Method for uniformly correcting excitation fluorescence intensity
A technology of excitation fluorescence and calibration method, applied in the fields of medical science, sensors, diagnostic recording/measurement, etc., can solve the problem that the relative concentration of fluorescent light source substances cannot be truly reflected.
Active Publication Date: 2012-11-21
BEIJING DIGITAL PRECISION MEDICAL TECH CO LTD
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[0006] In order to solve the technical problem that the fluorescence intensity distribution in the fluorescence image in the disclosed technology cannot truly reflect the relative concentration of the fluorescent light source substance in the biological tissue, the purpose of the present invention is to provide a uniform correction method for the excitation fluorescence intensity
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[0017] The method for removing autofluorescence interference of the present invention will be described in detail below with reference to the accompanying drawings. This method mainly comprises the following steps:
[0018] Step S1: as attached figure 1 As shown, the first excitation filter a and the second excitation filter b with the same spectral transmission curve (acceptable error within 5%) are customized at the optical element manufacturer. Place the first excitation filter a between the imaging object 1 and the excitation light source 2, place the second excitation filter b between the imaging object 1 and the CCD detector 3, and place the first excitation filter a on the excitation light source 2 exit, the excitation light emitted by the excitation light source 2 is filtered by the first excitation filter a, and then irradiates the surface of the imaging object 1, and the CCD detector 3 receives the excitation light emitted from the surface of the organism through th...
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The invention relates to a method for uniformly correcting excitation fluorescence intensity. According to the technical scheme, the method comprises the following steps of: putting one of two excitation optical filters with the same spectrum curve at an excitation light source outlet, putting the other excitation optical filter in front of a CCD (Charge Coupled Device) detector and illuminating an object with exciting light to obtain a first image by the detector; replacing the optical filter in front of the CCD detector with an emission optical filter and illuminating the object with exciting light to obtain a second image by the detector; dividing a pixel value in the second image by a corresponding pixel value in the first image to obtain an intermediate corrected image; and normalizing and rounding the intermediate corrected image to obtain a final corrected image. An error caused by the receiving of non-uniform excitation light intensity on the surface of an excited object is corrected by using an image of which the excitation fluorescence intensity is uniformly corrected, so that an excited fluorescence image can be reflected more truly.
Description
technical field [0001] The invention relates to a method for uniformly correcting the excited fluorescence intensity, which has a good application prospect in the excited fluorescence imaging technology. Background technique [0002] Molecular imaging is the application of imaging methods to conduct qualitative and quantitative research on biological processes in vivo at the cellular and molecular levels. Molecular imaging integrates the cutting-edge technologies of modern science such as molecular biochemistry, data processing, nanotechnology, and image processing, and has high specificity, high sensitivity, and ultra-high image resolution. Traditional medical imaging diagnosis shows the final effect of molecular changes, while molecular imaging focuses on physiological and pathological changes at the cellular or molecular level, which can not only improve the level of clinical diagnosis and treatment of diseases, but more importantly, it is expected to discover diseases at...
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IPC IPC(8): A61B5/00
Inventor 田捷薛贞文杨鑫秦承虎
Owner BEIJING DIGITAL PRECISION MEDICAL TECH CO LTD