A kind of microbial desilting agent and preparation method thereof
A desilting agent and microbial technology, applied in the field of microbial desilting agent and its preparation, can solve the problems of destroying the ecological environment, high cost, loss, etc., and achieve the effect of prolonging the effective time, low production cost and mild conditions
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Embodiment 1
[0028] Actinomycetes, Nitrosomonas europaea, Nitrobacter verdeii, Paracoccus pantrophus and Bacillus megaterium are selected as the microbial desilting agent in this embodiment as desilting bacteria, and porous sodium activated zeolite with a size of 40 meshes to 250 meshes is selected As a fixed carrier for dredging bacteria.
[0029] The present embodiment mainly carries out the screening of above-mentioned five kinds of dredging bacteria and the preparation of seed liquid:
[0030] Due to different microorganisms, different metabolic capacities, and different growth rates, if they are mixed and cultured, there will be growth competition, and they are easy to contaminate bacteria, so the culture of microorganisms must be single-plant culture. The preparation process of above-mentioned five kinds of dredging bacteria seed liquids is:
[0031] The five kinds of dredging bacteria were added to the five screening media respectively, and placed in a 35℃~37℃ incubator for...
Embodiment 2
[0033] This embodiment selects the dredging bacteria seed liquid prepared in Example 1 to prepare a microbial dredging agent, which comprises the following steps:
[0034] (1) Pretreatment of zeolite: use excess sodium salt solution (which can be NaCl, Na 2 SO 4 , NaNO 3 etc) processing so that Na + The exchange rate is above 75%, then drying at 90℃~120℃ for 2~3 hours, and then heating at 400℃~600℃ for 2~3 hours to obtain porous sodium activated zeolite particles; The adsorption capacity of the gas is improved, which is even larger than that of the synthetic 5A molecular sieve.
[0035] (2) Sterilization: Five kinds of liquid culture medium, pH regulators (ammonia, hydrochloric acid, etc.) for culturing the Actinomycetes, Nitrosomonas europaea, Nitrobacter verdeii, Paracoccus pantrophus and Bacillus megaterium respectively. ) and other utensils and reagents are subjected to high pressure (121℃, 0.1Mpa) steam sterilization, and then cooled to 25℃~37℃; ...
Embodiment 3
[0043] This example provides the use method and some application data of the microbial dredging agent prepared in Example 2, as shown in Table 1.
[0044] Table 1 The use method and application data of the microbial dredging agent prepared in Example 2
[0045]
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