Method for detecting influence of illumination intensity on energy conversion of photosynthesis indirectly

A technology of light intensity and photosynthesis, applied in the field of electrochemical detection, can solve problems such as short time and difficult research work

Inactive Publication Date: 2013-06-05
NORTHWEST NORMAL UNIVERSITY
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  • Description
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Problems solved by technology

However, due to the extremely short time for this reaction, it is at 10 -9 It is completed within seconds, so it brings great difficulties to the research work in this area

Method used

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  • Method for detecting influence of illumination intensity on energy conversion of photosynthesis indirectly
  • Method for detecting influence of illumination intensity on energy conversion of photosynthesis indirectly
  • Method for detecting influence of illumination intensity on energy conversion of photosynthesis indirectly

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Embodiment Construction

[0034] The water used in the experiment process was double distilled water, and the reagents used in the experiment were all analytically pure. The experiments were all carried out at room temperature.

[0035] Instruments and reagents used:

[0036] Electrochemical scanning microscope (SECM), CHI 900 electrochemical workstation (Austin Instruments, USA) were used for asymptote scanning experiments; quartz tube heating automatic double pure water still (1810B, Shanghai Asia Pacific Technology Glass Co., Ltd.) was used for distilling Secondary distilled water; electronic balance (Beijing Sartorius Instrument Co., Ltd.) for weighing medicines; ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); aluminum oxide grinding powder (0.05 μm, Shanghai Chenhua Instrument Reagent Co., Ltd.) For processing working electrode; Ag / AgCl is reference electrode (CHIⅢ, American CH Instrument Company); platinum is counter electrode; sodium chloride, sodium perchlorate, sodium dihydrogen...

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Abstract

The invention relates to a method for detecting the influence of illumination intensity on the energy conversion of photosynthesis indirectly, which comprises the following steps of: (1) processing a probe special for an electrochemical scanning microscope; (2) judging whether the processed probe can be used for experiments or not; (3) drying the processed probe; (4) performing circulating volt-ampere scanning and asymptote scanning on the dried probe to obtain a circulating volt-ampere curve of ferriporphyrin and an asymptotic curve of a two-phase reaction during 1 mM of benzoquinone phosphate buffered solution, and calculating driving force; (5) performing circulating volt-ampere scanning on the ferriporphyrin with different substituent groups, and calculating each driving force; (6) performing the asymptote scanning on the benzoquinone phosphate buffered solution at different molar concentrations; (7) performing the asymptote scanning on the benzoquinone phosphate buffered solutionwith different substituent groups and molar concentrations; (8) performing fitting on the obtained asymptotic lines, and calculating reaction speed constants; and (9) drawing the fitted asymptotic curve and a relational graph of the driving force and the reaction speed constants. The method is easy and convenient to operate quickly.

Description

technical field [0001] The invention relates to an electrochemical detection method, in particular to a method for indirect detection of the influence of light intensity on photosynthetic energy conversion. Background technique [0002] The primary reaction is the initial and key step in photosynthesis, and occupies an important and special position in photosynthesis. However, due to the extremely short time for this reaction, it is at 10 -9 It is completed within seconds, so it brings great difficulties to the research work in this area. It is currently believed that when light hits the leaves of green plants, the numerous "antenna pigment" molecules in chlorophyll (including most of chlorophyll a and all chlorophyll b, carotene and lutein) collect radio waves just like radio waves Like the antenna, it can receive light energy. The light energy received by these pigments can be transmitted to the "action center" extremely quickly and efficiently. "Action center" is a pi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416G01N27/48
Inventor 卢小泉孙瑞平乔志刚陕多亮孙萍王天夏
Owner NORTHWEST NORMAL UNIVERSITY
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