Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof

A technology of anti-cancer activity and unicornis, applied in the directions of organic active ingredients, medical preparations containing active ingredients, organic chemistry, etc., to achieve the effects of high extraction efficiency, good tumor-inhibiting activity, and high extraction selectivity

Inactive Publication Date: 2011-09-21
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, a number of research results have shown that unicorn has anti-tumor activity, (GC-MS analysis and in vitro anti-tumor activity of unicorn supercritical extract [J]. Plant Research) but how to extract anti-cancer active ingredients efficiently and quickly There has been no conclu

Method used

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  • Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof
  • Typhonium giganteum supercritical extract with anticancer activity and preparation method thereof

Examples

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Example Embodiment

[0012] Example 1 Preparation of Unicornia unicornis supercritical fluid extract with anticancer activity

[0013] Put 180g of the dried tuber powder of unicornis in 80% ethanol after immersion for 3 hours, add it to the extraction axe, start the extraction, separate the temperature control instrument and set the temperature and other parameters (including CO 2 Average flow 15kg·h -1 ). Start the refrigeration device to keep it in the range of 3-5 ℃, and when the temperature reaches the set value, pour in 100 mL of 80% ethanol solution, and then adjust the valves appropriately to make the pressure in the extraction kettle meet the requirements. When the pressure reaches the set value range (extraction pressure / temperature 30MPa / 45°C, separation pressure / temperature 5MPa / 50°C), open the valve to let the fluid enter the separation kettle, and at the same time, start timing, and play the entrainer ( About 100mL of 80% ethanol solution), the fourth entrainer was injected and then...

Example Embodiment

[0014] Embodiment 2 Gas chromatography and mass spectrometry analysis of the supercritical extract of Unicornia japonica

[0015] Chromatographic conditions: DB-17MS capillary column (30m×0.25mm, film thickness 0.25μm), carrier gas is helium, vaporization chamber temperature is 270℃, column flow rate is 1mL·min -1 , splitless injection, injection volume 1μl. Program temperature: the column temperature was kept at 40 °C for 4 min, and the temperature was adjusted to 10 °C·min. -1 The rate of heating was increased to 165 °C, maintained for 15 min, and then increased to 5 °C·min. -1 The rate of heating was increased to 200 °C, maintained for 2 min, and finally at 10 °C min -1 The rate was ramped up to 250°C and held for 10 min. The results of gas chromatography and mass spectrometry analysis are shown in Table 1.

[0016] Table 1 Gas chromatography and mass spectrometry analysis results of the extract of the present invention

[0017]

[0018]

[0019] The extract of t...

Example Embodiment

[0020] Example 3 Inhibitory activity of unicornis supercritical extract on cancer cells

[0021] Weigh an appropriate amount of unicornis extract in different methods and dissolve them in DMSO to prepare a mother solution, and then dilute it with 2% maintenance solution to a concentration of 10 mg·mL, respectively. -1 , 2 mg·mL -1 , 0.4 mg·mL -1 , 0.08mg·mL -1 , 0.016mg·mL -1 , 0.0032mg·mL -1 , 0.0006mg·mL -1 , 0.0001mg·mL -1 . Five human cancer cells of colon cancer HCT-116, ovarian cancer HO-8910, gastric cancer SGC-7901, liver BEL-7402 and liver cancer SMMC-7721 in logarithmic growth phase were taken, and observed every other day after passage, and the cells were grown to the whole After 80% of the culture flask was trypsinized, 100 μL of cell suspension per well was seeded in a 96-well plate. at 37°C, 5% CO 2 , After culturing in RPMI1640 medium for 24h, rinse the 96-well plate with saline, 100 μL per well, shake evenly and aspirate. Then, the drug concentrations...

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Abstract

The invention discloses typhonium giganteum supercritical fluid extract with anticancer activity and a preparation method thereof. The extraction method is characterized in that the active ingredients of dry typhonium giganteum tuber powder serving as a raw material are extracted by using a supercritical fluid extraction technique and by regulating the parameters including flow of CO2, temperatures and pressures of an extraction kettle and a separation kettle and the like. The gas chromatography-mass spectrometer analysis on the extract indicates that the extract contains 40.22 percent of beta-sitosterol and 18.45 percent of campesterol. The results of microbiology and test technology (MTT) tests indicate that the typhonium giganteum extract obtained by the method has obvious tumor inhibition activity for cancer cell lines including colorectal cancer HCT-116, ovarian cancer HO-8910, gastric cancer SGC-7901, liver cancer BEL-7402 and liver cancer SMMC-7721. The extraction method has the advantages of high extraction selectivity, high extraction efficiency, high extraction speed, no pollution to extract, simplicity and convenience for operation, environment friendliness and the like. The extraction method disclosed by the invention can be used for extracting the anticancer active components in the typhonium giganteum and can be used an effective means for extracting sterols.

Description

Technical field [0001] The invention relates to a supercritical fluid extract of unicorn lotus tubers with anticancer activity and a preparation method thereof. The extraction method can be used for the extraction of anticancer active components in unicorn lotus tubers and the extraction of sterol substances. The main components are β-sitosterol and campesterol, especially the extract has high inhibitory activity on cancer cells, can be used in the development of potential anticancer drugs, and belongs to the field of medicine. Background technique [0002] The supercritical fluid extraction technology that emerged in the 1960s has been widely used in the fields of food, medicine, spices, petroleum, chemical industry, etc. , solvent extraction, etc.), supercritical fluid extraction has good separation selectivity, high extraction efficiency, good product purity and no organic solvent residue, complete biological activity of the product, simple process flow, low operating tem...

Claims

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Application Information

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IPC IPC(8): A61K36/888A61K31/575C07J9/00A61P35/00
CPCY02P20/54
Inventor 李庆勇祖元刚张宝友王春成宋瑱姜春菲
Owner NORTHEAST FORESTRY UNIVERSITY
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