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Detection of curative effects of tamoxifen in auxiliary treatment of breast cancer

A tamoxifen and adjuvant therapy technology, applied in the fields of molecular biology and medicine, can solve problems such as loss of large fragments of genes, shifting of reading frames, etc.

Inactive Publication Date: 2010-12-29
HAINAN ZHUJIAN CELL MOLECULE GENETICS INSPECTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main way of gene mutation is that base deletion or substitution causes the reading frame to shift, or the loss of large fragments of genes

Method used

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Examples

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Embodiment Construction

[0022] Below in conjunction with specific embodiment, further illustrate the present invention. For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0023] The use of embodiment detection kit

[0024] Step 1: Extraction of DNA template

[0025] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0026] Step 2: Real-time quantitative PCR reaction

[0027] Use the fluorescent quantitative PCR kit in the detection kit to carry out the fluorescent quantitative PCR reaction. The reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, and 0.1 μl of 25 mM dNTP mixture solution, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense prim...

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Abstract

The invention discloses a kit for detecting curative effects of individual tamoxifen in auxiliary treatment of breast cancer. The kit comprises a specific primer pair and a specific fluorescent probe pair for detecting the polymorphic site of a target site CYP2D6 gene of tamoxifen administration, a quantitative polymerase chain reaction (PCR) conventional component and the like. The kit of the invention evaluates the curative effects of the individual tamoxifen in the auxiliary treatment of breast cancer by detecting the polymorphic site of the target site CYP2D6 gene of tamoxifen administration.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual tamoxifen adjuvant treatment of breast cancer, by detecting the polymorphism of the CYP2D6 gene at the site targeted by tamoxifen sites to assess the efficacy of individual adjuvant tamoxifen therapy in breast cancer. Background technique [0002] Breast cancer is a disease in which ductal epithelial cells of the mammary gland lose their normal characteristics and proliferate abnormally under the action of various internal and external carcinogenic factors, so that they exceed the limit of self-repair and become cancerous. The main clinical manifestation is breast lumps. Breast cancer is one of the most common malignant tumors in women. It has the characteristics of high incidence, aggressiveness, slow progression and long natural survival period. [0003] Breast cancer...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner HAINAN ZHUJIAN CELL MOLECULE GENETICS INSPECTION CENT
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