Enzyme-linked immunosorbent assay sandwich method kit for detecting antigen of animal pestis F1
An enzyme-linked immunosorbent (ELISA) and F1 antigen technology, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problems of wrong test results and inability to obtain positive results, and achieves the reduction of reaction steps, the improvement of specificity, and the safety of the detection process. Effect
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Embodiment 1
[0027] Embodiment 1: Preparation of ELISA sandwich method kit for detecting animal plague antigen
[0028] 1. Preparation of ELISA sandwich method kit for detection of animal plague antigen
[0029] (1) The composition of the ELISA sandwich method kit for detecting animal plague antigens:
[0030] 1. Plague F1 antibody-coated plate, that is, F1Ab plate, 8×12 tube plate, 1 piece
[0031] 2. Horseradish peroxidase-labeled plague F1 antibody (HRP-F1Ab): freeze-dried 1 tube
[0032] 3. Glycerin. PB 2ml 1 stick
[0033] 4.10×PBS.T 20ml 1 bottle
[0034] 5. Plague F1 antigen positive control: 2ml 1 tube
[0035] 6. Negative control: 1 tube of 2ml
[0036] 7. Plague F1 antibody for inhibition: freeze-dried 1 tube
[0037] 8. TMB Chromogenic Solution: A liquid 6ml 1 bottle B liquid 6ml 1 bottle
[0038] 9. Chromogenic termination solution is 10% H 2 SO 4 : 6ml 1 bottle
[0039] 10.1% Thimerosal: 2ml 1 stick
[0040] (2) Preparation method of each component:
[0041] 1. P...
Embodiment 2
[0071] Embodiment 2: the application method of the ELISA sandwich method kit that detects animal plague antigen
[0072] 1. Preparation of reagents for detection of animal plague F1 antigen by ELISA sandwich method
[0073] 1. PBS.T: 10×PBS.T diluted 10 times with 0.85% NaCl.
[0074] 2. HRP-Fl antibody storage solution: add glycerol-PB2m1 to dissolve each HRP-Fl antibody (lyophilized agent), mix well, -20 0 C storage, shelf life of 6 months.
[0075] HRP-Fl antibody application solution: Dilute HRP-Fl antibody stock solution 30 times with PBS.T, 4 0 C kept for 10 days.
[0076] 3. F1 antigen positive control: Dissolve each F1 antigen positive control (lyophilized agent) with PBS.T2m1 and mix well.
[0077] 4. Negative control: Dissolve each negative control (lyophilized agent) in PBS.T2m1 and mix well.
[0078] 5. Plague F1 antibody for inhibition: dissolve and mix with PBS.T5m1.
[0079] 6. TMB Chromogenic Solution: Mix equal amounts of Chromogenic Solution A and B, an...
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