A method for studying the interaction between microRNA and protein

A protein interaction and differential protein technology, applied in biochemical equipment and methods, special data processing applications, microbial measurement/inspection, etc., can solve problems such as abnormal protein expression and complex regulatory mechanisms of interacting human diseases

Inactive Publication Date: 2011-12-07
SHANGHAI CLUSTER BIOTECH
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Problems solved by technology

[0004] MicroRNA is more common in human cells. Many medical studies have shown that microRNA is likely to be involved in the formation of various human diseases. It can cause abnormal expression of proteins in human cells through direct regulation or indirect influence, thus leading to diseases.
However, in actual biological experimental research, due to the variety of types, forms and functions of biological substances, it is complicated to study the interaction between microRNA and protein and the regulation mechanism of human diseases.

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  • A method for studying the interaction between microRNA and protein
  • A method for studying the interaction between microRNA and protein
  • A method for studying the interaction between microRNA and protein

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Embodiment Construction

[0016] The present invention will take the research on the interaction between mir-144 and protein as an example to introduce the method of the present invention—a method for research on the interaction between microRNA and protein, and specific implementation steps.

[0017] Step 1. In this example, we selected Huh7 cells, a liver cancer cell line with high expression of mir-144, as experimental samples, applied mir-144 inhibitors and controls to the cells, transfected Huh7 cells, and performed DIGE (fluorescent differential electrophoresis) After staining, the differential proteins were screened, and the mass spectrometry data of the differential proteins in the sample were obtained, a total of 19 differentially expressed proteins.

[0018] Step 2, predicting the target gene of mir-144. Here, in order to expand the scope of the results, we select the four most commonly used analysis software:

[0019] 1. microT 3.0 (http: / / diana.cslab.ece.ntua.gr / microT / )

[0020] 2. miRan...

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Abstract

The present invention designs a method for studying the interaction between microRNA and protein, extracts the concept of an "indirect" target gene model, and conducts interaction research based on existing literature reports. The basic process is: Step 1, select microRNA For highly expressed cells, screen for differential proteins; step 2, prediction of microRNA target genes; step 3, introduce literature mining technology for differential protein and microRNA target genes, and build interaction network; step 4, integrate analysis results, and establish microRNA Regulatory pathways with proteins; step 5, introduce literature mining, search for literature reports that have been verified by experiments, and verify the above regulatory pathways.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for studying the interaction between microRNA and protein based on literature mining technology. Background technique [0002] MicroRNA (micro ribonucleic acid) is an endogenous non-coding microRNA with a length of 18-25 nucleotide single strands. It mainly inhibits its translation by completely or incompletely pairing with the 3' untranslated region of the target gene, thereby participating in the regulation of life activities such as individual development, cell apoptosis, proliferation and differentiation. Under pathological conditions, microRNAs can affect the occurrence and development of tumors by regulating their target genes and the signaling pathways they participate in, and play a role similar to oncogenes or tumor suppressor genes. Experiments have shown that in many tumors, some types of microRNAs are highly expressed, and these highly expressed microRNAs are rela...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/02G01N33/68G06F19/24
Inventor 曾华宗
Owner SHANGHAI CLUSTER BIOTECH
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