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Flow cell for electrochemiluminescence detection and system thereof

A technology of luminescence detection and flow cell, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reaction of materials, which can solve the problems of large volume and unfavorable miniaturization of instruments, and achieve the effect of avoiding interference

Inactive Publication Date: 2012-01-11
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Problems solved by technology

Such as the electrochemiluminescence immunoassay system of Roche Diagnostics (Elecsys 1010, Elecsys 2010 and E170 systems), but because the three-electrode system needs to be built in the flow cell, the volume is usually relatively large, which is not conducive to the miniaturization of the instrument

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  • Flow cell for electrochemiluminescence detection and system thereof
  • Flow cell for electrochemiluminescence detection and system thereof
  • Flow cell for electrochemiluminescence detection and system thereof

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Embodiment

[0054] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0055] as attached figure 1 , 2 As shown, the present invention discloses a flow cell for electrochemiluminescence detection and its system, which is used for the detection of electrochemiluminescent properties of fluorescent complexes, mainly by applying a certain potential on the electrode surface, so that the fluorescent complexes Oxidation-reduction reaction occurs on the surface of the electrode, and under the cooperation of co-reactants, an excited state is generated, and light is emitted when transitioning from the excited state to the ground state. By measuring the luminous intensity, the electrochemical luminescence performance of the complex is obtained.

[0056] In this implementation example, the flow-through electrochemiluminescent electrolytic cell consists of a base, a base block and a locking component. The base material is PEEK, on ​​which there i...

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Abstract

The invention discloses an iluminescence detection-based electrochemical flow cell and a system thereof. The flow cell mainly comprises a pedestal fixing an electrode chip, a base block with an electrolytic bath cavity and a locking part connecting and locking the pedestal and the base block, wherein a pipeline for a liquid flowing into and out of the electrolytic bath cavity, three conducting shrapnels contacted with three electrode output wires on the electrode chip respectively, and an optical window guaranteeing a sealing effect when an electrochemical luminous signal enters a light intensity detector are arranged on the base block with the electrolytic bath cavity. The whole detection system also comprises a peristaltic pump auxiliary providing flow power, a flow pipeline system and a signal receiving and processing system. The flow electrochemiluminescence detection system has the advantages of small volume and light weight, can be conveniently integrated in a portable small electrochemiluminescence detector, and can be directly used for electrochemiluminescence detection, electrochemiluminescence immunoassay or electrochemiluminescence nucleic acid hybridizing analysis, biochemical laboratories or hospital laboratory departments and the like.

Description

technical field [0001] The invention relates to an electrochemiluminescence detection device, in particular to a complex electroluminescence system based on a biophilin reaction that can be widely used in biological sciences, medicine, clinical and environmental sciences, and belongs to the field of detection equipment. Background technique [0002] Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by an electrochemical reaction on the surface of an electrode. Electrochemiluminescent immunodetection is achieved by detecting luminescent substances labeled on antibodies. [0003] Electrochemiluminescence detection does not require an external light source [Reference 1], which eliminates the characteristics of stray light and fluctuations caused by the instability of the light source in ordinary fluorescence analysis, thereby reducing noise and improving the signal-to-noise ratio. Sensitive photoelectric detection technology, this method has the advant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 方慧吕志成周明余林颇刘杨
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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