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Microorganism composite zymocyte agent as well as preparation method and application thereof

A technology of composite fermentation bacteria and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of food waste deterioration and odor, reduce the protein content of food waste, etc., achieve growth inhibition, Good fresh-keeping effect and sensory quality, effect of increasing protein content and content of beneficial bacteria

Inactive Publication Date: 2012-10-24
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing fermentation strains used for food waste treatment use mold and yeast. The prepared protein feed has good palatability and is conducive to digestion. It has no obvious threat to pigs and humans, but because mold and yeast are suitable The pH value of growth is 6.5-7, which is conducive to the growth of spoilage bacteria and the reproduction of pathogenic microorganisms in food waste, which can easily lead to deterioration and odor of food waste, and at the same time reduce the protein content in food waste

Method used

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  • Microorganism composite zymocyte agent as well as preparation method and application thereof
  • Microorganism composite zymocyte agent as well as preparation method and application thereof

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Embodiment 1

[0021] The present invention is a kind of microbial compound fermentation agent, active component is Candida tropicalis (G.tropicalis) CGMCC NO.4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO.4202, Candida tropicalis liquid and Lactococcus lactis The bacterial liquid was mixed evenly according to the mass ratio of 1:1. Candida tropicalis was preserved in the China Microbial Culture Collection Management Center on September 30, 2010. The preservation number is CGMCCNO.4201, and the preservation address is Beichen, Chaoyang District, Beijing. No. 3, Yard No. 1, West Road, Institute of Microbiology, Chinese Academy of Sciences; Lactococcus lactis was preserved in the China Microorganism Culture Collection Management Center on September 30, 2010. The preservation number is CGMCC NO.4202, and the preservation address is Beijing No. 3, No. 1, Beichen West Road, Chaoyang District, Institute of Microbiology, Chinese Academy of Sciences, the preparation method is as follows: ...

Embodiment 2

[0030] The present invention is a kind of microbial composite fermentation agent, the active components are Candida tropicalis (G.tropicalis) CGMCC NO.4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO.4202, the strains of Candida tropicalis and Lactococcus lactis Mix evenly at a mass ratio of 0.5:1, and its preparation method is the same as in Example 1 above.

[0031] In this particular embodiment, the active component of the microbial compound fermentation inoculum also includes putrefaction Shewanella (Shewanella putrefacens), and the quality of this Shewanella putrefaces accounts for 25% of the compound fermentation inoculum, thereby being the bacterium of lactic acid bacteria and yeast Bacteria provide the carbon source necessary for growth.

Embodiment 3

[0033] The present invention is a kind of microbial composite fermentation agent, the active components are Candida tropicalis (G.tropicalis) CGMCC NO.4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO.4202, the strains of Candida tropicalis and Lactococcus lactis Mix evenly at a mass ratio of 2:1, and its preparation method is the same as that of Example 1 above.

[0034] In this particular embodiment, the active component of the microbial compound fermentation inoculum also includes putrefacci Shewanella (Shewanella putrefacens), and the quality of this Shewanella putrefaces accounts for 30% of the compound fermentation inoculum, thereby being a bacterium for lactic acid bacteria and yeast Bacteria provide the carbon source necessary for growth.

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Abstract

The invention relates to a microorganism composite zymocyte agent as well as a preparation method and an application thereof. The invention is characterized in that the active components of the composite zymocyte agent are Cida tropicalis and lactococcus lactis which are mixed according to the mass ratio of (0.5-2): 1. The preparation process comprises the following steps: carrying out oblique plane strain culture on the Cida tropicalis and lactococcus lactis; then carrying out liquid seed amplification culture to obtain a Cida tropicalis liquid and a lactococcus lactis liquid; mixing the Cida tropicalis liquid and lactococcus lactis liquid according to the mass ratio of (0.5-2): 1 and inoculating the mixture into a solid amplification culture medium to obtain the composite zymocyte agentaccording to the inoculum size of 4-5%(w / v); and adding the composite zymocyte agent to kitchen waste for microbial fermentation. The invention has the advantages of suppressing growth of putrefying bacteria in the kitchen waste and reproduction of pathogenic microorganism, thus featuring good freshness-retaining effect and organoleptic quality while increasing protein content and beneficial flora content of the kitchen waste.

Description

technical field [0001] The invention relates to a composite fermenting bacterial agent and a preparation method thereof, in particular to a microbial composite fermenting bacterial agent for treating kitchen waste, a preparation method and application thereof. Background technique [0002] Food waste refers to food waste and food residues in households, schools, government canteens, and catering industries, and is the main component of urban domestic waste. Food waste mainly includes rice and flour food residues, vegetables, vegetable oils, animal oils, etc. From the chemical composition, it includes starch, cellulose, protein, lipids, and inorganic salts. Its characteristics are as follows: 1. Crude protein and crude fiber High content of organic matter, such as high value of development and utilization, but perishable and produce stench; 2, high water content, inconvenient collection and transportation, improper treatment is likely to cause secondary pollution; 3, oil and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/16C12N1/20B09B3/00C12R1/74C12R1/46C12R1/01
CPCY02W30/82
Inventor 吴祖芳翁佩芳杨亚文
Owner NINGBO UNIV
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