Microbial strain capable of hydrolyzing 3-hydroxybutyronitrile

Abstract

The invention aims to provide a microbial strain arthrobacter nitroguajacolicus, slant medium, fermentation medium and application thereof. The 16S rDNA sequence of the strain is shown as SEQ ID No. 1. According to the formula, the slant medium comprises 0.2 to 2.0 weight percent of carbon source, 0.2 to 2.0 weight percent of nitrogen source, 0.01 to 0.5 weight percent of trace metal based on the amount of the metal salt, and 1.0 to 3.0 weight percent of agar powder, wherein the pH is 6.4 to 7.5. According to the formula, the fermentation medium comprises 0.5 to 3.0 weight percent of carbon source, 0.2 to 3.0 weight percent of nitrogen source, 0.01 to 0.5 weight percent of trace metal ion based on the amount of the metal salt, 0.01 to 0.1 weight percent of cysteine hydrochloride or cysteine and 0.01 to 0.1 weight percent of organic nitrile, wherein the pH is 6.4 to 7.5. The strain can be used for producing nitrilase; and the nitrilase produced by using the strain can catalyze 3-hydroxybutyronitrile so as to produce 3-hydroxybutyric acid. The 3-hydroxybutyric acid prepared by using the method has the advantages of cheap and readily available raw material, less hydrolysis byproduct, mild reaction condition and the like; and thus the microbial strain has high industrial production potential.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a microbial strain capable of hydrolyzing 3-hydroxybutyronitrile. Background technique [0002] 3-Hydroxybutyric acid, commonly known as β-hydroxybutyric acid, contains two optically active enantiomers, namely (R)-3-hydroxybutyric acid and (S)-3-hydroxybutyric acid, which are important pharmaceutical raw materials and Pharmacological reagent; Among them, (S)-(+)-3-hydroxybutyric acid is hyperlipidemia treatment drug (R)-4-hydroxy-3-hydroxybutyric acid [(R)-GABOB], a promoter of brain metabolism (S)-Oxiracetam (Oxiracetam), AIDS drugs, stabilizers and key intermediates of the latest generation of antibacterial drugs; and (R)-3-hydroxybutyric acid not only has nutritional functions, but also can treat diseases such as nervous disorders, Neurodegenerative diseases, insufficient oxygen supply, angina pectoris, myocardial infarction and glucose metabolism disorders and other...

AI Technical Summary

Problems solved by technology

However, the substrate ethyl acetoacetate in this method is insoluble in water, and the reaction cells or enzymes are easily inactivated in the organic phase, which limits the space-time yield of the product, and requires a stoichiometric amount of coenzyme during the reduction process.

[0009] These existing biological methods for producing 3-hydroxybutyric acid generally have shortcomings such as low yield, high cost, and high price, which have affected industrial research, development, and application promotion.

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