Method for detecting serum polypeptide for kidney transplantation rejection

A technology of rejection reaction and detection method, applied in the field of biomedical testing, can solve the problems of pain, high cost, unable to reflect the early changes of the transplanted kidney, etc.

Inactive Publication Date: 2012-12-19
眭维国
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Problems solved by technology

At present, the only internationally recognized method for diagnosing renal transplant rejection is transplanted kidney biopsy. This method is expensive, inconvenient to operate, and will cause trauma and pain to the patient. a series of complication
Some reports also indicate that this monitoring method does not reflect early changes in the transplanted kidney because renal function does not always correlate with histological improvement

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  • Method for detecting serum polypeptide for kidney transplantation rejection
  • Method for detecting serum polypeptide for kidney transplantation rejection

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Embodiment Construction

[0026] 1. Materials and methods

[0027] 1.1 Sample data

[0028] Serum samples were collected from fasting volunteers. 22 kidney transplant volunteers were sick volunteers who were proved to have rejection by renal biopsy, 12 sick volunteers with stable renal function after transplantation, and 13 normal outpatient physical examinations. 3ml of whole blood from volunteers was placed in a biochemical tube, and after standing at 4°C for 1h, centrifuged at 3000r / min for 5min, the supernatant was taken in an ice bath, filled in a portion of 20μl, and stored at -80°C.

[0029] Renal biopsy samples of rejection patients were classified according to the Banff97 classification method. Among them, 10 cases were diagnosed as acute rejection, and 12 cases were diagnosed as chronic rejection. Twelve cases of chronic allograft nephropathy occurred within 1.0 to 5.5 years after transplantation, and 10 cases of acute rejection occurred within 1.0 to 90.0 days after transplantation, all...

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Abstract

The invention discloses a method for detecting serum polypeptide for kidney transplantation rejection. The method comprises the following steps of: 1, classifying the previously collected serum samples according to the seriousness of the disease of a donor; 2, capturing the polypeptide from the serum in the serum sample groups by using MB-WCX respectively and concentrating the polypeptide on magnetic balls to form tie molecules; 3, eluting the tie molecules from the magnetic balls and performing MALDI-TOF mass spectrometric analysis; classifying the different peptide peaks measured from the samples of each group to establish a classifying and predicting model so as to acquire a difference peptide expression map; 4, combining the serum samples of a kidney transplantation rejection patient to be detected by using the MB-WCX, then performing the MALDI-TOF mass spectrometric analysis and comparing the analysis result with the map acquired in the step 3 so as to mark the peptide of the serum sample. The method contributes to understanding the pathogenesis for the kidney transplantation rejection well from the level of the proteomics and makes the early diagnosis of the kidney transplantation rejection possible.

Description

【Technical field】 [0001] The invention relates to the field of biomedical testing, in particular to a serum polypeptide detection method for renal transplantation rejection. 【Background technique】 [0002] Kidney allograft transplantation is the treatment of choice for most end-stage renal patients. With the development and progress of surgery and immunosuppressive therapy, the survival rate of patients who have survived for more than one year after transplantation has exceeded 90%, but the graft dysfunction that occurs early after transplantation is still one of the clinical problems that need to be solved urgently , need to be combined with the current treatments to deal with rejection for effective early monitoring. At present, the only internationally recognized method for diagnosing renal transplant rejection is transplanted kidney biopsy. This method is expensive, inconvenient to operate, and will cause trauma and pain to the patient. A series of complications such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N1/34G01N33/68
Inventor 眭维国戴勇黄礼玲晏强陈洁精
Owner 眭维国
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