Preparation method of pectinase preparation
A technology of pectinase preparation and pectinase, which is applied in the preparation of pectinase preparation, the preparation and canning of food grade acid pectinase liquid enzyme preparation, and can solve the problems of no growth, slow growth of bacteria, bacterial contamination, etc. problems, to achieve the effect of ensuring product quality and hygiene indicators
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Embodiment 1
[0019] A preparation method of pectinase preparation, which comprises:
[0020] 1. Connect 500L of fermentation liquid released from the fermentation tank, the initial temperature is 28°C, pass cooling water to the jacket of the storage tank to cool down to 16°C. Then add sodium dihydrogen phosphate with a weight percentage of 0.5% and calcium chloride with a weight percentage of 0.5%, and let stand for 30 minutes for flocculation.
[0021] Two, use 200 mesh powdered activated carbon as filter aid, add activated carbon with pectinase solution to prepare pre-coated suspension, add activated carbon by w / v=0.5% in terms of pectinase solution, wherein, the unit of W is kg , the unit of V is l; the temperature of the steam is raised to 80°C and maintained for 10 minutes, and then pre-coated into the plate frame, the temperature of the purified water is cooled, and the temperature of the effluent is 20°C. Add activated carbon with w / v=0.5% in the pectinase solution, wherein the uni...
Embodiment 2
[0029] A preparation method of pectinase preparation, which comprises:
[0030] 1. Connect 500L of fermentation liquid released from the fermentation tank, the initial temperature is 28°C, pass cooling water to the jacket of the storage tank to cool down to 20°C. Then add 1% by weight of sodium dihydrogen phosphate and 0.5% by weight of calcium chloride, and let stand for 30 minutes for flocculation.
[0031] Two, use 100 mesh powdery activated carbon as filter aid, add activated carbon with pectinase solution to prepare pre-coated suspension, add activated carbon by w / v=0.5% in terms of pectinase solution, wherein, the unit of W is kg , the unit of V is l; the temperature of the steam is raised to 80°C and maintained for 10 minutes, and then pre-coated into the plate frame, and the temperature of the effluent is 18°C. Add activated carbon with w / v=0.5% in the pectinase solution, wherein the unit of W is kg, and the unit of V is l; after mixing, filter the pectin with a pre-c...
Embodiment 3
[0039] A preparation method of pectinase preparation, which comprises:
[0040] 1. Connect 500L of fermented liquid released from the fermenter, the initial temperature is 28°C, pass cooling water to the jacket of the storage tank to cool down to 10°C. Then add calcium chloride with a weight percentage of 1%, and let stand for 30 minutes for flocculation.
[0041] Two, use 150 mesh powdery activated carbon as filter aid, add active carbon with pectinase liquid to prepare pre-coating suspension, add active carbon by w / v=0.5% in terms of pectinase liquid, wherein, the unit of W is kg , the unit of V is 1; the temperature of the steam is raised to 80°C and maintained for 10 minutes, and then pre-coated into the plate frame, the temperature of the purified water is cooled, and the temperature of the outflow liquid is 18°C. Add activated carbon with w / v=0.5% in the pectinase solution, wherein the unit of W is kg, and the unit of V is l; after mixing, filter the pectin with a pre-c...
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