Method for preparing food-grade acid pectase preparation
A kind of acid pectinase, food-grade technology, applied in the field of enzyme preparation production, can solve the problems of no growth, slow bacterial growth, bacterial pollution, etc., and achieve the effect of ensuring product quality and hygienic indicators
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Embodiment 1
[0019] A preparation method of a food-grade acid pectinase preparation, which comprises:
[0020] 1. Connect 500L of fermentation broth discharged from the fermentation tank with an initial temperature of 28°C, and pass cooling water to the jacket of the storage tank to cool it down to 16°C. Then add 0.5% by weight sodium dihydrogen phosphate and 0.5% by weight calcium chloride, and stand for 30 minutes to flocculate.
[0021] 2. Use 200 mesh powdered activated carbon as filter aid, prepare pre-coated suspension with pectinase solution and activated carbon, add activated carbon based on pectinase solution at w / v=0.5%, where W is in kg The unit of V is l; the steam is heated to 80°C for 10 minutes, and then pre-coated into the plate frame, the pure water is cooled down, and the effluent temperature is 20°C. Add activated carbon with w / v=0.5% based on the pectinase solution in the pectinase solution, where the unit of W is kg, and the unit of V is l; after mixing, filter the pectin ...
Embodiment 2
[0029] A preparation method of a food-grade acid pectinase preparation, which comprises:
[0030] 1. Connect 500L of fermentation broth discharged from the fermentation tank, with an initial temperature of 28°C, and pass cooling water to the jacket of the storage tank to cool it down to 20°C. Then, 1% by weight of sodium dihydrogen phosphate and 0.5% by weight of calcium chloride were added, and it was allowed to stand for 30 minutes for flocculation.
[0031] 2. Use 100 mesh powdered activated carbon as filter aid, prepare pre-coated suspension with pectinase solution and activated carbon, add activated carbon based on pectinase solution at w / v = 0.5%, where W is in kg The unit of V is l; the steam is heated to 80°C for 10 minutes, and then pre-coated into the plate frame, the pure water is cooled down, and the effluent temperature is 18°C. Add activated carbon with w / v=0.5% based on the pectinase solution in the pectinase solution, where the unit of W is kg, and the unit of V is...
Embodiment 3
[0039] A preparation method of a food-grade acid pectinase preparation, which comprises:
[0040] 1. Connect 500L of fermentation broth discharged from the fermentation tank, with an initial temperature of 28°C, pass cooling water to the jacket of the storage tank to cool down to 10°C. Then 1% by weight calcium chloride was added, and it was left to flocculate for 30 minutes.
[0041] 2. Use 150 mesh powdered activated carbon as filter aid, prepare pre-coated suspension with pectinase solution and activated carbon, add activated carbon based on pectinase solution at w / v = 0.5%, where W is in kg The unit of V is 1; the steam is heated to 80°C and maintained for 10min, and then pre-coated into the plate frame, the pure water is cooled down, and the effluent temperature is 18°C. Add activated carbon with w / v=0.5% based on the pectinase solution in the pectinase solution, where the unit of W is kg, and the unit of V is l; after mixing, filter the pectin with a pre-coated frame Enzyme...
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