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Method for extracting and purifying polysaccharide compounds by utilizing ion-exchange fibers

A polysaccharide extraction and production method technology, which is applied in the field of ion exchange fiber extraction and purification of polysaccharide compounds, can solve the problems of large consumption of separation materials, poor monitoring of indicators, and low purity of polysaccharides, and achieve low operating costs and controllable quality standards , The effect of simple process

Inactive Publication Date: 2012-11-14
BEIJING INST OF CLOTHING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the efficiency of these methods is relatively low, the isolated polysaccharides have low purity, poor repeatability, many production links, and large consumption of separation materials, resulting in serious environmental pollution, high production costs, and long cycles.
Make the indicators in the separation process difficult to monitor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Wash, dry and pulverize Ginkgo biloba leaves, weigh 20g of powder, decoct with 200mL of water at 80°C for 2 hours, then decoct with 135mL of water for 2 hours, combine the decoction liquid and cool it, adjust the pH value of the solution to 5.0, and Flow rate 1.5BV h -1 , through a strongly basic ion-exchange fiber column with a weight of 1.0 g and a bed volume of 10 mL for adsorption. Then at a flow rate of 5.0BV·h -1 , 3 times column volume of water elution, and then 50% ethanol (50% ethanol: 3mol L -1 The volume ratio of hydrochloric acid (4:1) was eluted, and the ethanol polysaccharide extract was recovered, wherein the polysaccharide content was 61.08%, and the powder yield was 8.47%.

Embodiment 2

[0031] Wash, dry and pulverize the mulberry leaves, weigh 20g of powder, extract with 180mL of distilled water with pH=6 at 90°C for 3 hours, and decoct with 120mL of water for 2 hours. The yield of crude polysaccharide obtained was 25.60%, and the purity was 18.02%. The pH value of the upper column chemical solution is 4.0, and the flow rate is 2.0BV h -1 , the liquid concentration is 0.4mg·mL -1 Under the conditions of 1.0g and 10mL bed volume, the strong alkaline ion exchange fiber column is used for adsorption. The desorbent is 30% ethanol and 3mol L -1 Acetic acid mixture (volume ratio 4:1) 7BV, elution flow rate 5.0BV h -1 Perform elution. The ethanol was recovered to obtain a purified polysaccharide extract, wherein the polysaccharide content was 58.21%, and the powder extraction rate was 7.52%.

Embodiment 3

[0033] Weigh 250g of ginkgo leaf powder, decoct with 2300mL of distilled water at 85°C for 3 hours, then decoct with 1500mL of water for 2 hours, combine the decoction with a pH value of 7.3, and cool to obtain an extraction rate of 23.98%, a purity of It is 17.95% of the leached crude product. The pH value of the upper column chemical solution is 4.0, and the flow rate is 3BV h -1 , the polysaccharide concentration of the liquid medicine is 0.5 mg.m L -1 Under the conditions of 14.0g and 140mL bed volume, the strong basic ion exchange fiber column is used for adsorption. The desorbent is 40% ethanol and 3.0mol·L -1 Hydrochloric acid mixture (volume ratio 4:1) 8BV, elution flow rate 6BV h -1 Perform elution. The ethanol polysaccharide extract was recovered, wherein the polysaccharide content was 56.05%, and the powder extraction rate was 7.10%.

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Abstract

The invention relates to a method for extracting and purifying plant polysaccharide compounds by utilizing ion-exchange fibers. The method comprises the following concrete steps: cleaning leaves, stems, roots, fruits and other raw materials containing polysaccharide compounds, drying, pulverizing, decocting for extraction, and adsorbing with ion-exchange fiber columns under the following conditions: the stock solution concentration of polysaccharide compounds is 0.05-5.0 g.L<-1>, the temperature is 15-85 DEG C, the flow rate is 0.2-7.0B V.h-1 (BV: column volume), and the pH value is 2.0-10.0;eluting with an eluent under the eluting temperature of 10-90 DEG C at the flow rate of 0.2-12 BV.h-1, wherein the volume ratio of the eluent to a pH value regulator (the acid concentration is 0.4-6.0 mol.L<-1>) is 1-9:1; and collecting the eluent, and recycling the organic substances to obtain the polysaccharide compound extract powder. The method has the advantages of mature technique, accessible operating equipment, low operating cost, high operating efficiency and obvious energy-saving effect, obviously increases the product purity, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying natural plant medicinal components by using ion-exchange fibers, specifically, a method for extracting and purifying polysaccharide compounds by using ion-exchange fibers. Background technique [0002] Ion Exchange Fiber (IEF) is a fibrous ion exchange adsorption material, which is composed of many fiber monofilaments, and its diameter ranges from 20 to 300 μm. It itself contains fixed ions and mobile ions with the opposite sign to the fixed ions. When in contact with a solution capable of dissociating compounds, the active ions of the ion exchange fiber are exchanged with ions of the same sign in the solution, so it is called ion exchange fiber. Compared with granular ion exchangers, ion exchange fibers have the characteristics of large specific surface, small diameter, fast adsorption and desorption, large exchange capacity, easy regeneration, and various forms of use. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00B01D15/36
Inventor 刘廷岳聂素双崔成民
Owner BEIJING INST OF CLOTHING TECH