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Influenza A virus medicament screening yeast two-hybrid system and application thereof

An influenza A virus and yeast two-hybrid technology, applied in the field of genetic engineering, can solve the problems of screening specificity, low screening throughput, unclear mechanism of action of target drugs, etc., and achieve the effect of high specificity

Inactive Publication Date: 2010-12-22
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a target-specific influenza A virus drug screening yeast two-hybrid system, to solve the existing technology for influenza A virus drug screening specificity, low screening throughput, unclear mechanism of action of the target drug and other technologies question

Method used

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  • Influenza A virus medicament screening yeast two-hybrid system and application thereof
  • Influenza A virus medicament screening yeast two-hybrid system and application thereof
  • Influenza A virus medicament screening yeast two-hybrid system and application thereof

Examples

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Embodiment 1

[0033] Example 1 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system using yeast two-hybrid plasmids pGADT7 and pGBKT7. The target-specific influenza A virus drug screening yeast two-hybrid system also includes The PB1 gene and PB2 gene of the international standard strain of influenza A virus A / PR8 / 34, the PB1 gene and pGBKT7 are recombined, and the PB2 gene and pGADT7 are recombined.

[0034] The DNA sequence complementary to the PB1 gene is shown in SEQ ID NO:1. In SEQ ID NO:1, the base sequence from position 25 to position 2298 corresponds to PB1 of the international standard strain of influenza A virus A / PR8 / 34 The protein coding region of the gene; the DNA sequence complementary to the base sequence of the PB2 gene is shown in SEQ ID NO: 2. In SEQ ID NO: 2, the base sequence from position 28 to position 2307 corresponds to Influenza A International The protein coding region of the PB2 gene of the standard strain A / PR8...

Embodiment 2

[0038] Example 2 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system, using yeast two-hybrid plasmids pGADT7 and pGBKT7, the target-specific influenza A virus drug screening yeast two-hybrid system also includes type A The PB1 gene and PA gene of the international standard influenza virus strain A / PR8 / 34, the PB1 gene and pGBKT7 are recombined, and the PA gene is recombined with pGADT7.

[0039] The DNA sequence complementary to the base sequence of the PB1 gene is shown in SEQ ID NO: 1; the DNA sequence complementary to the base sequence of the PA gene is shown in SEQ ID NO: 3, in SEQ ID NO: 3, The 25th to 2175th base sequence corresponds to the protein coding region of the PA gene of the international standard strain of influenza A virus A / PR8 / 34

[0040] The foreign gene sequence included in the target-specific influenza A virus drug screening yeast two-hybrid system is the PB1-pGBKT7 gene sequence of the international sta...

Embodiment 3

[0043] Example 3 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system. The target-specific influenza A virus drug screening yeast two-hybrid system includes the targets of Example 1 and Example 2. Site-specific influenza A virus drug screening yeast two-hybrid system.

[0044] Among them, the target-specific influenza A virus drug screening yeast two-hybrid system of Example 1 uses yeast two-hybrid plasmids pGADT7 and pGBKT7. The target-specific influenza A virus drug screening yeast two-hybrid system also includes influenza A The PB1 gene and PB2 gene of the virus international standard strain A / PR8 / 34, the PB1 gene and pGBKT7 are recombined, and the PB2 gene is recombined with pGADT7.

[0045] The target-specific influenza A virus drug screening yeast two-hybrid system of Example 2 uses yeast two-hybrid plasmids pGADT7 and pGBKT7. The target-specific influenza A virus drug screening yeast two-hybrid system also includes infl...

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Abstract

The invention is suitable for the technical field of genetic engineering, and provides a target specific influenza A virus medicament screening yeast two-hybrid system and application thereof. The target specific influenza A virus medicament screening yeast two-hybrid system comprises an influenza A virus PB1gene and an influenza A virus gene, a PB1 gene and a PA gene, and PB1, PB2 and PA genes. The target specific influenza A virus medicament screening yeast two-hybrid system for medicament screening has the advantages of high specificity, high throughput screening and exact mechanism of a target medicament.

Description

Technical field [0001] The invention belongs to the technical field of genetic engineering, and particularly relates to a yeast two-hybrid system, system and application for screening of target-specific influenza A virus drugs. Background technique [0002] Influenza viruses belong to the Orthomyxoviridae family (Orthomyxoviridae). They are enveloped negative-strand RNA viruses. The members include influenza A, B, and C influenza viruses. At present, influenza A and B viruses are the main infections in humans. The genomes of influenza A and B viruses contain 8 segments, which are single-stranded and negative-stranded RNAs, each encoding at least 10 proteins. According to the antigenic properties of hemagglutinin (HA) and neuraminidase (NA) on the surface of the virus, influenza A viruses can be divided into 1-16 HA subtypes and 1-9 NA subtypes. At present, the influenza viruses that cause human infection are mainly H1 (N1), H3 (N2) subtypes and type B influenza viruses. Influen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/44C12Q1/02
Inventor 房师松何建凡张仁利
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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