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Plant bacteriostatic composition and application thereof

A plant antibacterial and composition technology, applied in plant growth regulators, botanical equipment and methods, applications, etc., can solve the problems of expensive preparation raw materials, numerous components, and complex preparation processes.

Active Publication Date: 2013-07-10
JIANCHANGBANG CHINESE HERBAL MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With more and more emphasis on product safety and environmental protection in recent years, a series of natural plant antibacterial agents have been developed, but these antibacterial agents are often formulated very complicated, requiring several or more than 10 ingredients, such as using field 7. The formulation of Codonopsis pilosula, honeysuckle, Coptis chinensis, Eclipta chinensis, Southern crane louse, Coix seed and other materials, so the preparation of raw materials is expensive, and the preparation process is complicated, and it is difficult to determine the quality of the final product due to the variety of ingredients

Method used

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  • Plant bacteriostatic composition and application thereof
  • Plant bacteriostatic composition and application thereof
  • Plant bacteriostatic composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 plant antibacterial lotion:

[0022] The following formulations were used:

[0023] The raw material formula of table 1 plant antibacterial lotion:

[0024] formula

Wild chrysanthemum (kg)

Sophora flavescens (kg)

Cnidium (kg)

1

20

10

10

2

20

15

10

3

20

10

15

4

20

5

5

5

20

15

15

6

20

5

15

7

20

15

5

[0025] Wild chrysanthemum, Sophora flavescens and Fructus Cnidii were purchased from the medicinal materials market in Haozhou, Anhui.

[0026] Wild chrysanthemum was distilled with water to obtain a volatile oil distillate for later use, and the residue was decocted for 1 hour. The decoction was filtered with filter paper, and the filtrate was purified through a macroporous resin adsorption column (specification LSA-33, purchase...

Embodiment 2

[0030] The quality inspection of embodiment 2 plant antibacterial lotions:

[0031] 1. Test method:

[0032] (1) Visually inspect the appearance.

[0033] (2) pH value: 10% sample aqueous solution was measured with a pH meter (Shanghai Precision Instrument Factory).

[0034] (3) Determination method of total flavonoids:

[0035] Get this product solution 10ml to 100ml volumetric flask, with 50% methanol constant volume, get this solution, rutin reference substance (source National Institute of Biological Products Inspection and Identification), 50% methanol 2.0ml respectively, put in 25ml volumetric flask, add water to 10ml, add 1ml 5% (weight / volume ratio) sodium nitrite solution, add 1ml 10% (weight / volume) aluminum nitrate solution, shake well and let stand for 5 minutes, add 5ml 1mol / L sodium hydroxide solution respectively, and water Constant volume, mix well, stand for 10 minutes, use a spectrophotometer (model UV-1240, Japan Shimadzu Corporation) at a wavelength of 5...

Embodiment 3

[0054] The bacteriostasis performance measurement of embodiment 3 plant antibacterial lotions

[0055] 1.1 Using the lotion prepared in Example 1, the test was carried out without sterilization.

[0056] 1.2 Activation of strains: Escherichia coli, Staphylococcus aureus, and Shigella flexneri (from Nanjing Medical University) fully rejuvenated in the slant medium were inoculated into the broth medium, and cultured at 37°C for 8 hours for later use.

[0057] Dilute the activated bacterial solution with sterile broth to a concentration equivalent to a 0.5 McFarland turbidity tube, i.e. 10 7 Cfu / ml. Add 1ml of bacterial solution to a sterile plate with a diameter of 9cm, then pour into M-H agar medium that has been melted and cooled to 55°C, and rotate to mix. After solidification, use a 6mm sterilized copper tube to punch 6 holes on the M-H agar, take out the medium in the copper tube with sterile tweezers, add 0.05ml of the drug solution to each hole, and incubate at 37°C for...

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Abstract

The invention discloses a plant bacteriostatic composition. The plant bacteriostatic composition comprises active ingredients and a pharmaceutically acceptable carrier, wherein the active ingredients consist of wild chrysanthemum, lightyellow sophora root and common cnidium fruit in a weight ratio of 2:0.5-1.5:0.5-1.5. The invention also discloses application of the plant bacteriostatic composition in preparation of a bacteriostatic agent and the quality standard thereof.

Description

technical field [0001] The invention relates to the field of antibacterial disinfectant, in particular to a plant antibacterial agent for resisting bacterial infection. Background technique [0002] Bacteriostatic agents are disinfection products. The antibacterial agents disclosed in the art are usually chemicals, such as using quaternary ammonium compounds as disinfectants, or proteins (composite enzymes), etc. Some of these antibacterial agents have great toxicity, or require harsh storage conditions. With more and more emphasis on product safety and environmental protection in recent years, a series of natural plant antibacterial agents have been developed, but these antibacterial agents are often formulated very complicated, requiring several or more than 10 ingredients, such as using field Seven, Codonopsis pilosula, honeysuckle, Coptis chinensis, Eclipta chinensis, Southern crane louse, coix seed and other materials are formulated, so the preparation of raw materials...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/20A01N65/12A01N65/10A01P1/00
Inventor 徐丽华潘国平吴洁梁春霞
Owner JIANCHANGBANG CHINESE HERBAL MEDICINE CO LTD
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