Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof

A detection method, the technology of aquinetin, applied in the HPCE detection of aquinetin and aquinetin, in the fields of aquinetin and aquinone B, to achieve the effects of reduced analysis and operation costs, good correlation, and reduced environmental pollution

Inactive Publication Date: 2013-05-01
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

Because there are many inconveniences in the analysis of the above four kinds of fennel coumarins by HPLC, the present invention provides a rapid method for detecting four active components in the fennel medicinal material or its extract by using HPCE, and provides a new means for the quality control of fennel

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  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof
  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof
  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof

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[0030] 1 Instruments, reagents and samples

[0031] Agilent 3DCE capillary electrophoresis instrument (model: G1600A, including diode array detector and HP chromatography workstation); effective length of fused silica capillary 45cm (53.5cm×75μm ID, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.); American Waters 515 Type high performance liquid chromatograph; Waters 486 detector; 7725i manual sampler; pre-column: Phenomenex Security Guard; ChromTek chromatography workstation (American Sage Inc.).

[0032] Microfuge 18 desktop centrifuge (Beckman Coulter, USA); Boda 1004 ultrasonic cleaner (Shenzhen Boda Ultrasonic Engineering Equipment Co., Ltd.); TGL.16G desktop high-speed centrifuge (Shanghai Medical Analytical Instrument Factory); BP210D Type one hundred thousandth electronic analytical balance (Sartorius, Germany); Milli-Q ultrapure water device (Millipore Company, USA); filter device and microporous membrane (0.45μm).

[0033] Qin Pi A (110740-200104), Qin Pi Su (11172...

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Abstract

The invention discloses a high performance capillary electrophoresis (HPCE) quick quantitative method for four active components of fraxin, esculin, fraxetin and aesculetin in cortex fraxini or extract thereof. By the method provided by the invention, four cortex fraxini coumarins are well separated and determined in 10min, and simultaneously all the components have good linear relations and highrecovery rates. The method provided by the invention has good relativity of determination results compared with a high performance liquid chromatography (HPLC) method, greatly shortens the analyzing time, obviously reduces environmental pollution, notably decreases the analyzing and operating cost, provides a quick efficient analyzing means for researching the distribution of the active components of cortex fraxini and the variation rule of content in different harvest phases, and also can be used for researching the inherent quality of the cortex fraxini extract and for referencing in development research of new drugs.

Description

Technical field [0001] The present invention relates to a method for detecting active ingredients in traditional Chinese medicines, in particular to a method for HPCE detection of Qin Pi A, Qin Pi B, Qin Pi B, and Qin Pi medicinal materials or extracts thereof. Background technique [0002] Qinpi varies from place to place, and the source of species is different, but all belong to the Oleaceae (Oleaceae) genus (Fraxinus) plants, and their morphological characteristics and smell are similar to those contained in the herbal medicine. The 2010 edition of the Pharmacopoeia of the People’s Republic of China stipulates that Qinpi is the dry branch of Fraxinus rhynchophylla Hance, Fraxinus chinensis Roxb., Fraxinus szabaona Lingelsh. or Fraxinus stylosa Lingelsh. Skin or dry skin. Among them, the ash tree is distributed in Jilin, Liaoning, Hebei, Henan and other places. Ash trees are distributed in North China, Northwest China, East China, Central South, Southwest and Liaoning. The s...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447A61K36/63A61K31/37A61K129/00
Inventor 张琦
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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