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Reaction vessel comprising conductive layer and inner non-metallic layer

A technology of reaction vessel and metal layer, which is used in laboratory containers, instruments, scientific instruments, etc.

Active Publication Date: 2013-08-07
RAPID DIAGNOSTICS ML LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is extremely difficult in rapidly heating and cooling systems

Method used

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  • Reaction vessel comprising conductive layer and inner non-metallic layer
  • Reaction vessel comprising conductive layer and inner non-metallic layer
  • Reaction vessel comprising conductive layer and inner non-metallic layer

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Embodiment Construction

[0104]figure 1 The reaction vessel shown is of the same general type as described eg in WO2005 / 019836 for use in equipment for performing PCR reactions.

[0105] The container comprises a plastic body 1 with an upper sample receiving part 2 with a relatively wide mouth enabling reagents to be easily added. In the embodiment shown, this upper part comprises a protruding flange 6 capable of interacting with a lifting arm in the device (as described in WO 2005 / 019836) allowing movement of the container in a device suitable for automatically performing the reaction.

[0106] At the lower end of the sample receiving part 2, the container ends in a capillary 3 which is sealed at the lower end by a transparent seal 4, forming a transparent window, thus forming an elongated reaction vessel capable of accommodating relatively small Sample 7.

[0107] An aluminum coating 5 surrounds the capillary 3 and is in intimate thermal contact with it. This coating 5 acts as a heat splitter capa...

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PUM

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Abstract

A reaction vessel for conducting a chemical or biochemical reaction, such as a polymerase chain reaction wherein at least one wall of said vessel comprises a metallic layer and an inner non-metallic layer. Reaction systems comprising combinations of vessels of the invention and apparatus for heating them, as well as particular reactions vessels are also described and claimed.

Description

technical field [0001] The present invention relates to reaction vessels for chemical and biochemical reactions that need to undergo controlled heating and / or cooling, and in particular to vessels that need to undergo thermal cycles in which a series of different temperatures are required. Background technique [0002] Specific examples of such reactions are the many nucleic acid amplification methods, especially the polymerase chain reaction (PCR). In this reaction, nucleic acid indexing is achieved by cycling a sample containing or suspected of containing a target nucleic acid through a repeated sequence of different temperatures in the presence of specially designed primer sequences and a polymerase capable of extending or expanding these primer sequences. formula amplification. These temperatures represent those necessary for nucleic acid denaturation (roughly requiring a temperature of about 95°C), primer annealing (at a lower temperature, eg, about 55°C), and primer e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01L7/00B01J19/00G01N21/03
CPCB01L7/52B01L2300/1883B01L2300/16G01N21/0332B01L3/50851B01L2300/0887B01L2300/1827B01L2300/0858
Inventor M·A·李D·J·斯奎雷尔R·P·琼斯R·J·威廉森G·R·格雷戈里G·古特塞尔
Owner RAPID DIAGNOSTICS ML LIMITED