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Method for selecting and breeding Brassica napus recessive genic-male-sterile line through microspore culture technology and cloning technology

A technology of recessive genital sterility and microspore culture, applied in the field of Brassica napus breeding, can solve the problems of difficult breeding, long breeding cycle, heavy workload of testcross and testcross species identification, etc., and achieve the goal of breeding The effect of high efficiency and shortened breeding cycle

Inactive Publication Date: 2012-11-28
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, because the recessive GMS line is controlled by three pairs of genes (aabbRfRf), the segregation population is large, and the genotypes are many and complex, resulting in a large workload for testcross and testcross species identification, and great difficulty in breeding
Utilizing conventional breeding techniques, recessive genetic male sterile lines are applied in hybrid production in the form of recessive genetic sterile homozygous dimorphic lines, that is, recessive genetic male sterile lines need to be crossed by siblings of homozygous dimorphic lines Breeding and preservation ( figure 2 ), in order to make the genetic backgrounds of the sterile and fertile plants in the homozygous two-type line consistent, it must go through more than 5 generations of brother-sister crossing, which leads to a long breeding cycle, and it is necessary to produce all sterile lines in the female parent Pulling out 50% of the fertile plants in the homozygous two-type line will affect the yield and quality of the total sterile line

Method used

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  • Method for selecting and breeding Brassica napus recessive genic-male-sterile line through microspore culture technology and cloning technology
  • Method for selecting and breeding Brassica napus recessive genic-male-sterile line through microspore culture technology and cloning technology
  • Method for selecting and breeding Brassica napus recessive genic-male-sterile line through microspore culture technology and cloning technology

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Experimental program
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Embodiment 1

[0042] f 1 Configuration

[0043] The temporary insurance line M-6029, whose genotype is aabbrfrf, is the temporary insurance line of recessive genetic male sterile 20118A, which was passed consecutively in the test cross combination of recessive genetic male sterile 20118A by researcher Sun Chaocai of Shanghai Academy of Agricultural Sciences in 1999 Self-bred, test cross breeding, erucic acid content of 0.89%, glucosinolate content of 25.36μmol / g, excellent agronomic traits, high general combining ability and special combining ability.

[0044] Huyou 15, selected and bred by researcher Sun Chaocai of the Shanghai Academy of Agricultural Sciences, is the first Brassica napus variety in Shanghai that has passed the national rape variety approval and obtained the national plant new variety protection, and has been listed by the Ministry of Science and Technology and the Ministry of Agriculture It is one of the national-level key promotion varieties, with an oil content of 42.4...

Embodiment 2

[0047] Breeding of Recessive GMS Line DH203A

[0048] 1. Donor F 1 Microspore culture of material

[0049] During the flowering stage of rapeseed in March 2005, the donor material F 1 (Huyou 15×Linbao line M-6029) was cultured with microspores, and a total of 379 embryoid bodies were obtained, and 282 individual plants were obtained through induction culture, of which the doubled plants were 76 DH lines, which were identified by testcross , 11 DH strains were found to be of genotype aabbRfRf.

[0050] 2. Identification of DH recessive GMS lines

[0051] Because the main quality traits of rapeseed are female parent effects, the above 11 test crosses with the genotype of aabbRfRf sterile DH lines were used to test the quality traits, and a erucic acid content and glucosinolate content in line with the national double-low standard was obtained. The DH line with oil content ≥ 40% has better agronomic traits through field performance observation, so it was named as recessive ge...

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Abstract

The invention discloses a method for selecting and breeding Brassica napus recessive genic-male-sterile (GMS) line through the microspore culture technology and the cloning technology. The method comprises the following steps: a fine variety / line is hybridized with a recessive GMS line or temporary maintaining line to obtain the F1 generation; then the Brassica napus microspore culture technologyis adopted to perform inducing culture to the isolated microspores of the F1 generation plant and obtain a DH strain; and then the DH strain with the sterile character is test-crossed with the temporary maintaining line, 100% of the tester strain is sterile, the corresponding sterile DH strain is the recessive GMS line; and then the cloning technology is used to rapidly obtain a large number of plants of the sterile line which are used for the complete sterile line production. By using the method of the invention, the sterile line with stable and excellent genetic background can be rapidly obtained and used in the Brassica napus recessive GMS cross-breeding.

Description

technical field [0001] The invention relates to the field of Brassica napus breeding, in particular to a method for breeding a recessive male sterile line of Brassica napus by using microspore culture and asexual cloning techniques. Background technique [0002] The isolation technology of free microspores of Brassica napus is simple, and the efficiency of obtaining embryoid bodies and regenerated seedlings through free microspore culture technology is high, and microspore culture is an ideal method for studying the differentiation conditions of pollen cells, embryogenesis and morphogenesis mechanism. method, so this technology has been generally favored by rapeseed breeders in various countries. Over the years, many scholars have conducted extensive research on the composition of the medium for microspore culture, the size of buds, the temperature change during the microspore culture process, and the increase in the frequency of regenerated seedlings, making the microspore ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 孙超才杨立勇王伟荣周熙荣张智奇周音李延莉蒋美艳庄静顾龙弟
Owner SHANGHAI ACAD OF AGRI SCI