Three-dimensional optical tweezers system

Abstract

The invention provides a three-dimensional optical tweezers system. The system comprises a laser output unit, a spatial light modulator, a laser converging unit, a sample cell and a main observation unit, wherein the laser converging unit comprises a converging unit lens assembly and a front objective lens; the converging unit lens assembly is used for expanding each laser beam output by the spatial light modulator; the front objective lens is used for converging each expanded laser beam into the sample cell; the main observation unit comprises a visible light source, a rear objective lens and a main observation unit, namely a charge coupled device (CCD); the optical axes of the visible light source, the front objective lens, the rear objective lens and the main observation unit CCD are collinear to form a main optical axis; the sample cell is positioned between the front objective lens and the rear objective lens and is adjacent to both the front objective lens and the rear objective lens; both the front objective lens and the rear objective lens can move along the main optical axis; and the visible light source is positioned on the main optical axis of the front objective lens. By the system, a three-dimensionally distributed light trap can be observed under the condition that one surface of the sample cell is non-transparent. The system is particularly suitable for observation and operation of a biological living sample.

Description

technical field [0001] The invention relates to the field of optical technology, in particular, the invention relates to a three-dimensional optical tweezers system. Background technique [0002] An optical tweezers system is an optical system that uses optical traps to move tiny particles. Among them, the optical trap is formed by strongly converging the laser light with a high numerical aperture objective lens. The more concentrated the laser light is, the greater the gradient force of the optical trap is, which is more conducive to trapping tiny particles. The optical trap formed by the parallel laser incident parallel to the optical axis of the objective lens is in the center of the front focal plane of the objective lens; the parallel laser incident with a certain angle to the optical axis of the objective lens forms an optical trap at the place where the front focal plane of the objective lens deviates from the center; there is a certain divergence angle The light tra...

AI Technical Summary

Problems solved by technology

This design allows the CCD to only clearly observe the image of a specific focal plane formed by the convergence of parallel light beams by the objective lens, and it is difficult to make a global observation of the three-dimensionally distributed light traps

Moreover, since the optical tweezers system disclosed by Jennifer uses transmission imaging, the sample cell must be transparent on both sides, so the application range of the optical tweezers system is limited.

Images