Molecular marker of gene for regulating gel consistency of rice and application thereof

A technology of glue consistency and rice is applied in the field of rice glue consistency regulation gene molecular markers, which can solve the problem of less molecular markers and the like

Inactive Publication Date: 2010-11-10
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few molecular markers that can be directly applied to GC breeding so far. If the genetic variation of each gene that controls rice GC can be tr

Method used

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  • Molecular marker of gene for regulating gel consistency of rice and application thereof
  • Molecular marker of gene for regulating gel consistency of rice and application thereof
  • Molecular marker of gene for regulating gel consistency of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the design and application of Wx molecular marker

[0036] The 15 varieties of rice were obtained from the Rice Research Institute of the Chinese Academy of Agricultural Sciences. The rice gel consistency of 15 varieties of rice was detected by the method provided by the "Rice Quality Determination Standards of the Ministry of Agriculture of the People's Republic of China". After clustering, based on Wx (GENBANK ACCESSION NO.GQ151053), 15 rice varieties can be divided into 3 genotypes (see figure 1 -a). Type III is a cultivar of glutinous rice. After sequencing, it was found that there was a 23bp repeat sequence insertion in glutinous rice, which led to the early termination of the gene, which is why the GC value of glutinous rice is higher (the average value is 91.47). Type II cultivars had low GC values ​​(the average value was 39.55), and type I cultivars had moderate GC values ​​(the average value was 61.59). The gel consistency and Wx gene-based ge...

Embodiment 2

[0043] Embodiment 2, the design and application of AGPso molecular marker

[0044] After clustering, 15 rice varieties can be divided into five genotypes based on AGPiso genes (see figure 2 -a). Under the same Wx genotype background, type a had slightly higher GC value than other genotypes. The rice gel consistency and genotypes based on Wx and AGPiso genes of different rice varieties are shown in Table 3.

[0045] Table 3 Rice gel consistency of rice varieties based on Wx and AGPiso genotypes

[0046]

[0047]

[0048] Using the genomic DNA of rice as a template, carry out PCR amplification with the marker AGPisoM1, digest the PCR amplification product with restriction endonuclease BstXI, perform electrophoresis on the digested product and sequence the PCR amplification product (verify the electrophoresis result), The varieties of type b and type c obtained DNA fragments of 913bp, 536bp and 377bp, and the varieties of type a, type d and e obtained DNA fragments of 5...

Embodiment 3

[0051] Embodiment 3, the design and application of SBE3 molecular marker

[0052] After clustering, based on the SBE3 gene (GENBANK ACCESSION NO.GQ150913), 15 rice varieties can be divided into two genotypes (see image 3 -a). Type a includes 9 varieties including Nipponbare, type b includes 6 varieties including 93-11. Under the background of the same Wx genotype, the average GC of type a was slightly higher than that of type b. The rice gel consistency of different rice varieties and genotypes based on Wx and SBE3 genes are shown in Table 4.

[0053] Table 4 Rice gel consistency of rice varieties based on Wx and SBE3 genotypes

[0054]

[0055]

[0056] Genomic DNA of rice was used as a template, PCR amplification was carried out with the marker SBE3M1, and the PCR amplification product was subjected to electrophoresis and sequenced to verify the electrophoresis results. The type a variety obtained a 238bp DNA fragment, and the type b variety obtained a 210bp DNA fr...

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Abstract

The invention discloses a molecular marker of a gene for regulating gel consistency of rice and application thereof. The invention provides a method for identifying the rice with different gel consistency in an assisted manner, comprising the following steps: taking the genome DNA as a template and using WxM1 for PCR, determining the genetype of the rice as type I or type II if obtaining 252bp DNA and determining the genetype of the rice as type III if obtaining 275bp DNA; taking the genome DNA as a template, using WxM2 for PCR and Nhe I for enzyme digestion, determining the genetype of the rice as type I or type III if obtaining 1018bp DNA and determining the genetype of the rice as type II if obtaining 644bp and 374bp DNA; the gel consistency of the rice with genetype being type III being higher than that of the rice with genetype being type I and the gel consistency of the rice with genetype being type I being higher than that of the rice with genetype being type II; forming WxM1 by the DNAs shown in the sequences 1 and 2; and forming WxM2 by the DNAs shown in the sequences 3 and 4. The invention has important theoretical value and practice significance in rice quality improvement, has direct application value in purposefully improving the rice quality by utilizing the genetic engineering technology or using the molecular marker to assist breeding and has certain theoretical value in studying biosynthesis mechanism of plant starch.

Description

technical field [0001] The invention relates to a rice gel consistency regulation gene molecular marker and application thereof. Background technique [0002] Cultivating high-yield and high-quality new rice varieties has been the goal pursued by breeders for a long time. After decades of hard work, especially the use of hybrid rice technology, my country has achieved universally recognized achievements in rice production. However, in the past, how to solve people's food and clothing problems has always been the first priority, so the focus of rice breeding work is mostly concentrated on the cultivation of new high-yield varieties of rice, resulting in a relatively lagging behind in the breeding of high-quality rice, especially some high-yield varieties. The quality of hybrid rice generally deviates. [0003] One of the main reasons for the slow progress in rice quality improvement is its genetic complexity and the limitations of traditional research methods. The quality ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 李家洋顾铭洪钱前田志喜刘巧泉严长杰刘贵富王永红
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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