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Kit for detecting breast cancer susceptibility

A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to breast cancer, can solve problems such as unsatisfactory familial risk of breast cancer

Inactive Publication Date: 2011-03-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, BRCA1 and BRCA2 are not satisfactory in predicting the familial risk of breast cancer, because relatively few people carry BRCA1 and BRCA2 gene mutations

Method used

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  • Kit for detecting breast cancer susceptibility
  • Kit for detecting breast cancer susceptibility
  • Kit for detecting breast cancer susceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kit for detecting breast cancer susceptibility, its components and contents include: 1 μl 10×PCR reaction buffer, 0.5 μl 25mM MgCl 2 Solution, 0.2μl 25mM dNTP mixture, 0.2μl (5Units / μl) Taq DNA polymerase, 0.2ul each of 10μM specific primer pair (four), 0.5μl HaeIII endonuclease (10U / ul), 2μl 10× endonuclease Enzyme reaction buffer, deionized water 9μl. This kit is for one-person detection application, and the storage temperature of the kit is -20°C.

[0030] The use method of above-mentioned test kit comprises the following steps:

[0031] Step 1: Extraction of DNA template

[0032] Genomic DNA from peripheral blood was extracted using a blood genomic DNA extraction system (non-spin column type).

[0033] Step 2: PCR reaction, replication of the target fragment

[0034] Use a PCR detection kit for breast cancer susceptibility, which contains the following primers:

[0035] Sense primer 2: 5'-ACACACAATCCATAAAGTCACC-3'

[0036] (SED ID NO: 1) Tm value is 56°C

...

Embodiment 2

[0054] Through the method of Example 1, 261 cases of breast cancer patients and 356 cases of normal controls were genotyped, and the probability of each genotype corresponding to breast cancer patients and normal controls was analyzed, as shown in Table 1. We found that: Individuals carrying the FOXP3rs2294021CC genotype Compared with individuals carrying the FOXP3rs2294021TT genotype, the risk of developing breast cancer was 2.22 times higher, and individuals carrying the FOXP3rs2294021TC genotype were 1.43 times more likely to develop breast cancer than individuals carrying the FOXP3rs2294021TT genotype. Similarly, individuals carrying the CD 14rs2569190GG genotype have a 2.83-fold higher risk of developing breast cancer than individuals carrying the AA genotype, while individuals with the AG genotype have a 1.49-fold higher risk of developing breast cancer than individuals with the AA genotype (the above differences All are significant, P<0.05).

[0055] In addition, throug...

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PUM

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Abstract

The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting breast cancer susceptibility. The kit comprises specific primers used for detecting No. rs2294021 SNP locus on Foxp3 gene and No. rs2569190 SNP locus on CD14 gene, and a specific endonuclease used for detecting the two SNP loci. The estimation on the breast cancer susceptibility is realized by detecting polymorphism of the No. rs2294021 SNP locus on the Foxp3 gene and the No. rs2569190 SNP locus on the CD14 gene.

Description

technical field [0001] The invention relates to a kit for detecting susceptibility to a disease, in particular to a kit for detecting susceptibility to breast cancer, by simultaneously detecting the single nucleotide polymorphism (SNP) site of the Foxp3 gene and the single nucleotide polymorphism (SNP) site of the CD14 gene Nucleotide polymorphisms to predict individual susceptibility to breast cancer. Background technique [0002] Breast cancer is one of the common malignant tumors in women. The incidence of female breast cancer in my country is increasing year by year, accounting for 7% to 10% of malignant tumors. About 1.2 million women develop breast cancer throughout the year, accounting for 18% of female tumors. . [0003] Scientists have determined that mutations in the BRCA1 and BRCA2 genes greatly increase a woman's likelihood of developing breast cancer. Under normal conditions, these two genes convey information that controls cell reproduction. If they mutate, t...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 周翊峰郁晓邓杰琼王艳萍
Owner SUZHOU UNIV
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