Method for preserving zostera marina seeds and zostera caespitosa seeds

A preservation method and a technology for the algae, applied in the seed and rhizome treatment, application, agriculture and other directions, can solve the problem of long-term preservation of algae and clump algae seeds, increased seed cumulative germination rate, influence on spring sowing, etc. problems, to restore the marine ecological environment, prolong the preservation time, high vitality and quality

Inactive Publication Date: 2010-09-01
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, under natural conditions, eelgrass and eelgrass seeds are not suitable for long-term preservation of eelgrass and eelgrass seeds, and directly affect the spring of the second year of eelgrass and eelgrass seeds sowing
According to the analysis, although the seeds of Z. chinensis and Z. clumps have dormancy characteristics in natural seawater at room temperature, they are not synchronized. Some seeds will gradually leave the dormancy state and enter the germination stage during the storage process, resulting in a gradual increase in the cumulative germination rate of seeds. the result of

Method used

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  • Method for preserving zostera marina seeds and zostera caespitosa seeds
  • Method for preserving zostera marina seeds and zostera caespitosa seeds
  • Method for preserving zostera marina seeds and zostera caespitosa seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] According to the above formula, 47.12 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 45 psu (that is, the weight ratio of artificial seawater to artificial seawater is 45‰). Every part of 300 eel algae seeds, get two parts and put them into two Erlenmeyer flasks containing artificial seawater with a salinity of 45psu respectively, place them at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12: 12. The light intensity is 20μmol m -2 the s -1 The purpose of inflating is to keep the seeds floating in the water without sinking to the bottom of the water. L:D=12:12 is the ratio of light time to dark time, that is, 12 hours of light and 12 hours of darkness, simulating day and night switching. Observe the status of the preserved seeds every week, and replace the artificial seawater with equal salinity every 5 to 7 days, so as to keep the seeds in a synchronized dormant st...

Embodiment 2

[0039] Similarly, 75.27 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 70 psu (that is, the weight ratio of artificial seawater to artificial seawater is 70‰). Every part of 300 eel algae seeds, get two parts and put it into two Erlenmeyer flasks that are filled with artificial seawater with a salinity of 70psu respectively, place them at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12: 12. The light intensity is 20μmol m -2 the s -1 Store in aerated environment under environmental conditions, replace with artificial seawater of equal salinity every 5 to 7 days, check the status of the seeds every month and count the germinated seeds for a total of 7 months. Table 4 shows the records of the cumulative germination rate of the seeds of Z. chinensis preserved at a salinity of 70 psu and a temperature of 4°C and 14°C.

[0040] Table 4

[0041] time

Cumulative g...

Embodiment 3

[0050] According to the above formula, 47.12 grams of artificial seawater is added to 1000 ml of pure water to prepare high-salinity artificial seawater with a salinity of 45 psu (that is, the weight ratio of artificial seawater to artificial seawater is 45‰). For each 300 grains of Z. clumpingensis seeds, two parts are put into two Erlenmeyer flasks filled with artificial seawater with a salinity of 45psu respectively, placed at a temperature of 4°C and 14°C respectively, and the photoperiod is D:L=12 : 12, the light intensity is 20μmol m -2 the s -1 The purpose of inflating is to keep the seeds floating in the water without sinking to the bottom of the water. L:D=12:12 is the ratio of light time to dark time, that is, 12 hours of light and 12 hours of darkness, simulating day and night switching. Observe the status of the preserved seeds every week, and replace the artificial seawater with equal salinity every 5 to 7 days, so as to keep the seeds in a synchronized dormant s...

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Abstract

The invention discloses a method for preserving zostera marina seeds and zostera caespitosa seeds, which comprises the following steps of: preparing artificial seawater with the salinity of 45psu to 70psu by using artificial seawater elements and pure water; directly putting the cleaned zostera marina seeds and zostera caespitosa seeds into a container filled with the artificial seawater; keeping the artificial seawater soaked with the seeds at the temperature of between 4 and 14 DEG C; and preserving the seeds in an environment with the light intensity of 20mu mol / m<-2>s<-1> and the photoperiod L:D=12:12, wherein the optimal holding temperature is 4 DEG C. The method can reduce the accumulated germination rate of the zostera marina seeds and zostera caespitosa seeds, prolong the preservation time of the seeds, and keep the higher vigor and quality of the seeds. By adopting the method of the invention, high-quality zostera marina seeds and zostera caespitosa seeds can be provided for artificial seeding, so the method is favorable for restoring and reconstructing sea grass lands, and provides a basic support for the quick and well restoration of marine ecological environment.

Description

technical field [0001] The invention relates to a method for preserving seeds, in particular to a method for preserving eosinopsis seeds and eosinopsis clump seeds. Background technique [0002] Both Zostera marina L. and Zostera caespitosa belong to the phylum Angiospermae, the class Monocotyledoneae (or Liliopsida), the order Alismales, and the eyes Potamogetonaceae (Potamogetonaceae), Zostera L., Zostera L. is a perennial herb with prostrate rhizomes, 2-4 mm in diameter, and elongated internodes. Each node has a first leaf and a Most fibrous roots. Erect stems tubular, laterally compressed, or sparsely branched, light green, membranous, internodes 30-35 mm long. Precursor leaves only sheathed without leaves, 2-5 mm long, membranous, translucent, closed casing, apex blunt, ventral side slightly sunken, with 3 veins; intrasheath small scales 2 or 4, linear; Leaf blade linear, green, alternate, up to 120 cm long, 2-12 mm wide, margin entire or wavy, rough, apex rounded; p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 潘金华江鑫李晓捷丛义周韩厚伟张壮志罗世菊姜广亮赵聚萍孙娟
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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