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Microbacterium capable of utilizing methane and applications thereof

A technology of microbacteria and methane, applied in the field of microorganisms, can solve the problems that methane organisms cannot be engineered, and achieve the effect of high methane oxidation activity

Inactive Publication Date: 2012-07-04
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims at the inadequacies of the inability to engineer methane organisms in the prior art, and provides a microbacterium that can efficiently utilize methane. Its preservation number is CCTCC NO: M2010099, and its 16SrDNA sequence is shown in SEQ ID NO: 1

Method used

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  • Microbacterium capable of utilizing methane and applications thereof
  • Microbacterium capable of utilizing methane and applications thereof
  • Microbacterium capable of utilizing methane and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Purification and Identification of Microbacterium sp.DH

[0037] The bacterium is Gram-negative, and it spreads flat in the entire petri dish. The surface is smooth, showing a layer of yellow grease, and some have light yellow small colonies on it. The diameter of the colony is about 1mm, translucent, protruding, and the edge is smooth; Light yellow, yellow and orange, depending on the culture medium and culture conditions. Cell electron microscope scanning photo see figure 2 . The cells are short rod-shaped, with the center sunken inward, like a round basin, with an outer diameter of 0.6-0.7um and an inner diameter of 0.2-0.4um, which can produce fluorescent diffuse yellow pigment. The main physiological and biochemical characteristics of the strains are listed in Table 1.

[0038] Table 1 Physicochemical properties of Microbacterium sp.DH

[0039] Physiological and biochemical indicators feature Physiological and biochemical indicators f...

Embodiment 2

[0042] The growth curve of embodiment 2 strain Microbacterium sp.DH

[0043] By the growth curve of Microbacterium sp.DH strain (see image 3 ) shows that the delay period of Microbacterium sp.DH strain is longer, about 180h. However, when entering the logarithmic phase, the bacteria grew rapidly, and it took only 60 hours for the OD value to rise from 0.161 to 1.037, and then reached a plateau. The growth curve showed that the bacteria could obtain a larger concentration of bacteria with methane as the medium.

Embodiment 3

[0044] Example 3 Bacterial Strain Microbacterium sp.DH Utilizes Polycarbonate Proliferation Experiment

[0045] Use NMS medium to prepare 7 solutions of 3g / L glucose, fructose, mannose, lactose, sucrose, starch, and raffinose; take 20ml of the above 7 kinds of NMS medium and add them to a 100ml serum bottle. Take another serum bottle and add 20ml of NMS medium without adding carbon source as a control. Sterilize the above-mentioned medium by high-temperature steam at 121°C. After cooling, add 1ml of seed bacteria liquid into the bottle with a micropipette, cover the rubber stopper and shake well, put it in a shaker set at 30°C and 160 rpm / min conditions for 3 to 5 days. Then measure the OD of the bacterial solution 560nm Then take about 1.2ml of bacterial liquid and centrifuge at high speed, and then take the supernatant to detect the sugar concentration by the sulfate anthrone method to determine the consumption of carbon source. The results are shown in Table 2. All car...

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Abstract

The invention relates to a microbacterium capable of utilizing methane and the applications thereof. The CCTCC NO of the microbacterium is M2010099. The microbacterium can be used to prepare the methanogen absorbent, the garbage treatment agent and the biocatalyst. The microbacterium overcomes the application problems that the methane oxidizing bacteria have low microbial density, the cultivationof the bacteria is difficult to enlarged, etc; and the microbacterium has higher affinity to methane. The microbacterium is especially suitable for man-made methane sources such as domestic garbage landfills and rice fields and can be widely popularized.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a methane-utilizing microbacterium and its application. Background technique [0002] Methanotrophs are a special kind of methylotrophic microorganisms that use methane as the only carbon source and energy source. They have a wide distribution range. Methanotrophs are found in many environments such as acid, alkali, salt, high temperature, low temperature, and barren. exist. Its special metabolic process and metabolites have a significant impact on the global environment and have attracted widespread attention. Methanotrophs not only play an important role in global methane consumption, but also in the carbon, nitrogen, and oxygen cycles. [0003] Methanotrophs are Gram-negative bacteria that are strictly oligotrophic. Since methanotrophs were first isolated in 1906, eight genera of methanotrophs have been discovered so far, mainly including Methylomonas, Methylobacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01
CPCY02A50/20Y02P20/59
Inventor 赵天涛张丽杰赵由才全学军邓郁平
Owner CHONGQING UNIV OF TECH
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