Thin-layer chromatography detection method of elecampane in fukant pills

A technique of thin-layer chromatography and a detection method, which is applied in the field of detecting ursolic acid in Muxiang by thin-layer chromatography, can solve the problems of unstable product quality and curative effect, and achieve the effects of being favorable for popularization, simple operation and low price.

Inactive Publication Date: 2011-06-29
HENAN KANGQI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Muxiang is an active ingredient in Fukang Pills. If it is not analyzed, it may lead to unstable product quality and curative effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The TLC detection method of woody fragrance in Fukang Pills, it operates as follows:

[0017] (1) Preparation of the reference substance solution: weigh 0.5 g of the reference medicinal material, add 15 ml of ethanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 1 ml of ether to dissolve the residue;

[0018] (2) Preparation of the test solution: Weigh 10 g of Fukang Pills, pulverize, add 40 ml of ether, heat to reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 1 ml of ether to dissolve the residue;

[0019] (3) Preparation of negative control solution: prepare Fukang Pills without woody fragrance by the same method according to the prescription amount of medicinal materials, and prepare negative control analysis solution according to step (2);

[0020] (4) Chromatographic identification: absorb the solution prepared in steps (1), (2), and (3) and place samples on a silica gel G thin-layer plate with sodium carboxyme...

Embodiment 2

[0022] The TLC detection method of woody fragrance in Fukang Pills, it operates as follows:

[0023] (1) Preparation of the reference substance solution: weigh 1 g of the reference medicinal material, add 30 ml of ethanol, heat and reflux for 40 minutes, filter, evaporate the filtrate to dryness, add 2 ml of ether to dissolve the residue;

[0024] (2) Preparation of the test solution: Weigh 10 g of Fukang Pills, pulverize, add 40 ml of ether, heat to reflux for 20 minutes, filter, evaporate the filtrate to dryness, add 2 ml of ether to dissolve the residue;

[0025] (3) Preparation of negative control solution: prepare Fukang Pills without woody fragrance by the same method according to the prescription amount of medicinal materials, and prepare negative control analysis solution according to step (2);

[0026] (4) Chromatographic identification: draw the solution prepared in steps (1), (2), and (3) and place a sample on a silica gel G thin-layer plate with sodium carboxymethy...

Embodiment 3

[0028] The TLC detection method of woody fragrance in Fukang Pills, it operates as follows:

[0029] (1) Preparation of the reference substance solution: weigh 1 g of the reference medicinal material, add 30 ml of ethanol, heat and reflux for 40 minutes, filter, evaporate the filtrate to dryness, add 1 ml of ether to dissolve the residue;

[0030] (2) Preparation of the test solution: Weigh 8 g of Fukang Pills, pulverize them, add 40 ml of ether, heat and reflux for 40 minutes, filter, evaporate the filtrate to dryness, add 2 ml of ether to dissolve the residue;

[0031] (3) Preparation of negative control solution: prepare Fukang Pills without woody fragrance by the same method according to the prescription amount of medicinal materials, and prepare negative control analysis solution according to step (2);

[0032] (4) Chromatographic identification: draw the solution prepared in steps (1), (2), and (3) and place a sample on a silica gel G thin-layer plate with sodium carboxy...

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PUM

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Abstract

The invention discloses a thin-layer chromatography detection method of elecampane in fukant pills, comprising the following steps: control sample solution preparation, test sample solution preparation and negative control solution preparation and chromatograph recognition: absorbing the solutions prepared in step (1), step (2) and step (3) on a silica gel plate for applying samples, and developing in the developer; spraying 5% vanillic aldehyde sulfuric acid solution, and heating at the temperature of 90-110 DEG C until spots develop; and carrying out comparative analysis on the same Rf values of the spots according to the test sample solution prepared in the step (2), the negative control solution prepared in the step (3), and the reference solution prepared in the step (1). The method provided by the invention has the beneficial effects of low price, strong feasibility and good repeatability, is simple to operate and is beneficial for popularization.

Description

technical field [0001] The invention belongs to the technical field of thin-layer chromatography detection, and in particular relates to a method for detecting ursolic acid in woody fragrance by using thin-layer chromatography. Background technique [0002] Muxiang is an active ingredient in Fukang Pills. If it is not analyzed, it may lead to unstable product quality and curative effect. [0003] The principle of using thin-layer chromatography to identify traditional Chinese medicine preparations is usually to add the test substance and the reference substance on the same thin-layer plate, develop it under the same conditions, and after the chromatographic spots are detected by color development, the obtained test substance and the reference substance are compared. The chromatograms of the products were compared and analyzed, so as to qualitatively identify the drugs. Thin-layer chromatography identification method has the characteristics of simplicity, rapidity, and easy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8905A61P15/00G01N30/90
Inventor 段桂生张艳红冯金河魏朝阳崔慧芳
Owner HENAN KANGQI PHARMA
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