Method for constructing autoimmune recurrent abortion mouse model

A technology for recurrent miscarriage and construction methods, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of lack of autoimmune RSA animal models, number of implanted embryos, embryo loss rate and ACA drop In order to achieve good application prospects, reduce modeling time, improve the success rate and efficiency of modeling

Inactive Publication Date: 2010-12-01
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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Problems solved by technology

Looking at the existing mouse models of experimentally induced autoimmune RSA and APS, we found that although both can induce relevant clinical features, even if the same immunogen and immunization route are used, the results obtained such as implantation There are large differences in the number of embryos, embryo loss rate and ACA titer, which shows that there are certain defects in the experimental model induced by ACA
At present, there is also a lack of an ideal autoimmune RSA animal model, which brings great inconvenience to the study of the pathogenesis and treatment of the disease.

Method used

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  • Method for constructing autoimmune recurrent abortion mouse model
  • Method for constructing autoimmune recurrent abortion mouse model
  • Method for constructing autoimmune recurrent abortion mouse model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] 1. Materials and methods

[0010] 1.1 Reagents

[0011] Human β2GP-1 (β2-glycoprotein-1) was purchased from Cell Sciences, USA; CFA (Freund's Adjuvant Complete) and IFA (Freund's Adjuvant Incomplete) were purchased from Sigma, USA; BALB / c mice were purchased from China Provided by the Shanghai Experimental Animal Center of the Academy of Sciences.

[0012] 1.2 Experimental animals

[0013] 8-week-old clean-grade female and male BALB / c inbred mice, provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences, weighing 20±2g.

[0014] 2. Construction of animal models

[0015] 40 female Balb / c mice and 20 males. Females were randomly divided into normal pregnancy group (n=8), intrauterine injection of β2GP-1 group (n=8), intraperitoneal injection of β2GP-1 group (n=5), subcutaneous injection of β2GP-1 group (n=7), uterine Intrauterine injection of adjuvant group (n=7) and intrauterine injection of NS group (n=5).

[0016] The normal pregnanc...

Embodiment 2

[0040] 1. Materials and methods

[0041] 1.1 Reagents

[0042] Human β2GP-1 (β2-glycoprotein-1) was purchased from Cell Sciences, USA; CFA (Freund's Adjuvant Complete) and IFA (Freund's Adjuvant Incomplete) were purchased from Sigma, USA; BALB / c mice were purchased from China Provided by the Shanghai Experimental Animal Center of the Academy of Sciences.

[0043] 1.2 Experimental animals

[0044]8-week-old clean-grade female and male BALB / c inbred mice, provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences, weighing 20±2g.

[0045] 2. Construction of animal models

[0046] There were 37 female Balb / c mice and 19 males. Females were randomly divided into normal pregnancy group (n=7), intrauterine injection of β2GP-1 group (n=7), intraperitoneal injection of β2GP-1 group (n=5), subcutaneous injection of β2GP-1 group (n=7), uterine Intrauterine injection of adjuvant group (n=6) and intrauterine injection of NS group (n=5).

[0047] The norma...

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Abstract

The invention relates to a method for constructing an animal model, in particular to an animal experimental study model for pathogenesis study, diagnosis and treatment of autoimmune recurrent abortion and anti-phospholipid antibody syndrome. The autoimmune recurrent abortion mouse model is established by injecting human beta2GP-1 into a BALB / c mouse uterine cavity. The method for constructing the animal model is safe, reliable, simple and economic, reduces the modeling time of the autoimmune recurrent abortion (RSA) mouse model, improves the modeling success rate and modeling efficiency, and has good application prospect.

Description

technical field [0001] The invention relates to a method for constructing an animal model, which is used for animal experiment research on the pathogenesis research, diagnosis and treatment of autoimmune recurrent abortion and antiphospholipid antibody syndrome. Background technique [0002] Autoimmune recurrent spontaneous abortion (RSA) refers to autoimmune-related spontaneous abortions that occur three or more times in a row. It is an autoimmune disease and is considered to be an antiphospholipid antibody syndrome -phospholipids syndrome, one of the clinical manifestations of APS). For a long time, because the pathogenesis of autoimmune RSA and APS has not been elucidated, the treatment of autoimmune RSA and APS lacks clinically targeted and effective treatment measures, and patients often end up with re-pregnancy failure. The patient's family has caused great distress. Therefore, exploring the pathogenesis and treatment of autoimmune RSA is a hot research topic in rece...

Claims

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Application Information

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IPC IPC(8): A61K39/00
Inventor 赵爱民肖世金鲍世民
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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