Preparation method of haematococcus pluvialis extracts

A technology of Haematococcus pluvialis and its extract, which is applied in the field of preparation of Haematococcus pluvialls extract, can solve the problems of large consumption of chemical reagents, sensitivity and instability, and solvent residue, and achieve low cost and reliable quality. control, easy to operate

Active Publication Date: 2011-03-30
YUNNAN GREEN A BIOLOGICAL PROJECT
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Since astaxanthin oil is the main functional ingredient in Haematococcus pluvialis, it has strong antioxidant properties and is sensitive and unstable to light, heat, oxygen, and humidity. Therefore, the oil extraction process using common solvents can easily

Method used

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  • Preparation method of haematococcus pluvialis extracts
  • Preparation method of haematococcus pluvialis extracts
  • Preparation method of haematococcus pluvialis extracts

Examples

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Embodiment 1

[0031] Example 1 Preparation process of Haematococcus extract

[0032] This example optimizes the preparation process for the preparation of Haematococcus pluvialis extract by supercritical fluid extraction, specifically including the following steps:

[0033] 1) Broken wall of Haematococcus pluvialis

[0034] A. Wall breaking equipment

[0035] A high-pressure homogenizer is used for wall-breaking treatment.

[0036] B. According to the processing capacity and processing effect of the existing high-pressure homogenizer, the maximum processing capacity of algae mud at one time is 400-500kg (equivalent to 3000m 2 The total amount of algae sludge harvested by the culture system at one time).

[0037] C. The control parameters are shown in Table 1.

[0038] Table 1 Process control parameters of Haematococcus red spore wall breaking

[0039]

[0040] D. In addition to meeting the requirements of the above control parameters during the process of breaking the wall of Haem...

preparation example

[0058] Pack the broken Haematococcus pluvialis powder 500g into the extraction kettle of supercritical extraction equipment (HA121-50-02 type extraction device, Jiangsu Huaan Scientific Research Instrument Co., Ltd.), set the temperature and pressure of the extraction kettle and the separation kettle, and open equipment, open CO 2 The gas tank is used to heat and pressurize the extraction kettle at the same time. When the temperature reaches the extraction temperature of 70°C and the pressure reaches the extraction pressure of 45MPa, heat preservation and pressure are maintained while controlling the CO 2 Flow rate (15-25L / h), after extracting for 2-4 hours, receive a total of 125g of Haematococcus pluvialis extract from the outlet valve of the separation tank (50°C, 9MPa). It is determined that the mass percent content of astaxanthin in the Haematococcus pluvialis extract is 9.66%.

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Abstract

The invention provides a preparation method of haematococcus pluvialis extracts, a test method and application thereof. The preparation method of the haematococcus pluvialis extracts provided by the invention is a supercritical fluid extraction method using CO2 as extraction media. In the haematococcus pluvialis extracts prepared according to the method, the content of the astaxanthin can reach 2to 20 percent (w/w). The method provided by the invention has the characteristics of simple operation, low energy consumption, controllable quality and the like, the extracts also comprise various nutrient contents necessary for human bodies besides the functional ingredient of the astaxanthin, the haematococcus pluvialis extracts with high quality can be produced, and the raw material requirements required by medicine, health care food and ordinary food production can be met.

Description

technical field [0001] The invention relates to a preparation method of an extract of Haematococcus pluvialis, in particular to a method for preparing the extract of Haematococcus pluvialls by means of a supercritical fluid extraction method. Background technique [0002] Haematococcus is a single-celled green algae that can accumulate a large amount of astaxanthin under adverse conditions, and is recognized as the main source of natural astaxanthin in nature. Using supercritical fluid CO 2 The advanced Haematococcus pluvialis extract is not only rich in natural antioxidant astaxanthin, but also contains vitamins B1, B2, B6, B12, monounsaturated fatty acids, polyunsaturated fatty acids and other essential nutrients for human body. [0003] Astaxanthin is a red natural carotenoid, which has a powerful ability to scavenge oxygen free radicals. Its antioxidant activity is 550 times that of vitamins and 10 times that of carotenoids. It is known as a "super antioxidant" (1. Nak...

Claims

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Application Information

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IPC IPC(8): A61K36/05A23L1/30A61P9/00A61P17/16A61P25/00A61P27/02A61P35/00A61P37/04A61P39/06A23L33/10
Inventor 龙祥鸭乔胡志祥王朝军万庆家杜伟春张秋玲
Owner YUNNAN GREEN A BIOLOGICAL PROJECT
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