A method for studying the interaction between microRNA and protein
A protein interaction and differential protein technology, applied in biochemical equipment and methods, special data processing applications, microbial measurement/inspection, etc., can solve problems such as abnormal protein expression and complex regulatory mechanisms of interacting human diseases
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[0016] The present invention will take the research on the interaction between mir-144 and protein as an example to introduce the method of the present invention—a method for research on the interaction between microRNA and protein, and specific implementation steps.
[0017] Step 1. In this example, we selected Huh7 cells, a liver cancer cell line with high expression of mir-144, as experimental samples, applied mir-144 inhibitors and controls to the cells, transfected Huh7 cells, and performed DIGE (fluorescent differential electrophoresis) After staining, the differential proteins were screened, and the mass spectrometry data of the differential proteins in the sample were obtained, a total of 19 differentially expressed proteins.
[0018] Step 2, predicting the target gene of mir-144. Here, in order to expand the scope of the results, we select the four most commonly used analysis software:
[0019] 1. microT 3.0 (http: / / diana.cslab.ece.ntua.gr / microT / )
[0020] 2. miRan...
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