Double-wavelength absorbance detection device for analyzing trace liquid

A detection device and absorption photometry technology, which is applied in the field of microfluidic analysis of analytical chemistry, can solve problems such as very strict requirements on the incident angle of light, relatively large problems in interface coupling, and difficult fabrication of components, etc., to achieve integration and miniaturization. The effects of simplification, improvement of work reliability and simplification of instrument structure

Inactive Publication Date: 2010-08-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many technologies reported at present, and there are mainly the following two types that can effectively increase the optical path under the microsystem to the millimeter level: one is to use micromachining technology to make a high-reflectivity mirror inside the absorption pool (Noda, T. , Takao, H., Yoshioka, K., Oku, N., Ashiki, M., Sawada, K., Matsumoto, K., Ishida, M., Sensors and Actuators B-Chemical, 2006, 119: 245)( Steigert, J., Grumann, M., Brenner, T., Riegger, L., Harter, J., Zengerle, R., Ducree, J., Lab on a Chip, 2006, 6:1040), such techniques The longest optical path can reach 1 cm, but this technology has high requirements for micromachining, difficult parts production, and very strict requirements on the incident angle of light; another more advantageous technology is liquid core waveguide technology, the principle is to use low refractive index The material used to construct the detection cell, the difference between the measured solution and the detection cell material, thus using the principle of total reflection makes the light entering the liquid bound in the liquid and propagates along the waveguide axis, thereby obtaining a long optical path (Du, W.B.Qun , F.Fang, Z.L.Chemical Journal of C

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  • Double-wavelength absorbance detection device for analyzing trace liquid
  • Double-wavelength absorbance detection device for analyzing trace liquid
  • Double-wavelength absorbance detection device for analyzing trace liquid

Examples

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Example Embodiment

[0023] Example 1

[0024] see attached figure 1 2. The dual-wavelength ultraviolet absorption photometric detection device is composed of a first ultraviolet light emitting diode light source 1 and a second ultraviolet light emitting diode light source 2, a liquid core waveguide sample detection cell 3, a photodetector 4, and a control detection circuit 5. The four parts pass through the optical circuit and the circuit connection composition. The liquid-core waveguide sample detection cell 3 is composed of a transparent liquid-core waveguide containing the sample solution 10 to be tested. There are two turning areas on the liquid-core waveguide as the incident optical coupler 8 and the outgoing optical coupler 9 . The control and detection circuit 5 is connected to the light source through the control line 6, and lights the first ultraviolet light emitting diode light source 1 and the second ultraviolet light emitting diode light source 2 in a time-sharing manner. The light ...

Example Embodiment

[0026] Example 2

[0027] Example of absorbance analysis of a series of deoxyribonucleic acid (DNA) solutions using the apparatus of the present invention.

[0028] see figure 1 and figure 2 , the central wavelengths of the two ultraviolet light-emitting diodes as light sources in the device are 260 nanometers and 280 nanometers respectively. The incident angle of the two light-emitting diodes is 35°. A photodiode is used as the detector. The turning radius of the turning optical coupler is 0.3 mm, the radian of the turning part is 90°, and the distance between the two couplers, that is, the length of the sample detection cell, is 10 mm. The detection step is as follows: introduce the sample solution 10 to be tested into the sample detection cell 3, control the detection circuit 5 to light up the first light-emitting diode 1, turn off the second light-emitting diode 2, and the data acquisition module collects the transmittance output by the photodetector 4 at this time. ...

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Abstract

The invention relates to an ultraviolet double-wavelength absorbance detection device for trace liquid. The device is formed by connecting four parts which are a light source, a liquid core waveguide sample detection tank, a photoelectric detector and a control detection circuit by a light path and a line. The light source comprises two ultraviolet light emitting diodes with different wavelengths. The sample detection tank comprises a liquid core waveguide pipe, and the two pipe orifices of the liquid core waveguide pipe are opened and are used for leading in and leading out a solution to be analyzed. The liquid core waveguide pipe is provided with two turn areas used as an incident light coupler and an emergent light coupler of the liquid core waveguide sample detection tank. The control detection circuit lightens the two light emitting diodes of the light source in a time-sharing way, the intensity of light penetrating through the liquid core waveguide sample detection tank is respectively detected at each lightening moment by the detector, and the absorbance of the liquid to be detected under two wavelengths is obtained. The invention realizes the detection of the ultraviolet double-wavelength absorbance of the trace liquid and has high detection sensitivity of the device, little consumption quantity of a sample, simple and reliable detection operation, low manufacturing cost of the device and easy miniaturization and integration.

Description

technical field [0001] The invention relates to the microfluidic analysis field of analytical chemistry, in particular to an ultraviolet dual-wavelength absorption photometric analysis and detection device for trace liquids. Background technique [0002] The field involved in this patent is the detection system research in the field of analytical chemistry microfluidic analysis. The detection system is an important part of an analysis system. Since the microfluidic analysis system is based on microchannels, the reagent consumption is only picoliters or nanoliters, so the requirements for detection methods and devices are unique. [0003] Absorption spectrophotometry is a widely used detection method, which occupies an important position in analytical chemistry, and is also one of the earliest detection methods used in microanalysis systems. Its detection sensitivity is proportional to the optical path, but due to the The sample size of the system is small, and it is diffic...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 方群潘建章
Owner ZHEJIANG UNIV
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