In-vitro rapid culture method for tender stem segments of blueberries

A cultivation method and tender stem technology, applied in the field of blueberry cultivation, can solve the problems of low reproduction rate, long cycle, easy to carry viruses, etc., and achieve the effect of high reproduction rate and fast reproduction speed

Inactive Publication Date: 2010-08-25
上海闵行区苗圃有限公司
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Blueberry is a high-quality and high-grade fruit with rich nutrition and high economic value. Its seedlings are mainly propagated by conventional method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro rapid culture method for tender stem segments of blueberries

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

Example 1

The composition of MS medium is as follows:

The 1 / 2Ms medium is prepared by halving the above-mentioned macro-elements and trace elements, and leaving other ingredients unchanged. The 1 / 4Ms medium is prepared by reducing the above-mentioned macroelements and trace elements to 1 / 4 of the above table, and the other ingredients remain unchanged. The second medium is based on 1 / 4Ms medium with Ms iron salt and 1 / 10Ms trace element, that is, the concentration of macroelement in the culture solution is 1 / 4 of the above table, and the concentration of trace element is in the above table The concentration of the two iron salt components is 2 times of the above table, and the other components remain unchanged.

The method for rapid in vitro cultivation of tender stems of blueberries, the specific steps are as follows:

Step 1: Select the semi-lignified tender stems of blueberries that germinated that year, cut off the leaves, rinse them, disinfect with 75vol% ethanol solution...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an in-vitro rapid culture method for tender stem segments of blueberries. The invention is characterized on that the method comprises the following steps: step one, selecting semi-lignified tender stems of the blueberries sprouted at the same year, cutting leaves, washing to be clean, and disinfecting; step two, cutting the disinfected tender stems of the blueberries to the tender stem segments for germination culture and cutting the new sprouts for inducing cluster buds; step three, cutting the cluster buds to simple buds for rooting culture; and step four, transferring test tube plantlets to a substrate formed by mixing perlite and peat based on a proportion of 5:1 by weight, transferring the seedling to a plastic potted tray filled with pure peat after 60 days and directly planting the seedling to a field after culture of two months. The invention has the advantages of high reproductive rate and rapid reproduction speed.

Description

technical field The invention relates to a rapid in vitro culture method for tender stems of blueberries. The semi-lignified tender stems of blueberries are used to induce clustered buds after being sterilized, and then complete test-tube seedlings are obtained after cultivation, which belongs to the blueberry cultivation method technology. field. Background technique Blueberry is a high-quality and high-grade fruit with rich nutrition and high economic value. Its seedlings are mainly propagated by conventional methods such as cuttings. The reproduction rate is low, the cycle is long and it is easy to carry viruses, which cannot meet the needs of production seedlings. Contents of the invention The purpose of the invention is to provide a method for cultivating blueberries with high reproduction rate and fast reproduction speed. In order to achieve the above object, the technical solution of the present invention is to provide a method for in vitro rapid cultivation of t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01G31/00
Inventor 陆锦明
Owner 上海闵行区苗圃有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap