Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof
A detection method, the technology of aquinetin, applied in the HPCE detection of aquinetin and aquinetin, in the fields of aquinetin and aquinone B, to achieve the effects of reduced analysis and operation costs, good correlation, and reduced environmental pollution
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[0030] 1 Instruments, reagents and samples
[0031] Agilent 3DCE capillary electrophoresis instrument (model: G1600A, including diode array detector and HP chromatography workstation); effective length of fused silica capillary 45cm (53.5cm×75μm ID, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.); American Waters 515 Type high performance liquid chromatograph; Waters 486 detector; 7725i manual sampler; pre-column: Phenomenex Security Guard; ChromTek chromatography workstation (American Sage Inc.).
[0032] Microfuge 18 desktop centrifuge (Beckman Coulter, USA); Boda 1004 ultrasonic cleaner (Shenzhen Boda Ultrasonic Engineering Equipment Co., Ltd.); TGL.16G desktop high-speed centrifuge (Shanghai Medical Analytical Instrument Factory); BP210D Type one hundred thousandth electronic analytical balance (Sartorius, Germany); Milli-Q ultrapure water device (Millipore Company, USA); filter device and microporous membrane (0.45μm).
[0033] Qin Pi A (110740-200104), Qin Pi Su (11172...
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