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Endophytic fungus Penicillium ateckii from plant Chinese Umbrellaleaf rhizome and application thereof

A penicillium, epipodophyllotoxin technology, applied in fungi, microorganism-based methods, microorganisms, etc., can solve the problems of difficult artificial cultivation, complex chemical structure, long growth cycle, etc., to protect plant ecological diversity, The effect of simple fermentation conditions and short production cycle

Inactive Publication Date: 2012-05-23
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the complex growth environment of plants, it is difficult to simulate, and its growth cycle is long, it is difficult to carry out large-scale artificial cultivation, and tissue culture technology is not suitable for podophyllum plants
In addition, the chemical structure of podophyllotoxins is complex, although chemical synthesis can be carried out, but the cost is too high
The construction of engineering bacteria by means of genetic engineering is also impossible due to the large number of enzymes involved in the formation of such substances.

Method used

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  • Endophytic fungus Penicillium ateckii from plant Chinese Umbrellaleaf rhizome and application thereof
  • Endophytic fungus Penicillium ateckii from plant Chinese Umbrellaleaf rhizome and application thereof
  • Endophytic fungus Penicillium ateckii from plant Chinese Umbrellaleaf rhizome and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the separation and identification of Penicillium steeri WEQD

[0030] 1. Collection of samples

[0031] The samples were the roots and rhizomes of the plant Diphyllria sinensis L. collected from the Red River Valley (1560m above sea level) in Mei County, Shaanxi Province in June 2010.

[0032] 2. Isolation and Screening of Penicillium stezeri WEQD

[0033] The samples collected were washed with tap water respectively, processed with 75% (volume percentage) ethanol for 5min in the ultra-clean workbench, then rinsed with sterile water for 3-5 times; then with 2.5% (mass percentage) sodium hypochlorite Treat for 10 minutes, then rinse with sterile water 3-5 times. Use sterilized tweezers and razor blades to peel off the outer skin of the roots of Woerqi, cut them into small pieces of 0.5cm×0.5cm, plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days. At the same time, the roots of Woerqi treated with the same sterilization were not peeled...

Embodiment 2

[0039] Example 2. Preparation of epipodophyllotoxin diglucoside, astragaloside and rutin by using Penicillium stezeri WEQD

[0040] 1. Culture strains

[0041] Potato glucose liquid medium (natural pH): 200g peeled potatoes (cut into about 2cm 2 small pieces), put it into a beaker and boil for 30min, then filter it with double-layer gauze, take the filtrate and add 20g glucose, add water to make up to 1000mL.

[0042] Pick a single colony of Penicillium stutzeri WEQD and inoculate it in a 250ml Erlenmeyer flask (containing 100ml potato dextrose liquid medium, which has been sterilized), and cultivate it on a shaker at 28°C and 120r / min for 7 days to obtain a bacterial solution.

[0043] 2. Preparation of extract

[0044] 1. Filter the bacterial liquid twice with a suction filter, and collect the mycelium and fermentation liquid respectively.

[0045] 2. Extract the fermented liquid with an equal volume of chloroform (extractant), and get the extract;

[0046] 3, the raffinat...

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Abstract

The invention discloses an endophytic fungus Penicillium ateckii from plant Chinese Umbrellaleaf rhizome and application thereof. The invention provides a Penicillium ateckii WEQD CGMCC No. 3784. The Penicillium ateckii WEQD provided by the invention has the physiological property of producing epipodophyllotoxin diglucoside, astragaloside and rutin and can be used for preparing epipodophyllotoxinsubstances and flavonoid substances. The method for preparing epipodophyllotoxin diglucoside and / or astragaloside and / or rutin, provided by the invention, has the advantages of simple fermentation conditions, easy strain culture and short production period and has the application potential of industrial scale production. The invention provides a new approach for the resource development of epipodophyllotoxin substances and has important significance in protecting plant biodiversity.

Description

technical field [0001] The invention relates to an endophytic fungus Penicillium steerii from the plant Woerqi and its application. Background technique [0002] Podophyllotoxin (podophyllotoxin) mainly exists in the Berberidaceae Podophyllum and its related plants. It is a class of natural compounds with a wide range of biological activities, including estrogen-like, anti-estrogen-like, anti-cancer, anti-viral , antifungal, sedative, inhibiting plant growth and antioxidant effects. Podophyllotoxin has strong anticancer activity, and its mechanism of action is to inhibit the depolymerization of tubulin to prevent cell division and inhibit the activity of DNA topoisomerase. The glycoside derivatives of podophyllotoxin, etoposide and teniposide, have low toxicity and have good curative effects on various tumor diseases such as small cell lung cancer and lymphoma, so they have become broad-spectrum anti-tumor drugs for clinical application. [0003] At present, podophyllotoxi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/60C12P17/06C12R1/80
Inventor 赵长琦朱妍妍张嘉张正旺
Owner BEIJING NORMAL UNIVERSITY
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