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Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof

A technology of anti-cancer activity and unicornis, applied in the directions of organic active ingredients, medical preparations containing active ingredients, organic chemistry, etc., to achieve the effects of high extraction efficiency, good tumor-inhibiting activity, and high extraction selectivity

Inactive Publication Date: 2011-09-21
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a number of research results have shown that unicorn has anti-tumor activity, (GC-MS analysis and in vitro anti-tumor activity of unicorn supercritical extract [J]. Plant Research) but how to extract anti-cancer active ingredients efficiently and quickly There has been no conclusion, and it has been reported that the volatile components of unicorn lotus contain anticancer active substances such as β-sitosterol and campesterol, (Studies on the chemical components of unicorn lotus tuber volatile oil [J]. Journal of Jilin Agricultural University, 1996, 18 (2): 29-31.)

Method used

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  • Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof
  • Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 has the preparation of the unicorn supercritical fluid extract of anticancer activity

[0013] 180g unicorn root tuber dry powder is placed in 80% ethanol after soaking 3h, joins in the extraction axe, starts extraction, separates temperature control instrument and sets temperature and other parameters (comprising CO 2 Average flow 15kg·h -1 ). Start the refrigeration device to keep it within the range of 3-5°C. When the temperature reaches the set value, inject 100mL of 80% ethanol solution, and then properly adjust the valves to make the pressure in the extraction kettle meet the requirements. After the temperature in the extraction kettle, When the pressure reaches the set value range (extraction pressure / temperature 30MPa / 45°C, separation pressure / temperature 5MPa / 50°C), open the valve to allow the fluid to enter the separation kettle, and start timing at the same time, and inject the entrainer every 50min ( About 100mL 80% ethanol solution), inject t...

Embodiment 2

[0014] Gas chromatography and mass spectrometry analysis of embodiment 2 unicorn supercritical extract

[0015] Chromatographic conditions: DB-17MS capillary column (30m×0.25mm, film thickness 0.25μm), carrier gas is helium, vaporization chamber temperature 270°C, column flow rate 1mL min -1 , splitless injection, injection volume 1μl. Temperature program: keep the column temperature at 40°C for 4 minutes, increase the temperature at 10°C·min -1 Raise the temperature to 165°C, keep it for 15 minutes, and then increase the temperature at 5°C·min -1 Raise the temperature to 200°C, keep it for 2 minutes, and finally increase the temperature at 10°C·min -1 The temperature was raised to 250°C and kept for 10 minutes. The results of gas chromatography and mass spectrometry are shown in Table 1.

[0016] The gas chromatography and mass spectrometry analysis result of table 1 extract of the present invention

[0017]

[0018]

[0019] The extract of the present invention is...

Embodiment 3

[0020] Example 3 Inhibitory activity of unicorn lotus supercritical extract to cancer cells

[0021] Weigh appropriate amount of unicorn extracts from different methods and dissolve them in DMSO to prepare mother liquor, and then dilute with 2% maintenance solution to a concentration of 10mg·mL -1 , 2mg·mL -1 , 0.4mg·mL -1 , 0.08mg·mL -1 , 0.016mg·mL -1 , 0.0032mg·mL -1 , 0.0006mg·mL -1 , 0.0001mg·mL -1 . Colon cancer HCT-116, ovarian cancer HO-8910, gastric cancer SGC-7901, liver cancer BEL-7402 and liver cancer SMMC-7721 five human cancer cells in the logarithmic growth phase were collected and observed the next day after passage. After 80% of the culture flask was digested with trypsin, 100 μL of cell suspension per well was inoculated on a 96-well plate. at 37°C, 5% CO 2 After culturing in RPMI1640 medium for 24 hours, wash the 96-well plate with saline, 100 μL per well, shake it evenly and then suck it out. Then add in order of drug concentration from high to lo...

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Abstract

The invention discloses typhonium giganteum supercritical fluid extract with anticancer activity and a preparation method thereof. The extraction method is characterized in that the active ingredients of dry typhonium giganteum tuber powder serving as a raw material are extracted by using a supercritical fluid extraction technique and by regulating the parameters including flow of CO2, temperatures and pressures of an extraction kettle and a separation kettle and the like. The gas chromatography-mass spectrometer analysis on the extract indicates that the extract contains 40.22 percent of beta-sitosterol and 18.45 percent of campesterol. The results of microbiology and test technology (MTT) tests indicate that the typhonium giganteum extract obtained by the method has obvious tumor inhibition activity for cancer cell lines including colorectal cancer HCT-116, ovarian cancer HO-8910, gastric cancer SGC-7901, liver cancer BEL-7402 and liver cancer SMMC-7721. The extraction method has the advantages of high extraction selectivity, high extraction efficiency, high extraction speed, no pollution to extract, simplicity and convenience for operation, environment friendliness and the like. The extraction method disclosed by the invention can be used for extracting the anticancer active components in the typhonium giganteum and can be used an effective means for extracting sterols.

Description

Technical field [0001] The invention relates to a supercritical fluid extract of unicorn lotus tubers with anticancer activity and a preparation method thereof. The extraction method can be used for the extraction of anticancer active components in unicorn lotus tubers and the extraction of sterol substances. The main components are β-sitosterol and campesterol, especially the extract has high inhibitory activity on cancer cells, can be used in the development of potential anticancer drugs, and belongs to the field of medicine. Background technique [0002] The supercritical fluid extraction technology that emerged in the 1960s has been widely used in the fields of food, medicine, spices, petroleum, chemical industry, etc. , solvent extraction, etc.), supercritical fluid extraction has good separation selectivity, high extraction efficiency, good product purity and no organic solvent residue, complete biological activity of the product, simple process flow, low operating tem...

Claims

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Application Information

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IPC IPC(8): A61K36/888A61K31/575C07J9/00A61P35/00
CPCY02P20/54
Inventor 李庆勇祖元刚张宝友王春成宋瑱姜春菲
Owner NORTHEAST FORESTRY UNIVERSITY
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