In-vitro rapid culture method for tender stem segments of blueberries

Abstract

The invention provides an in-vitro rapid culture method for tender stem segments of blueberries. The invention is characterized on that the method comprises the following steps: step one, selecting semi-lignified tender stems of the blueberries sprouted at the same year, cutting leaves, washing to be clean, and disinfecting; step two, cutting the disinfected tender stems of the blueberries to the tender stem segments for germination culture and cutting the new sprouts for inducing cluster buds; step three, cutting the cluster buds to simple buds for rooting culture; and step four, transferring test tube plantlets to a substrate formed by mixing perlite and peat based on a proportion of 5:1 by weight, transferring the seedling to a plastic potted tray filled with pure peat after 60 days and directly planting the seedling to a field after culture of two months. The invention has the advantages of high reproductive rate and rapid reproduction speed.

Description

technical field The invention relates to a rapid in vitro culture method for tender stems of blueberries. The semi-lignified tender stems of blueberries are used to induce clustered buds after being sterilized, and then complete test-tube seedlings are obtained after cultivation, which belongs to the blueberry cultivation method technology. field. Background technique Blueberry is a high-quality and high-grade fruit with rich nutrition and high economic value. Its seedlings are mainly propagated by conventional methods such as cuttings. The reproduction rate is low, the cycle is long and it is easy to carry viruses, which cannot meet the needs of production seedlings. Contents of the invention The purpose of the invention is to provide a method for cultivating blueberries with high reproduction rate and fast reproduction speed. In order to achieve the above object, the technical solution of the present invention is to provide a method for in vitro rapid cultivation of t...

AI Technical Summary

Problems solved by technology

Blueberry is a high-quality and high-grade fruit with rich nutrition and high economic value. Its seedlings are mainly propagated by conventional methods such as cuttings. The reproductive rate is low, the cycle is long and it is easy to carry viruses, which cannot meet the needs of production seedlings.

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