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Interference small molecule RNA of targeted LIN28 gene and glioma proliferation resistant application thereof

A glioma, small molecule technology, used in DNA/RNA fragments, gene therapy, antitumor drugs, etc., can solve problems such as unsatisfactory curative effect, high mortality rate, and easy recurrence.

Inactive Publication Date: 2011-09-14
上海生博生物医药科技有限公司
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  • Abstract
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Problems solved by technology

Because malignant glioma has the characteristics of rapid growth, strong invasiveness, easy recurrence after operation, and high mortality rate, although the comprehensive treatment level of glioma including surgical resection, radiotherapy and chemotherapy is constantly improving, its The overall effect is still not satisfactory

Method used

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  • Interference small molecule RNA of targeted LIN28 gene and glioma proliferation resistant application thereof
  • Interference small molecule RNA of targeted LIN28 gene and glioma proliferation resistant application thereof
  • Interference small molecule RNA of targeted LIN28 gene and glioma proliferation resistant application thereof

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Embodiment Construction

[0019] 1. Glioma cell culture: Glioma cell line U251 was cultured at 37°C, 5% CO 2 Constant temperature incubator. The cells in the logarithmic growth phase were digested with trypsin to remove the wall, then added an appropriate amount of culture medium and repeatedly pipetted to mix the cells; transfer the obtained cell suspension into a 15ml centrifuge tube, and centrifuge at 1500rpm for 3min; remove the supernatant; use 5ml sterile 1×PBS to suspend the cells, pipette and mix well; add about 1ml of cell suspension to a new culture bottle with 5ml of fresh medium, put in 37℃, 5%CO 2 Cultivate in an incubator, passage once in about 2 days (mainly check whether the cells are confluent in the culture flask).

[0020] 2. Separation of human glioma cells: Take human glioma surgical specimens and immerse them in KRBB solution (Krebs-Ringer bicarbonate buffer) at 4°C. Wash twice, then cut the specimens into small pieces, Transfer to a Erlenmeyer flask containing 5mL of digestive...

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Abstract

The invention relates to interference small molecule ribose nucleic acid (RNA) of a targeted LIN28 gene and glioma migration resistant application thereof. The interference small molecule RNA is in a base sequence shown as SEQ NO.1. In the invention, the interference small molecule RNA of the gene with the effect of inhibiting glioma proliferation is provided, a glioma cell model is used for researching the inhibiting effect of the interference small molecule RNA on the glioma proliferation, and a new method is provided for genetic therapy of glioma.

Description

technical field [0001] The invention relates to a small interfering RNA targeting LIN28 gene and its application in anti-glioma proliferation. Background technique [0002] Lin28 was first discovered in C. elegans as an abnormal gene controlling developmental time. A large number of studies have shown that Lin28 and Lin28B have extensive and important roles in the field of development and disease. In the process of human fibroblasts being induced into pluripotent stem cells, Lin28 is one of the four related induction factors, which play a regulatory role in the process of inducing differentiation. At the same time, it was found that the expression of Lin28 and Lin28B were activated in a variety of cancers, and their overexpression Expression often induces changes in cell shape. Studies have revealed that Lin28 plays an important biological role in embryonic cell biology, cell redifferentiation, development and cancer exploration. The study of Lin28 gene on the proliferati...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/10A61K48/00A61P35/00
Inventor 夏清梅车丽花殷珊黄红军陈国泽潘讴东
Owner 上海生博生物医药科技有限公司
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