation method comprises preparation and fermentation processes of culture media. The preparation process of the culture media comprises the following steps of: (a) preparing a culture medium for fermenting the Saccharomyces cerevisiae; (b) preparing a culture medium for fermenting the Lactobacillus acidophilus, the Rhodopseudomonas palustris and the Enterococcus faecalis; and (c) preparing a culture medium for fermenting again after the Saccharomyces cerevisiae, the Lactobacillus acidophilus, the Rhodopseudomonas palustris and the Enterococcus faecalis are fermented and mixed and then the Bacillus subtilis strain is added for uniformly mixing. The fermentation process comprises the following steps of: (a) fermenting the Saccharomyces cerevisiae; (b) fermenting the Lactobacillus acidophilus, the Rhodopseudomonas palustris and the Enterococcus faecalis; and (c) fermenting the Bacillus subtilis. The invention has the advantages that the viable count in products is high, and the retention period of finished products is long.

Description

(1) Technical field [0001] The invention relates to a microbial compound preparation. Specifically, it relates to a compound microbial preparation based on a variety of probiotic fermentation cultures and its application. It belongs to the field of microbial preparations. (2) Background technology [0002] Due to the extensive use of pesticides, chemical fertilizers, antibiotics and hormones, the ecological environment and the quality of agricultural products have been increasingly seriously affected. For this reason, microbial agents need to be used to improve the ecological environment. At present, most of the microbial preparations on the market are single bacteria or simple compound bacteria preparations, so their functions are relatively single, and when used in planting and breeding industries, they can only improve one or several indicators of fertilizer or feed; In the preparation process of many compound bacteria preparations, some enterprises blindly think that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/18C12N1/20C12R1/865C12R1/125C12R1/23C12R1/38C12R1/01
Inventor 孙世璋袁歆
Owner 孙世璋
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