Optical information analyzing device and optical information analyzing method

Abstract

An optical information analyzer (10) includes a light emitting unit (30) that emits light (excitation light) (L0) to a sample (S), a transmission light receiving unit (50) that receives transmission light (L1), which is the excitation light passing through the sample (S), and detects the received transmission light as a transmission light signal (SG1), scattered light / fluorescence receiving units (60) and (70) that are provided at a plurality of positions, receive side scattered light / fluorescence components (L2) and (L3) from the sample (S), and detect the received side scattered light / fluorescence components as scattered light / fluorescence signals (SG2) and (SG3), and an analyzing unit (90) that measures the optical information of the sample (S) on the basis of the detected scattered light / fluorescence signals (SG2) and (SG3) and the detected transmission light signal (SG1) and analyzes the sample (S) on the basis of the measured optical information.

Description

technical field [0001] The present invention relates to an optical information analysis device and an optical information analysis method. In particular, it relates to an optical information analysis device and an optical information analysis method for measuring optical information of a sample by irradiating irradiation light onto a sample as a measurement target dispersed in a liquid flowing in a channel. Background technique [0002] A device has been proposed (for example, refer to Patent Document 1) in which a liquid containing dyed biological particles (microscopic objects to be examined: specimen) flows through a flow path of a flow path forming member (flow cell) The biological particle is irradiated with the light emitted by the illuminating part (irradiating part), and the scattered light or fluorescent light from the biological particle is detected by the detecting part (light receiving part), thereby obtaining the biological information of the biological particle...

AI Technical Summary

Problems solved by technology

[0005] However, in the conventional device as described above, when the sample flow passes through a position deviated from the center of the flow channel in the flow cell, since the optical axis of the irradiating part is aligned with the sample flow, the light-receiving part may be distorted. The problem that the optical axis of the optical system does not cross the optical axis of the sample flow and the irradiation part

For this reason, the light receiving unit is configured to receive light in a wide range. As a result, the sensitivity of detected scattered light or fluorescence decreases, or the obtained optical information of the specimen varies.

[0006] In addition, in the conventional apparatus as described above, since only one light receiving unit for receiving side scattered light and / or fluorescent light is provided, there may be problems caused by the orientation of the specimen when the specimen is not spherical. Sensitivity errors of scattered light or fluorescence, or sensitivity errors of scattered light or fluorescence caused by the displacement of the sample from the center position in the sample flow when the sample does not pass through the center position in the sample flow, etc. The problem of biased optical information of the specimen

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