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Method for preventing stroke by detecting folate dysbolism

A technique for folic acid metabolism, stroke, applied in the fields of molecular biology and medicine

Inactive Publication Date: 2011-06-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] Since there is no effective treatment for stroke at present, its prevention is particularly important, and effective primary prevention is the best way to reduce the burden of stroke

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] Example The use of detection kits

[0012] Step 1: Extraction of DNA template

[0013] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0014] Step 2: Real-time quantitative PCR reaction

[0015] Use the fluorescent quantitative PCR kit in the detection kit to carry out the fluorescent quantitative PCR reaction. The reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, and 0.1 μl of 25 mM dNTP mixture solution, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antisense primer, 0.25 μl each of 10 μM fluorescent probe with VIC and fluorescent probe with FAM, and 5.325 μl of deionized water.

[0016] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 50° C. for 2 minutes, 95° C. for 10 minutes, and 60 cycles of 95° C. for 3...

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Abstract

The invention discloses a method and a detection kit for preventing stroke by detecting folate dysbolism. Oral muomembranous cells of an examinee are collected, genomic DNA is extracted, the polymorphism of a 677C-T locus on a methylenetetrahydrofolate reductase gene (MTHFR) gene of the genomic gene is detected so as to determine whether folate dysbolism exists, and the examinee subjected to genic mutation is intervened through small dosage of folate and vitamin B in daily life, so that the stroke is prevented.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, and more specifically, the invention relates to a method for preventing stroke by detecting folic acid metabolism disorder. Background technique [0002] Statistics from the Ministry of Health of China in 2007 show that among the diseases that cause the death of the Chinese public, cerebrovascular disease ranks first, and stroke (commonly known as cerebral apoplexy) is the most common type of cerebrovascular disease. Stroke can be roughly divided into two categories, namely ischemic stroke (commonly known as cerebral infarction) and hemorrhagic stroke (commonly known as cerebral hemorrhage), and ischemic stroke accounts for 80% to 85% of all strokes. Stroke has become the leading cause of death and disability among middle-aged and elderly people. It is not only a tragedy for the patients themselves and their families, but also a heavy burden for the whole society. It is also a major...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨贺宪民
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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