Spirulina polysaccharide immune adjuvant and influenza vaccine containing the same

A technology of spirulina polysaccharide and immune adjuvant, which is applied in the direction of medical preparations containing active ingredients, antiviral agents, antibody medical components, etc., can solve the problem of limited supply of vaccine production, unavoidable side effects, slow vaccine onset time, etc. problems, to achieve the effect of weakened activity, obvious injection local swelling, and small side effects

Active Publication Date: 2011-04-27
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the current problems of single immune adjuvant, unavoidable side effects, limited supply of vaccine production, difficulty in coping with the large demand for vaccines when infectious diseases break out, and slow onset time of vaccines, the invention provides a vaccine that can improve the immune effect of vaccines and at the same time Spirulina polysaccharide immune adjuvant that brings huge economic and social benefits

Method used

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  • Spirulina polysaccharide immune adjuvant and influenza vaccine containing the same
  • Spirulina polysaccharide immune adjuvant and influenza vaccine containing the same
  • Spirulina polysaccharide immune adjuvant and influenza vaccine containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of nanoemulsion vehicle:

[0046] 1) Prepare materials according to the following mass ratio: 3g water for injection, 3g soybean oil for injection, 2g polyethylene glycol 400, 1.2g Tween80, 0.8g Span80;

[0047]2) Add 1.2g Tween80, 0.8g Span80, and 2g polyethylene glycol 400 into the mixing bottle, keep stirring for 1 to 10 minutes at a speed of 200 rpm at room temperature, mix well and add 3g injection Soybean oil, mixed well to obtain a mixture;

[0048] 3) Drop 3 g of water for injection into the mixture of the above 2), and at the same time, stir the mixture at a rotation speed of 200 rpm at room temperature until the liquid is transparent to obtain a nanoemulsion;

[0049] 4) The nanoemulsion prepared in 3) was tested as follows: 1. The particle size range was 28.985-56.493nm, which was qualified by the Malvern particle size detector; 3. No change in the nanoemulsion system after being placed at 4°C for 50 days, qualified.

Embodiment 2

[0051] 1) Weigh 10 mg of commercially available spirulina polysaccharide and dissolve it in 1000 μl of absolute ethanol to make a spirulina polysaccharide solution for later use;

[0052] 2) Mix the spirulina polysaccharide solution prepared in the above 1) with 1075 μl of the commercially available H3N2 influenza virus split vaccine (with a hemagglutinin content of 43.67 μg / ml), and then add 925 μl of normal saline to the mixture to obtain a total volume of 3000 μl of influenza vaccine containing spirulina polysaccharide, spare;

[0053] 3) Weigh 3g of soybean oil for injection, 2g of polyethylene glycol 400, 1.2g of Tween80, and 0.8g of Span80 in a stirring bottle, keep the rotation speed at 200 rpm, and stir at room temperature for 1 to 10 minutes until fully mixed to obtain mixture;

[0054] 4) Slowly drop 3000 μl of the influenza vaccine containing spirulina polysaccharide in 2) into the mixed solution of 3) above, while maintaining the rotation speed at 200 rpm, stir un...

Embodiment 3

[0057] 1) Weigh 7.6 mg of commercially available spirulina polysaccharide and dissolve it in 400 μl of absolute ethanol to obtain a spirulina polysaccharide solution for later use;

[0058] 2) Mix the spirulina polysaccharide solution in 1) with 3.4ml of commercially available H3N2 influenza virus split vaccine (hemagglutinin content 43.67μg / ml) to obtain 3.8ml influenza vaccine containing spirulina polysaccharide;

[0059] 3) Take 1.1g of polyoxyethylene castor oil, 1.6g of Span80, 6g of 1,2-propanediol, and 3g of isopropyl myristate in a stirring bottle, keep the rotation speed at 200 rpm, and stir at room temperature for 10 minutes until fully Mix well to obtain a mixed solution;

[0060] 4) Slowly drop 3.8ml of the influenza vaccine containing spirulina polysaccharide prepared in 2) into the mixed solution above 3), while maintaining the rotation speed at 200 rpm, stir until the solution is light yellow and transparent, and obtain spirulina polysaccharide, The nanoemulsio...

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Abstract

The invention provides a spirulina polysaccharide immune adjuvant and an influenza vaccine containing the same, wherein the spirulina polysaccharide immune adjuvant is prepared from the components in mass ratio as follows: 0.001-100% of spirulina polysaccharide and 0-99.999% of carrier, which is capable of preferably improving immunogenicity of the vaccine and greatly reducing dosage of antigen vaccine; the dosage of the antigen vaccine taking the spirulina polysaccharide as adjuvant is only a half of the dosage of the antigen vaccine taking the aluminum hydroxide as adjuvant or less, which can achieve ideal immune protection effect as well with high safety and small side effect; the influenza vaccine taking the spirulina polysaccharide as adjuvant is convenient for preparation and the quality thereof is easy to control.

Description

[0001] technical field [0002] The invention relates to a spirulina polysaccharide immune adjuvant and an influenza vaccine containing the spirulina polysaccharide immune adjuvant, belonging to the field of biotechnology. technical background [0003] Spirulina (Spirulina) is a nutrient-rich, widely distributed aquatic biological resource with great potential for development. In 1974, the United Nations Food and Agriculture Organization conference identified it as "one of the best protein sources" and "the most ideal food for human beings in the world". . Studies have shown that Spirulina polysaccharide (PSP) has the functions of promoting cell growth, improving body immunity, anti-tumor, anti-oxidation and anti-aging. research and development hotspots. [0004] Spirulina polysaccharide is extracted from spirulina by modern biochemical technology and process, and contains a variety of pure natural biological products with higher biological activity. It was developed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61P31/16
Inventor 李映波陈芹芹李慧高天荣
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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