Method for purifying citrulline from watermelon

A technology of citrulline and watermelon, applied in chemical instruments and methods, preparation of organic compounds, preparation of urea derivatives, etc., can solve the problems of high energy consumption, low product content, poor impurity removal effect, etc., and achieve energy consumption The effect of low, high product content and short extraction time

Inactive Publication Date: 2010-11-10
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses an acoustic wave technique for extracting molecules from liquids quickly without causing them to be contaminated by other substances that may harm their properties or make it difficult to work on certain types of samples such as proteins. It also includes a special type called clarifying agents which help keep small particles suspended during processing while still allowing some larger ones to pass through easily. These improvements improve both efficiency and quality of purification processes.

Problems solved by technology

This patented technical problem addressed in this patents describes how to produce naturally occurrous flavors like taste buds containing various compounds including carboxylic acids found throughout their entire life cycle. Previous techniques involve producing purified forms of these molecules through physical means but they often result in contamination issues due to residual materials produced when processing them.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Remove the pulp from watermelon, take 5kg of watermelon peel and pulverize it into an ultrasonic extraction tank, add 15Lph1 hydrochloric acid aqueous solution and ultrasonically extract at a frequency of 80KHz for 1 hour, extract 3 times, combine the extracts, take 225ml of ZTC1+1 type sedimentation agent, add and stir evenly, precipitate, take the upper The supernatant was pumped into D3520 macroporous resin and 003×7 cationic resin series column for saturated adsorption, washed with deionized water, and 8BV ammonia water with a concentration of 0.1mol / L was taken to elute the cationic resin column, and the eluate was collected and recovered under reduced pressure to Density 1.5 place low temperature crystallization, centrifuge the crystallization (crystal content 61%), wash with deionized water and 90% ethanol in turn, dissolve in acid water with pH 1, adjust the isoelectric point of pH 6 with ammonia water to crystallize twice at low temperature, and then water the cr...

Embodiment 2

[0033] Remove watermelon pulp, take 5kg of watermelon rind and pulverize it into an ultrasonic extraction tank, add 30Lph2 sulfuric acid aqueous solution and ultrasonically extract at a frequency of 20KHz for 2 hours, extract twice, combine the extracts, take 1.8L101 juice clarifier, add sedimentation agent, stir evenly, precipitate, take Pump the supernatant into DM11 macroporous resin and D061 cationic resin series column for saturated adsorption, wash the neutral with deionized water, take 4BV ammonia water with a concentration of 1mol / L to elute the cationic resin column, collect the eluate, and recover it under reduced pressure to a density of 1.4 , place low-temperature crystallization, centrifuge the crystallization (58% crystallization content), wash with deionized water and 95% ethanol successively, dissolve the acidic water of ph3, and adjust the isoelectric point of pH6 with ammonia water to crystallize at low temperature for 3 times, and the crystallization is then w...

Embodiment 4

[0036] Remove the flesh from watermelon, take 5kg of watermelon rind and pulverize it into an ultrasonic extraction tank, add 15Lph1 citric acid aqueous solution and ultrasonically extract at a frequency of 60KHz for 1 hour, extract 3 times, combine the extracts, take 225ml of ZTC1+1 sedimentation agent, add and stir evenly, precipitate, take The supernatant was pumped into NK-II macroporous resin and 003×7 cationic resin series column for saturated adsorption, washed with deionized water, and 6BV of 0.2mol / L ammonia water was used to elute the cationic resin column, and the eluate was collected and deionized. Recover under pressure to a density of 1.5 and place low-temperature crystallization, centrifuge the crystallization (crystallization content 58%), wash with deionized water and 90% ethanol in turn, dissolve the acid water of ph3, adjust the isoelectric point of pH6 with ammonia water to crystallize twice, and the crystallization Wash with water and ethanol and dry to obt...

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Abstract

The invention relates to a method for purifying citrulline from a watermelon, which comprises the following steps: removing the pulp of a citrulline, carrying out ultrasonic extraction twice to three times with acid water, settling impurities with a settling agent, purifying citrulline with macroporous resin columns and strong-acid cation resin columns in series, eluting with ammonia water, carrying out concentrative crystallization, dissolving roughly crystallized substances with acid water, adjusting the pH with ammonia water, recrystallizing and the like. The method has the advantages of high extraction efficiency, low energy consumption and high product purity.

Description

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Claims

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Application Information

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Owner NANJING ZELANG AGRI DEV
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