Compositions and methods to modulate cell membrane resealing

A technology of composition and cell membrane, applied in the field of polypeptide composition regulating cell membrane repair, can solve the problem that the nucleation process has not been completely solved

Active Publication Date: 2010-12-29
RUTGERS THE STATE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracellular calcium influx has been shown to be associated with fusion of intracellular vesicles with the plasma membrane, however, the molecular mechanisms involved in sensing damaged membrane signals and the nucleation process for repair sheet formation have not been fully understood solve

Method used

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  • Compositions and methods to modulate cell membrane resealing
  • Compositions and methods to modulate cell membrane resealing
  • Compositions and methods to modulate cell membrane resealing

Examples

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preparation example Construction

[0213] The preparation of monoclonal antibodies is well known in the art; see for example (Harlow et al., Antibodies: A Laboratory Manual, page 726 (Cold Spring Harbor Pub. 1988). Monoclonal antibodies can be prepared by injection with a composition comprising the antigen Mice or rabbits, serum samples taken to verify the presence of antibody production, spleen removed to obtain B lymphocytes, lymphocytes fused with myeloma cells to create hybridomas, hybridomas cloned, selection of clones that produce antibodies against antigens , and by isolating antibodies from hybridoma cultures. Monoclonal antibodies can be isolated and purified from hybridoma cultures by techniques well known in the art.

[0214] In other embodiments, antibodies can be produced recombinantly, such as by phage display or by combinatorial methods. Phage display and combinatorial methods can be used to isolate recombinant antibodies that bind to membrane repair polypeptides, MG53, membrane repair polypeptid...

Embodiment

[0239] Discovery of MG53, a muscle-specific TRIM family protein . MG53 was isolated using a previously established immunoproteomic approach that allowed the identification of proteins associated with myogenesis, Ca 2- Novel proteins involved in signaling and maintaining membrane integrity. Briefly, the method employs a monoclonal antibody library comprising ~6500 clones generated from mice immunized with triplet-rich membranes from rabbit skeletal muscle. Antibodies of interest were selected based on the z-line staining pattern of striated muscle sections observed under immunofluorescence microscopy. The target protein was purified by antibody affinity column, and the partial amino acid sequence of the purified protein was obtained. Based on the partial amino acid sequence, the complete cDNA encoding the target gene can be isolated from the skeletal muscle cDNA library. Homologous gene screening was then employed to search for the presence of different isoforms of the ide...

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Abstract

Disclosed herein are compositions and methods for repairing cell membranes. In addition, the invention relates to therapeutic compositions comprising nucleotides and / or polypeptides of the invention in combination with a pharmaceutically acceptable carrier, wherein the composition facilitates the repair of cell membranes. Moreover, the invention relates to the treatment and / or prevention of pathological conditions associated with cell membrane damage.

Description

[0001] References to related applications [0002] Under 35 U.S.C. §119(e), this application claims priority to U.S. Provisional Application No. 61 / 005,410, filed December 4, 2007, entitled Methods of Modulating Cell Membrane Resealing. [0003] combined by reference [0004] According to 37C.F.R. §1.52(e)(5), the sequence information contained in the electronic file name: Ma_2008utility_ST25.txt with a size of 57KB generated on December 2, 2008 using Patent-In 3.5 and Checker 4.4.0 is all The contents are hereby incorporated by reference. [0005] Statement Regarding Federally Sponsored Research [0006] The US Government has certain rights in this invention pursuant to the following grant awarded by the National Institutes of Health (NIH) to Dr. Jianjie Ma: RO1-HL0691000. technical field [0007] The invention relates to a polypeptide composition for regulating cell membrane repair and an application method thereof. Background technique [0008] To maintain cellul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K14/47C12N15/62A61K38/17
CPCC12N9/93C12N15/62C07K14/4716A61K38/17C07K14/47C07K19/00A61K38/53
Inventor 诺厄·魏斯勒德蔡传喜麻建杰
Owner RUTGERS THE STATE UNIV
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