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Method for improving water solubility of grape seed proanthocyanidin

A technology for proanthocyanidins and grape seeds is applied in the field of improving the water solubility of grape seed proanthocyanidins, and can solve the problems of complex process, poor water solubility and high production cost

Inactive Publication Date: 2011-08-31
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortage of specifications of grape seed extract on the market and the prior art, the technical problems to be solved by the present invention are: the defects of poor water solubility of the product, low oligomer content, complex process and high production cost

Method used

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  • Method for improving water solubility of grape seed proanthocyanidin
  • Method for improving water solubility of grape seed proanthocyanidin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Add 4 times the volume of deionized water to 100g of grape seeds, heat to 95°C, reflux for extraction for 4 hours, filter, and collect the extract;

[0017] 2. Add 2% pectinase by volume to the above extract, put in a water bath at 50°C, stir for 10 minutes, flocculent precipitates appear;

[0018] 3. Use an ultrafiltration membrane with a pore size of 0.8 μm to filter the above liquid to remove the precipitate;

[0019] 4. Concentrate and dry the above semen to obtain about 19.8 g of water-soluble grape seed proanthocyanidins.

Embodiment 2

[0021] 1. Add 5 times the volume of deionized water to 100g of grape seeds, heat to 85°C, reflux for 3 hours, filter, and collect the extract;

[0022] 2. Add 0.5% chitosan by volume to mass ratio to the above extract, put it in a water bath at 50°C, stir for 15 minutes, and flocculent precipitates appear;

[0023] 3. Use an ultrafiltration membrane with a pore size of 0.6 μm to filter the above liquid to remove the precipitate;

[0024] 4. Concentrate and dry the above clear liquid to obtain about 16.8 g of water-soluble grape seed proanthocyanidins.

Embodiment 3

[0026] 1. Add 4 times the volume of deionized water to 100g of grape seeds, heat to 95°C, reflux for extraction for 4 hours, filter, and collect the extract;

[0027] 2. Add 2% amylase by volume to the above extract, put in a water bath at 50°C, stir for 10 minutes, flocculent precipitates appear;

[0028] 3. Use an ultrafiltration membrane with a pore size of 0.4 μm to filter the above liquid to remove the precipitate;

[0029] 4. Concentrate and dry the above semen to obtain about 18.9 g of water-soluble grape seed proanthocyanidins.

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Abstract

The present invention discloses a method for improving the water solubility of grape seed proanthocyanidin. The method comprises the following contents of: carrying out reflux extraction of grape seeds by deionized water having a temperature of 85 to 95 DEG C, and filtering to obtain crude extract solution; adding pectase, amylase, chitosan, or high-purity ethanol into the crude extract solution and stirring slightly, and standing when flocky deposition occurs; ultrafiltrating supernatant liquor again; and concentrating and drying to obtain water soluble grape seed proanthocyanidin product.

Description

technical field [0001] The invention relates to a method for improving the water solubility of grape seed proanthocyanidins. Water-soluble grape seed proanthocyanidins are prepared by the methods of deionized water reflux extraction, extraction solution purification and ultrafiltration membrane separation, which belong to the field of extraction of natural active substances in biotechnology. Background technique [0002] Proanthocyanidins, the English name is Oligomeric Proantho Cyanidins (OPC), is a bioflavonoid with a special molecular structure, is currently internationally recognized as the most effective natural antioxidant for removing free radicals in the human body. It is generally a reddish-brown powder with a slight gas and astringent taste, soluble in water and most organic solvents. Typically grape seed extract or French maritime pine bark extract. Proanthocyanidin (grape seed extract) is a new type of high-efficiency antioxidant. It is the most powerful free r...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 刘丹田婷
Owner 天津市尖峰天然产物研究开发有限公司
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