Culture method of snow white mushroom strains and sporocarps

A technology of snow mushroom fungus and cultivation method, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of inability to cultivate fruit bodies, failure to explore medium formula, growth environment conditions or cultivation management methods, separation of No problems such as pure mycelium, to achieve the effect of excellent quality

Active Publication Date: 2010-11-03
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most wild edible fungi cannot be artificially cultivated, mainly because some are symbiotic fungi, and cannot complete their life cycle without the symbiotic environment, and cannot cultivate fruiting bodies. Conditions or cultivation management methods, some cannot separate pure mycelium, etc.

Method used

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  • Culture method of snow white mushroom strains and sporocarps
  • Culture method of snow white mushroom strains and sporocarps
  • Culture method of snow white mushroom strains and sporocarps

Examples

Experimental program
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specific Embodiment approach

[0022] The culture method of snow mushroom of the present invention (coprinus phalloides) bacterial classification and fruiting body, its preferred embodiment, comprises steps:

[0023] A. Isolation and cultivation of pure mycelium:

[0024] Collect wild uncapped snow mushroom fruiting bodies, sterilize them under aseptic conditions, put them on a special slant medium, cultivate them in a dark environment with a temperature of 25-28°C and a relative air humidity of <65% for 10-15 days, and obtain them after domestication. Snow mushroom mycelium;

[0025] B, carry out the cultivation of the special original species and production species of snow mushroom;

[0026] C, carry out the cultivation of snow mushroom fruiting bodies;

[0027] By repeating the above steps with the cultivated snow mushroom fruiting bodies, the cultivation of snow mushroom fruiting bodies can be repeated;

[0028] The white snow mushroom mycelium and white snow mushroom fruiting bodies are collected an...

specific Embodiment

[0114] Concrete embodiment, comprises steps:

[0115] 1. Isolation of wild strains

[0116] (1) Making a special culture medium

[0117] Raw materials and ratio: 100 grams of humus soil under the broad-leaved forest where wild mushrooms grow, 50 grams of soybeans (crushed into granules), 200 grams of peeled potatoes (cut into granules or slices of about 1 cubic centimeter), 20 grams of glucose, 2 grams of peptone, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, vitamin B13 tablets, 20 grams of agar.

[0118] Production of nutrient filtrate: Soak humus soil in 1500 ml of water for 5 minutes and stir constantly → filter with 4 layers of gauze to get the filtrate → boil soybean meal in water for 10 minutes → add peeled potatoes and cook for 10 minutes → filter with gauze to obtain nutrient filtrate.

[0119] Production of inclined test tubes: Add 20 grams of agar to the nutrient filtrate and boil to dissolve, add 20 grams of glucose, 2 grams of peptone, ...

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Abstract

The invention discloses a culture method of snow white mushroom (leucocoprinus cepaetipes) strains and sporocarps. The method comprises the following steps of: collecting wild unexpanded snow white mushroom entities, disinfecting under an aseptic condition, inoculating to a special slant medium, cultivating for 10-15 days at the temperature ranged from 25 to 28 DEG C and the relative air humidity less than 65 percent in a dark environment, and acclimating to obtain snow white mushroom mycelium, and cultivating special original seeds and production seeds of the snow white mushroom and cultivating snow white mushroom sporocarps. The snow white mushroom mycelium and the snow white mushroom sporocarps with the accession number of CCGMCCNo.1190 are preserved by China General Microbiological Culture Collection Center. The snow white mushrooms can be cultivated by manual work and has excellent quality and the potential of health care and medical efficacy.

Description

technical field [0001] The invention relates to a method for cultivating wild edible fungus strains and fruit bodies, in particular to a method for cultivating white snow mushroom strains and fruit bodies. Background technique [0002] More than half of the more than 10,000 species of wild macrofungi in the world have different economic values, and no less than 2,000 of them have important food and medicinal values. There are more than 1,000 kinds of edible fungi known in my country, more than 200 kinds can be cultivated experimentally, about 100 kinds can be cultivated economically, more than 50 kinds can be cultivated commercially, and more than 10 kinds can be cultivated industrially. Most wild fungi have not yet been cultivated. Successful domestication and cultivation. Finding out the artificial cultivation and domestication cultivation methods of edible and medicinal fungi with high edible value and medicinal value has always been one of the research hotspots of edible...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01H4/00C05G1/00
Inventor 潘新华何焕清
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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