Double-wavelength absorbance detection device for analyzing trace liquid
A detection device and absorption photometry technology, which is applied in the field of microfluidic analysis of analytical chemistry, can solve problems such as very strict requirements on the incident angle of light, relatively large problems in interface coupling, and difficult fabrication of components, etc., to achieve integration and miniaturization. The effects of simplification, improvement of work reliability and simplification of instrument structure
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[0023] Example 1
[0024] see attached figure 1 2. The dual-wavelength ultraviolet absorption photometric detection device is composed of a first ultraviolet light emitting diode light source 1 and a second ultraviolet light emitting diode light source 2, a liquid core waveguide sample detection cell 3, a photodetector 4, and a control detection circuit 5. The four parts pass through the optical circuit and the circuit connection composition. The liquid-core waveguide sample detection cell 3 is composed of a transparent liquid-core waveguide containing the sample solution 10 to be tested. There are two turning areas on the liquid-core waveguide as the incident optical coupler 8 and the outgoing optical coupler 9 . The control and detection circuit 5 is connected to the light source through the control line 6, and lights the first ultraviolet light emitting diode light source 1 and the second ultraviolet light emitting diode light source 2 in a time-sharing manner. The light ...
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[0026] Example 2
[0027] Example of absorbance analysis of a series of deoxyribonucleic acid (DNA) solutions using the apparatus of the present invention.
[0028] see figure 1 and figure 2 , the central wavelengths of the two ultraviolet light-emitting diodes as light sources in the device are 260 nanometers and 280 nanometers respectively. The incident angle of the two light-emitting diodes is 35°. A photodiode is used as the detector. The turning radius of the turning optical coupler is 0.3 mm, the radian of the turning part is 90°, and the distance between the two couplers, that is, the length of the sample detection cell, is 10 mm. The detection step is as follows: introduce the sample solution 10 to be tested into the sample detection cell 3, control the detection circuit 5 to light up the first light-emitting diode 1, turn off the second light-emitting diode 2, and the data acquisition module collects the transmittance output by the photodetector 4 at this time. ...
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