In-vitro rapid culture method for tender stem segments of blueberries
A cultivation method and tender stem technology, applied in the field of blueberry cultivation, can solve the problems of low reproduction rate, long cycle, easy to carry viruses, etc., and achieve the effect of high reproduction rate and fast reproduction speed
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The composition of MS medium is as follows:
The 1 / 2Ms medium is prepared by halving the above-mentioned macro-elements and trace elements, and leaving other ingredients unchanged. The 1 / 4Ms medium is prepared by reducing the above-mentioned macroelements and trace elements to 1 / 4 of the above table, and the other ingredients remain unchanged. The second medium is based on 1 / 4Ms medium with Ms iron salt and 1 / 10Ms trace element, that is, the concentration of macroelement in the culture solution is 1 / 4 of the above table, and the concentration of trace element is in the above table The concentration of the two iron salt components is 2 times of the above table, and the other components remain unchanged.
The method for rapid in vitro cultivation of tender stems of blueberries, the specific steps are as follows:
Step 1: Select the semi-lignified tender stems of blueberries that germinated that year, cut off the leaves, rinse them, disinfect with 75vol% ethanol solution...
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